Transplantation of neonatal cardiomyocytes plus fibrin sealant restores myocardial function in a rat model of myocardial infarction.

Abstract:

BACKGROUND:Most cardiac regenerative approaches can restore injured heart muscles. In this study, we investigated if fibrin sealant could help neonatal cardiomyocytes restore myocardial function in a rat model of myocardial infarction. METHODS:The left anterior descending artery in adult female Sprague-Dawley (SD) rats was ligated to make a myocardial infarction model. Neonatal ventricular cardiomyocytes from one-day male SD rats were isolated, labeled and cultured. The cells were injected into the infarcted area three weeks later. The animals were randomized into four recipient groups: (1) cardiomyocytes plus fibrin sealant (group CF, n = 10); (2) cardiomyocytes alone (group C, n = 10); (3) fibrin sealant recipients alone (group F, n = 10); (4) control group (n = 10). Four weeks after transplantation, echocardiography and Langerdoff model were used to assess heart function. Immunohistochemical staining and polymerase chain reaction (PCR) were performed to track the implanted cardiomyocytes and detect the sex-determining region Y gene on Y chromosome. RESULTS:Echocardiography showed the fraction shortening (FS) in groups CF, C, F and control group was (27.80 +/- 6.32)%, (22.29 +/- 4.54)%, (19.24 +/- 6.29)% and (20.36 +/- 3.29)% respectively with statistically significant differences in group CF compared with the other groups (P < 0.05). The Langendoff model revealed that the left ventricular development of peak pressure (LVDPmax, mmHg) in groups CF, C, F and control group was 104.81 +/- 17.05, 80.97 +/- 21.60, 72.07 +/- 26.17 and 71.42 +/- 17.55 respectively with statistically significant differences in group CF compared with the other groups (P < 0.05). Pathological examination and PCR indicated that transplanted cardiomyocytes in group CF survived better than those in the other groups. CONCLUSION:Transplanted neonatal cardiomyocytes plus fibrin sealant can survive in myocardial infarctioned area and improve heart function greatly in rat models.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Li YS,Gao BR

subject

Has Abstract

pub_date

2007-11-20 00:00:00

pages

2022-7

issue

22

eissn

0366-6999

issn

2542-5641

journal_volume

120

pub_type

杂志文章
  • C-arm-based flat-panel detector cone-beam computed tomography venography in the diagnosis of iliac vein compression syndrome.

    abstract:BACKGROUND:C-arm-based flat-panel detector cone-beam computed tomography (CBCT) venography has never been used in the management of iliac vein compression syndrome (IVCS). This study aimed to determine the technical feasibility and safety of CBCT venography in the diagnosis of IVCS compared with conventional venography...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000001046

    authors: Wang CM,Zhang WL,Li X

    更新日期:2020-08-26 00:00:00

  • Effects of prostaglandin E2 and all-trans retinoic acid combination on induced differentiation of human acute promyelocytic leukemia NB4 cells.

    abstract:OBJECTIVE:A human promyelocytic leukemia (APL) cell line NB4 was used to demonstrate the synergistic effects between all-trans retinoic acid (ATRA) and prostaglandin E2 (PGE2) on growth inhibition and cytodifferentiation induction. METHODS:NB4 cells were cultured in the presence of either ATRA or PGE2 as a single agen...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chen Z,Breitman TR

    更新日期:1997-10-01 00:00:00

  • Altered regional homogeneity in spontaneous cluster headache attacks: a resting-state functional magnetic resonance imaging study.

    abstract:BACKGROUND:Functional neuroimaging study has opened an avenue for exploring the pathophysiology of cluster headache (CH). The aim of our study was to assess the changes in brain activity in CH patients by the regional homogeneity method using resting-state functional magnetic resonance imaging technique. METHODS:The f...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Qiu EC,Yu SY,Liu RZ,Wang Y,Ma L,Tian LX

    更新日期:2012-02-01 00:00:00

  • Synchronous intraoperative radiofrequency ablation for multiple liver metastasis and resection of giant solid pseudopapillary tumors of the pancreas.

    abstract::The solid pseudopapillary tumors of the pancreas (SPTP) are rare tumors, which are commonly found in adolescent women. Radical surgical resection of the primary tumor or metastases is the standard treatment for SPTP and could achieve long-term survival. We reported a case of a 20-year-old female with multiple liver me...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li JX,Wu H,Huang JW,Prasoon P,Zeng Y

    更新日期:2012-05-01 00:00:00

  • Safety and efficacy of a novel technique in the use of fractional flow reserve in complex coronary artery lesions.

    abstract:BACKGROUND:Fractional flow reserve (FFR) has become an increasingly important index when making decisions with respect to revascularization of coronary artery stenosis. However, the pressure guidewire used in obtaining FFR measurements is difficult to control and manipulate in certain complex coronary artery lesions, r...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.152664

    authors: He WM,Li CL,Sun Y,Zhou Z,Mai YF

    更新日期:2015-03-20 00:00:00

  • Endolymphatic sac papillary tumor: a case report.

    abstract::Endolymphatic sac papillary tumor (ELST) is an extremely rare and aggressive tumor characterized by hearing loss and temporal bone destruction. A case with clinical, imaging, pathologic and treatment data is reported and relevant literature is reviewed. A 25-year-old woman, with ELST underwent craniotomy for tumor sub...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:

    authors: Yu SJ,Chen YD,Gao F,Qiu XG,Chang H

    更新日期:2011-11-01 00:00:00

  • Correlation between Electroencephalogram Alterations and Frontal Cognitive Impairment in Esophageal Cancer Patients Complicated with Depression.

    abstract:BACKGROUND:Some esophageal cancer patients complicated with depression exhibit cognitive impairments. Frontal electroencephalogram (EEG) may be used as a reliable biomarker for prefrontal-mediated cognitive functions. This study was to investigate alterations of EEG and frontal cognitive impairment in esophageal cancer...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.211552

    authors: Cao Y,Chen X,Xie H,Zou L,Hu LJ,Zhou XJ

    更新日期:2017-08-05 00:00:00

  • Differential Diagnosis of Two Chinese Families with Dyschromatoses by Targeted Gene Sequencing.

    abstract:BACKGROUND:The dyschromatoses are a group of disorders characterized by simultaneous hyperpigmented macules together with hypopigmented macules. Dyschromatosis universalis hereditaria (DUH) and dyschromatosis symmetrica hereditaria are two major types. While clinical and histological presentations are similar in these ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.172564

    authors: Liu JW,Asan.,Sun J,Vano-Galvan S,Liu FX,Wei XX,Ma DL

    更新日期:2016-01-05 00:00:00

  • Comparative study of optic disc measurement by Copernicus optical coherence tomography and Heidelberg retinal tomography.

    abstract:BACKGROUND:Copernicus optical coherence tomography (SOCT) is a new, ultra high-speed and high-resolution instrument available for clinical evaluation of optic nerve. The purpose of the study was to compare the agreements between SOCT and Heidelberg retinal tomography (HRT). METHODS:A total of 44 healthy normal volunte...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Yang QS,Yu YJ,Li SN,Liu J,Hao YJ

    更新日期:2012-08-01 00:00:00

  • Clinicopathological characteristics and prognostic factors of intrahepatic biliary cystadenocarcinoma.

    abstract:BACKGROUND:Surgical resection is generally considered the main curative treatment for intrahepatic biliary cystadenocarcinoma (IBCA) or suspected IBCAs, but controversy exists regarding the prognosis for IBCAs. This study aimed to describe the clinicopathological characteristics of IBCA and identify prognostic factors ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.156108

    authors: Xu MY,Shi XJ,Wan T,Liang YR,Wang HG,Zhang WZ,He L,Chen MY,Lyu SC,Zhang WW,Li HX

    更新日期:2015-05-05 00:00:00

  • Sex hormone receptors of hemangiomas in children.

    abstract:OBJECTIVE:To investigate whether or not estrogen receptor and other sex hormone receptors are present in hemangioma tissues of children. METHODS:Fifty-two specimens of hemangiomas were taken from 52 children. The specimens were doubly stained with hematoxylin-eosin and enzyme-linking affinity immunohistochemistry. The...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Lui W,Zhang S,Hu T,Wei F,Gao Y,Cheng N

    更新日期:1997-05-01 00:00:00

  • A new modification of transanal Soave pull-through procedure for Hirschsprung's disease.

    abstract:BACKGROUND:One stage transanal Soave pull-through procedure (TSPP) is a recent popular operation in the treatment of Hirschsprung's disease (HD). With no visible scar and a short hospital stay, it is well accepted by surgeons and mothers. In the conventional Soave procedure, a long rectal muscular cuff left for anocoli...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li AW,Zhang WT,Li FH,Cui XH,Duan XS

    更新日期:2006-01-05 00:00:00

  • Prognostic factors and therapeutic efficacy of combined radio-chemotherapy in Waldeyer's ring non-Hodgkin lymphoma.

    abstract:OBJECTIVE:To improve the prognosis and therapeutic efficacy of Waldeyer's ring non-Hodgkin lymphoma (NHL-WR), combined radio-chemotherapy was used to treat the patients with NHL-WR and prognostic factors were analyzed. METHODS:Ninety patients with stage I-IV NHL-WR were treated with combined radio-chemotherapy. 4000 c...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Yong W,Zhang Y,Zheng W,Wei Y

    更新日期:2000-02-01 00:00:00

  • In vitro chondrogenesis of the goat bone marrow mesenchymal stem cells directed by chondrocytes in monolayer and 3-dimetional indirect co-culture system.

    abstract:BACKGROUND:Cartilage injury has a very poor capacity for intrinsic regeneration. The cell-based treatment strategy for the cartilage repair using differentiated bone marrow mesenchymal stem cells (BMSCs) is, however, a promising approach to the chondral repair. This study was aimed to explore the chondrogenic potential...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li JW,Guo XL,He CL,Tuo YH,Wang Z,Wen J,Jin D

    更新日期:2011-10-01 00:00:00

  • An analysis of prognostic factors in cirrhosis.

    abstract::To develop a more comprehensive index for predicting the prognous of liver cirrhosis. 300 consecutive patients with cirrhosis were studied in terms of survival from 1975 to 1986. Median follow-up period was 5.3 years. A multivariable survival analysis (Cox's regression model) using clinical biochemical data obtained a...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang JY,Liu HY,Zhu WN,Han JQ,Dong CY,Shi YJ,Cai HJ,Cao JM,Yi QL

    更新日期:1989-04-01 00:00:00

  • Disseminated penicilliosis marneffei in China. Report of three cases.

    abstract::Three cases with disseminated Penicilliosis marneffei (PSM) are reported. They are all natives of Guangxi Zhuang Autonomous Region. Among them, 2 died and 1 remains alive. Their clinical features were characterized by multiple organ involvement, multiple subcutaneous abscesses, inflammatory papules, nodules and pustul...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li JS,Pan LQ,Wu SX,Su SX,Su SB,Shan LY

    更新日期:1991-03-01 00:00:00

  • Activation and coreceptor expression of T lymphocytes induced by highly active antiretroviral therapy in Chinese HIV/AIDS patients.

    abstract:BACKGROUND:At the end of 2005, 650,000 people lived with human immunodeficiency virus type-1 (HIV-1) in China, of whom 75 000 were AIDS patients. Many AIDS patients received highly active antiretroviral therapy (HAART) supported by the "China CARES" program but the immune responses of HAART were seldom reported. This s...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zhang ZN,Shang H,Jiang YJ,Liu J,Dai D,Diao YY,Geng WQ,Jin X,Wang YN

    更新日期:2006-12-05 00:00:00

  • Prostate specific membrane antigen knockdown impairs the tumorigenicity of LNCaP prostate cancer cells by inhibiting the phosphatidylinositol 3-kinase/Akt signaling pathway.

    abstract:BACKGROUND:Prostate specific membrane antigen (PSMA) can facilitate the growth, migration, and invasion of the LNCaP prostate cancer cell lines, but the underlying molecular mechanisms have not yet been clearly defined. Here, we investigated whether PSMA serves as a novel regulator of the phosphatidylinositol 3-kinase ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Guo Z,Lai Y,Du T,Zhang Y,Chen J,Bi L,Lin T,Liu H,Wang W,Xu K,Jiang C,Han J,Zhang C,Dong W,Huang J,Huang H

    更新日期:2014-01-01 00:00:00

  • Dynamic infusion cavernosometry and cavernosography for classifying venous erectile dysfunction and its significance for individual treatment.

    abstract:BACKGROUND:The precise pathophysiology of venous erectile dysfunction (VED) was still unclear. Dynamic infusion cavernosometry and cavernosography (DICC) was the gold standard approach for the diagnoses of VED. However, a standard operative procedure of DICC was needed and it was unclear whether DICC could show promise...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000000099

    authors: Gao QQ,Chen JH,Chen Y,Song T,Dai YT

    更新日期:2019-02-01 00:00:00

  • Secreted modular calcium-binding proteins in pathophysiological processes and embryonic development.

    abstract:OBJECTIVE:Secreted modular calcium-binding proteins (SMOCs) are extracellular glycoproteins of the secreted protein, acidic, and rich in cysteine-related modular calcium-binding protein family and include two isoforms, SMOC1 and SMOC2, in humans. Functionally, SMOCs bind to calcium for various cell functions. In this r...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:10.1097/CM9.0000000000000472

    authors: Gao Q,Mok HP,Zhuang J

    更新日期:2019-10-20 00:00:00

  • Aminoglycoside ototoxicity in three murine strains and effects on NKCC1 of stria vascularis.

    abstract:BACKGROUND:After establishing a murine model of aminoglycoside antibiotic (AmAn) induced ototoxicity, the sensitivity of AmAn induced ototoxicity in three murine strains and the effect of kanamycin on the expression of Na-K-2Cl cotransporter-1 (NKCC1) in stria vascularis were investigated. METHODS:C57BL/6J, CBA/CaJ, N...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chu HQ,Xiong H,Zhou XQ,Han F,Wu ZG,Zhang P,Huang XW,Cui YH

    更新日期:2006-06-20 00:00:00

  • Clinical study of inflammatory factors in sputum induced early after lung volume reduction surgery.

    abstract:BACKGROUND:The aim of this study was to prospectively study the changes in neutrophil elastase (NE), fibroblast growth factor 9 (Fgf9), matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase 1 (TIMP-1) in sputum induced during the early period after lung volume reduction surgery (LVRS). METHODS:From...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Miao JB,Hou SC,Li H,Hu B,Wang TY

    更新日期:2008-09-20 00:00:00

  • Genital Ureaplasma urealyticum infection in varicocele-related infertility.

    abstract:OBJECTIVE:To assess the existence of a possible infectious factor in varicocele-related infertility. METHODS:A total of 925 seminal plasmas from 590 infertile men with varicocele and 335 infertile patients without palpable varicocele were cultured for Ureaplasma urealyticum infection, and routine semen analysis was ma...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li H,Guo Y,Wang Y,Sun X

    更新日期:1997-11-01 00:00:00

  • Recombinant human bone morphogenetic protein-2 promotes the proliferation of mesenchymal stem cells in vivo and in vitro.

    abstract:BACKGROUND:Bone morphogenetic protein (BMP) is a member of the superfamily of transforming growth factor-beta. Recent studies show that it is an indispensable factor in hematopoiesis. To better characterize the effect of recombinant human BMP (rhBMP)-2 in hematopoiesis, we set out to determine whether rhBMP-2 could pro...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Liu SB,Hu PZ,Hou Y,Li P,Cao W,Tian Q

    更新日期:2009-04-05 00:00:00

  • Over-expression of c-fos mRNA in the hippocampal neurons in Alzheimer's disease.

    abstract:OBJECTIVE:To analyze the relationship between the proto-oncogene c-fos change and neuronal degeneration in the hippocampus of the patients with Alzheimer's disease. METHODS:The post mortem human hippocampal tissues were divided into three groups, namely, the aged, the young and Alzheimer's disease (AD) groups. Each gr...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Lu W,Mi R,Tang H,Liu S,Fan M,Wang L

    更新日期:1998-01-01 00:00:00

  • Therapeutic antitumor response to cervical cancer in mice immunized with U14 vaccines transfected with costimulatory B7 gene.

    abstract:OBJECTIVE:To investigate the effect of U14 vaccine transfected with the B7 gene in inducing antitumor immune response to murine cervical carcinoma in Chinese 615-strain mice. METHODS:A recombinant retroviral plasmid vector expressing mouse B7-1 gene (pLNSX-mB7) was transfected into 615-strain mouse cervical carcinoma ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Tao G,Hu J,Zou H,Lin Q,Liu F,Wu Y,Sun Q

    更新日期:2001-06-01 00:00:00

  • Methicillin-resistant Staphylococcus aureus pneumonia in diabetics: a single-center, retrospective analysis.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) pneumonia is an important issue with significant morbidity and mortality in clinical practice, especially in diabetes mellitus (DM). Studies focusing on S. aureus pneumonia in DM is limited, we sought to make a relatively comprehensive exploration of clinica...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000000270

    authors: Zhang QR,Chen H,Liu B,Zhou M

    更新日期:2019-06-20 00:00:00

  • Neuroprotective effect of exogenous vascular endothelial growth factor on rat spinal cord neurons in vitro hypoxia.

    abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is well known as a hypoxia-induced protein. That it markedly increased expression of VEGF and improvement of rat motor function after spinal cord injury suggested that VEGF could play a neuroprotective role in ischaemic tolerance. This study investigated whether vasc...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Ding XM,Mao BY,Jiang S,Li SF,Deng YL

    更新日期:2005-10-05 00:00:00

  • [Changes after dynamic observation of ultrastructural cerebral infarction in renovascular hypertensive rats].

    abstract:OBJECTIVE:To study the ultrastructural damages of brain tissues dynamically after middle cerebral artery obstruction (MCAO). METHODS:A middle cerebral artery obstruction (MCAO) model was reproduced in renal vascular hypertensive rats (RHR). Brain tissues in different locations were collected for eight times in row, fr...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Fang Y,Huang R,Su Z,Lin J,Li J

    更新日期:2000-06-01 00:00:00

  • Oxygen administration in the care of neonates: a double-edged sword.

    abstract:OBJECTIVE:To evaluate the use of oxygen in neonates in the past, present and future. DATA SOURCES:The data are mainly from Pubmed with relevant published articles from the 1940s to the present with some information gathered from web searches. STUDY SELECTION:Studies evaluating the use of oxygen in premature and term ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:

    authors: Dennery PA

    更新日期:2010-10-01 00:00:00