Abstract:
BACKGROUND:Prostate specific membrane antigen (PSMA) can facilitate the growth, migration, and invasion of the LNCaP prostate cancer cell lines, but the underlying molecular mechanisms have not yet been clearly defined. Here, we investigated whether PSMA serves as a novel regulator of the phosphatidylinositol 3-kinase (PI3K)/Akt signaling by employing PSMA knockdown model and PI3K pharmacological inhibitor (LY294002) in LNCaP prostate cancer cells. METHODS:PSMA knockdown had been stably established by transfecting with lentivirus-mediated siRNA in our previous study. Then, LNCaP cells were divided into interference, non-interference, and blank groups. We first testified the efficacy of PSMA knockdown in our LNCaP cell line. Then, we compared the expression of PSMA and total/activated Akt by Western blotting in the above three groups with or without LY294002 treatment. Furthermore, immunocytochemistry was performed to confirm the changes of activated Akt (p-Akt, Ser473) in groups. Besides, cell proliferation, migration, and cell cycle were measured by CCK-8 assay, Transwell analysis, and Flow cytometry respectively. RESULTS:After PSMA knockdown, the level of p-Akt (Ser473) but not of total-Akt (Akt1/2) was significantly decreased when compared with the non-interference and blank groups. However, LY294002 administration significantly reduced the expression of p-Akt (Ser473) in all the three groups. The results of immunocytochemistry further confirmed that PSMA knockdown or LY294002 treatment was associated with p-Akt (Ser473) down-regulation. Decrease of cell proliferation, migration, and survival were also observed upon PSMA knockdown and LY294002 treatment. CONCLUSIONS:Taken together, our results reveal that PI3K/Akt signaling pathway inhibition may serve as a novel molecular mechanism in LNCaP prostate cancer cells of PSMA knockdown and suggest that Akt (Ser473) may play a critical role as a downstream signaling target effector of PSMA in this cellular model.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Guo Z,Lai Y,Du T,Zhang Y,Chen J,Bi L,Lin T,Liu H,Wang W,Xu K,Jiang C,Han J,Zhang C,Dong W,Huang J,Huang Hsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
929-36issue
5eissn
0366-6999issn
2542-5641journal_volume
127pub_type
杂志文章abstract:BACKGROUND:Atypical meningioma is one of the rare subtypes of meningioma, which is lacking of optimal consensus on treatment strategies. This study aimed to investigate the radical treatment strategies to improve the long-term outcome of recurrent atypical meningiomas. METHODS:The prognostic factors including the age ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:Recent studies have demonstrated that the Lenke system is relatively efficient and consistent in classifying scoliosis curves. Basically, fusion should include the main curve and the structural minor curve. The criteria for defining the structural minor curve were established to help guide these decision-mak...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract:Background:Recent advances in extracorporeal membrane oxygenation (ECMO) have led to increasing interest in its use during cardiopulmonary resuscitation (CPR). However, decisions regarding extracorporeal CPR (ECPR) in children are difficult as a result of limited studies, especially in Asia Pacific. The objective of th...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.233946
更新日期:2018-06-20 00:00:00
abstract:BACKGROUND:Pulsed radiofrequency (PRF), as a non-invasive treatment of neuropathic pain (NP), has been widely administered clinically. Previous studies have shown that PRF has the potential to improve hyperalgesia in animal models of NP. However, there have been few reports to clarify whether the mechanism of PRF treat...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000619
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abstract:ABSTRACT:Cerebral small vessel disease (SVD) is a common global brain disease that causes cognitive impairment, ischemic or hemorrhagic stroke, problems with mobility, and neuropsychiatric symptoms. The brain damage, seen as focal white and deep grey matter lesions on brain magnetic resonance imaging (MRI) or computed ...
journal_title:Chinese medical journal
pub_type: 杂志文章
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更新日期:2020-10-06 00:00:00
abstract:OBJECTIVE:To investigate molecular events of exocytosis in cultured human pheochromocytoma cells with stimulation. METHODS:The cultured pheochromocytoma cells prepared from human adrenal pheochromocytoma tumor were stimulated for the release of catecholamines by depolarization with the administration of 50 mmol/L KCl....
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract:BACKGROUND:A number of studies have demonstrated the rates of overall and aneurysm-related mortality and morbidity in Western populations. The cardiovascular risk factors influencing postoperative outcome have been also reported. Until recently, little has been known about the prognosis in this patient cohort in the Ch...
journal_title:Chinese medical journal
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doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Some individuals have multiple renal arteries. Severe stenosis in one of the arteries may cause refractory hypertension. The detection of stenosis within one of the multiple renal arteries usually required invasive procedures, such as computed tomographic angiography (CTA) and magnetic resonance angiography ...
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doi:
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Survivin is an oncoprotein silenced in normal mature tissues but reactivated in serous ovarian cancer (SOC). Although transcriptional activation is assumed for its overexpression, the long 3'-untranslated region (3'-UTR) in survivin gene, which contains many alternate polyadenylation (APA) sites, implies a p...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.181965
更新日期:2016-05-20 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) core antigen assays have been produced to exclude infectious donations collected during the preseroconversion window phase (PWP). For the same purpose, we evaluated the specificity and sensitivity of a novel hepatitis C virus NS3 antigen detection immunoassay and the application of th...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-02-20 00:00:00
abstract:BACKGROUND:Early neurological deterioration (END) is a prominent issue after recanalization treatment. However, few studies have reported the characteristics of END after endovascular treatment (EVT) as so far. This study investigated the incidence, composition, and outcomes of END after intravenous recombinant tissue ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.222343
更新日期:2018-01-20 00:00:00
abstract:BACKGROUND:A free running asthma screening test is usually used for screening exercise-induced bronchoconstriction. The total serum allergen IgE level can reveal the patient's atopy characteristics. Our study is to evaluate the diagnostic values of the combination of the two tests in asthmatic children and compare this...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Thoracic endovascular aortic repair (TEVAR) is an emerging treatment modality, which has been rapidly embraced by clinicians treating thoracic aortic disease. However, the clinical manifestations of systemic inflammatory response after TEVAR as post-implantation syndrome (PIS) resemble the perioperative infe...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Diffusion tensor imaging (DTI) permits quantitative examination within the pyramidal tract (PT) by measuring fractional anisotropy (FA). To the best of our knowledge, the inter-variability measures of FA along the PT remain unexplained. A clear understanding of these reference values would help radiologists ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:In county-level tuberculosis (TB) dispensaries in China, the accurate diagnosis of sputum smear-negative pulmonary tuberculosis (SNPT) needs to be improved by developing and validating clinical and radiographic predictors. METHODS:The study was conducted simultaneously in three counties per province in Chon...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Tumor necrosis factor-α is a key mediator in the pathogenesis of psoriasis. Infliximab is a monoclonal antibody that specifically binds to tumor necrosis factor-α. The purpose of this study was to validate the efficacy and safety of 5 mg/kg infliximab therapy in Chinese patients with moderate to severe plaqu...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究,随机对照试验
doi:
更新日期:2012-06-01 00:00:00
abstract::Ninety-five patients with perforation, hemorrhage or stenosis due to duodenal ulcer were treated by extended parietal cell vagotomy. Postoperative follow-up ranged from 3.5 to 10 years (mean 6 years) in 88 patients (92%) with acute perforation (60), hemorrhage (8) and stenosis (20). There was no operative mortality. U...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND:Double plate osteosynthesis is the standard treatment for intra-articular fractures of the distal humerus. However, there is still controversy concerning dual plate positions in terms of providing optimal stability. The purpose of this retrospective study was to compare the clinical outcomes in patients with...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-06-01 00:00:00
abstract::Twenty-seven samples of pleomorphic salivary adenoma (PSA) were examined for expression and structure of c-myc, c-Ha-ras, c-erbB-2, c-sis oncogenes by RNA dot blot, DNA dot blot and Southern blot hybridization. Eighteen of 25 PSA showed 5.02 +/- 4.74 fold overexpression of c-myc oncogene, 6 of 19 PSA had 4.91 +/- 3.87...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Immunohistochemical and ultrastructural findings in a primary intracranial endodermal sinus tumor are reported in this paper. The tumor cells exhibited AFP, CEA and anti-alpha-1-trypsin positive immunoreactivity immunocytochemically. Aggregates of electron-dense material in the extra- and intracellular spaces and amor...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract:OBJECTIVE:To characterize effects of the GP Ib alpha mutation (A156V) on its interaction with von Willebrand factor (vWf) under high fluid shear stress. METHODS:The residue A156 of GP Ib alpha was converted to a valine and the mutant expressed in CHO cells expressing wild-type GP Ib beta and GPIX. The transfected cell...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract:BACKGROUND:Benign prostate hyperplasia is one of the most common diseases affecting the health of the aging males. Watchful waiting is an acceptable management strategy for benign prostate hyperplasia in which the patient is monitored by the physician but receives no active intervention. The epidemiological data on thi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-12-20 00:00:00
abstract:OBJECTIVE:To study the prevalence of skin positivity to purified protein derivative (PPD) in human immunodeficiency virus (HIV)-infected patients in Hong Kong. METHODS:Consecutive clients of an HIV clinic were administered the PPD test and 2 units of PPD-RT23 were used. The area of induration was then measured in 48 t...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:BACKGROUND:Among patients with advanced multivessel coronary disease, left ventricular (LV) function is widely variable, and clinical and angiographic correlates of ventricular dysfunction remain to be defined. METHODS:Among 73 339 patients undergoing diagnostic cardiac catheterization at a single center in China, pat...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Ophthalmic gel has been developed to increase the drug concentration in aqueous humor and to retard the loss of drug from the conjunctival sac. The research was to compare the drug concentration in aqueous humor of cataract patients administered 0.3% gatifloxacin ophthalmic gel with that in patients administ...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2010-08-05 00:00:00
abstract:BACKGROUND:Interferon-gamma release assay (IGRA) has been used in latent tuberculosis (TB) infection and TB diagnosis, but the results from different high TB-endemic countries are different. The aim of this study was to investigate the value of IGRA in the diagnosis of active pulmonary TB (PTB) in China. METHODS:We co...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:10.4103/0366-6999.223860
更新日期:2018-02-05 00:00:00
abstract:BACKGROUND:The SYNergy between percutaneous coronary intervention with TAXus and cardiac surgery Score II (SS-II) can well predict 4-year mortality in patients with complex coronary artery disease (CAD), and guide decision-making between coronary artery bypass graft surgery and percutaneous coronary intervention (PCI)....
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.162510
更新日期:2015-08-20 00:00:00
abstract:BACKGROUND:Collagen VI-related myopathies are autosomal dominant and recessive hereditary myopathies, mainly including Ullrich congenital muscular dystrophy (UCMD) and Bethlem myopathy (BM). Muscle magnetic resonance imaging (MRI) has been widely used to diagnosis muscular disorders. The purpose of this study was to ev...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.186638
更新日期:2016-08-05 00:00:00
abstract:BACKGROUND:Both athletes with intensive exercise and aged people may have weakened immunity against virus infection. This study aimed to evaluate whether people undergoing aerobic exercises including competitive cyclists with moderate training (CMT) and middle-aged people practicing Tai Chi Chuan (TCC) exercise have hi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-11-05 00:00:00
abstract:BACKGROUND:Transradial coronary intervention has been widely used because of its effects in lowering the incidence of complications in vascular access site and improving patient satisfaction compared to the femoral approach. This study aimed to investigate the safety and feasibility of transradial approach for primary ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-05-05 00:00:00