Abstract：
BACKGROUND:Bone morphogenetic protein (BMP) is a member of the superfamily of transforming growth factor-beta. Recent studies show that it is an indispensable factor in hematopoiesis. To better characterize the effect of recombinant human BMP (rhBMP)-2 in hematopoiesis, we set out to determine whether rhBMP-2 could promote the proliferation of mesenchymal stem cells (MSCs) and increase the levels of hematopoietic cytokines in MSCs. METHODS:2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino) carbonyl)-2H-tetrazolium hydroxide (XTT), real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the effects of rhBMP-2 on the proliferation and hematopoietic cytokine levels of MSCs. In addition, MSCs marked with Hoechst33342 were transplanted into BALB/c mice by the intravenous route or intra-bone marrow transplantation, and cluster numbers were counted. RESULTS:The XTT test revealed that rhBMP-2 significantly induced proliferation of MSCs in doses ranging from 10 ng/ml to 0.1 mg/ml in a dose-dependent manner. The experiments in vivo showed that there were more clusters of donor cells in bone marrow, spleen, liver and lung of the BMP group than those in the control group after both intra-bone marrow transplantation (P < 0.001, P < 0.001, P < 0.001, and P = 0.001, respectively) and intravenous transplantation (P < 0.001, P < 0.001, and P < 0.001 respectively). The results of real-time PCR and ELISA revealed that rhBMP-2 significantly increased mRNA expressions and protein levels of IL-6, IL-7, IL-11, G-CSF, M-CSF and SCF. CONCLUSIONS:The treatment with rhBMP-2 promotes the proliferation of MSCs in vivo and in vitro and increases the levels of hematopoietic cytokines in MSCs, which may contribute to the improvement of hematopoietic function.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Liu SB,Hu PZ,Hou Y,Li P,Cao W,Tian Qsubject
Has Abstractpub_date
2009-04-05 00:00:00pages
839-43issue
7eissn
0366-6999issn
2542-5641journal_volume
122pub_type
杂志文章abstract：Background:Recent advances in extracorporeal membrane oxygenation (ECMO) have led to increasing interest in its use during cardiopulmonary resuscitation (CPR). However, decisions regarding extracorporeal CPR (ECPR) in children are difficult as a result of limited studies, especially in Asia Pacific. The objective of th...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.233946
更新日期：2018-06-20 00:00:00
abstract：BACKGROUND:Chronic graft-versus-host disease (GVHD), the commonest long-term complication after allogeneic hematopoietic stem cell transplantation (HSCT), has a negative impact on patients' health related quality of life (HRQoL). This study was designed to investigate the HRQoL in patients with chronic GVHD in China. ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：BACKGROUND:In 2008, infants in some areas of China suffered from stones of the urinary system which were caused by melamine-contaminated milk formula. Most of the infants were asymptomatic, and a few suffered from acute renal failure induced by urinary obstruction by stones. This study aimed to assess the significance ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-02-05 00:00:00
abstract：:134 fractures of the distal radius in 132 patients are reviewed to determine the incidence and influencing factors of coexisting carpal in stability. By measurement and analysis of the changes in carpal angles and joint spaces, carpal instability was discovered in 41 fractures, an incidence of 30.6%. Six patterns of i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：BACKGROUND:Respiratory failure caused by metastatic pulmonary choriocarcinoma usually develops rapidly and is associated with a high mortality. The clinical management strategy is important in choriocarcinoma patients with acute respiratory failure. The objective of this study was to evaluate the clinical characteristi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：BACKGROUND:Congenital uterine anomalies are associated with the highest incidence of reproductive failure and obstetric complications. This study aimed to summarize the clinical characteristics and prenatal outcome of pregnancy in women with congenital uterine malformations. METHODS:This retrospective study evaluated ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-02-20 00:00:00
abstract：:The environment structure of natural nidi and epidemic areas of hemorrhagic fever with renal syndrome (HFRS) possesses characteristics of topography, hydrology, climate, soil, vegetation, and animals. The natural nidi and epidemic areas of HFRS are distributed mainly in plain and hilly regions under 500 meters above t...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：BACKGROUND:Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma (NPC), but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the e...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.196586
更新日期：2017-01-05 00:00:00
abstract：BACKGROUND:Smoking is the major cause of airway inflammation in chronic obstructive pulmonary disease (COPD), and smoking cessation is regarded as one of the important strategies for prevention and treatment of the inflammation. The inflammation of the chronic airway may be present and deteriorated even if the COPD pat...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-09-05 00:00:00
abstract：BACKGROUND:Vascular endothelial dysfunction is considered a key pathophysiologic process for the development of acute lung injury. In this study, we aimed at investigating the effects of unfractionated heparin (UFH) on the lipopolysaccharide (LPS)-induced changes of vascular endothelial-cadherin (VE-cadherin) and the p...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000905
更新日期：2020-08-05 00:00:00
abstract：OBJECTIVE:To test the immunosuppressive effect of cyclosporine (Cs) in a polymer placed in the anterior chamber of corneal allograft recipients. METHODS:Wistar inbred rats with vascularized corneas were recipients of corneal allografts from Sprague-Dawley donor rats. Rats underwent penetrating keratoplasty and were di...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Injections of serotonin (5-HT) into the aortic arch of rats caused greater reductions of blood pressure (BP) and less increases in renal vascular resistance (RVR) than that into jugular veins, in contrast to norepinephrine (NE) which tended to cause larger increases in BP and RVR by the intra-aortic route. Indomethaci...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：BACKGROUND:The patients with unprotected left main coronary artery (ULMCA) stenosis and chronic total occlusion (CTO) lesions at the left anterior descending (LAD) artery are often recommended for bypass surgery. However, some of these patients are deemed inoperable or are at high risk for surgery. In this study, we ex...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-04-05 00:00:00
abstract：BACKGROUND:The usual transbronchial coagulation techniques include microwave, argon plasma coagulation (APC), electrocautery and cryotherapy. However, there are serious clinical problems in the safety of each. By analyzing the experimental data and clinical observations, we observed the variable effects of different co...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：: ...
journal_title：Chinese medical journal
pub_type： 信件
doi：10.4103/0366-6999.245261
更新日期：2018-11-20 00:00:00
abstract：BACKGROUND:The most important objective of transplant studies in the injured spinal cord has been to provide a favorable environment for axonal growth. Moreover, the continuing discovery of new grafts is providing new potentially interesting transplant candidates. Our purpose was to observe the morphological and functi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：OBJECTIVE:A human promyelocytic leukemia (APL) cell line NB4 was used to demonstrate the synergistic effects between all-trans retinoic acid (ATRA) and prostaglandin E2 (PGE2) on growth inhibition and cytodifferentiation induction. METHODS:NB4 cells were cultured in the presence of either ATRA or PGE2 as a single agen...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：OBJECTIVE:To summarize the progress of surgical treatment of hepatocellular carcinoma (HCC) and related basic research at the Liver Cancer Institute of Shanghai Medical University in the recent years, with special reference to recurrence and metastasis. METHODS:Published and unpublished update clinical and experimenta...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：1999-10-01 00:00:00
abstract：:The effect of exercise on pulmonary gas exchange, arterial oxygenation, oxygen transport and delivery, and hemodynamics are studied in 100 patients with chronic obstructive pulmonary diseases. According to the change of PaO2 or PaCO2 induced by exercise, patients were divided into two groups. Group A (n = 52) had sign...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Peroxisome proliferator-activated receptor (PPAR) alpha is one of the subtypes of PPARs. It regulates metabolism of lipid and lipoprotein, as well as glucose homeostasis. In addition, PPARalpha influences cellular proliferation, inflammation, differentiation and apoptosis, which plays a vital role in cardiov...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-08-20 00:00:00
abstract：BACKGROUND:Cesarean scar pregnancy (CSP) is a very rare but life-threatening entity and there is no optimal management strategy. Here we report a successfully conservative treatment of CSP. METHODS:We retrospectively analyzed the clinical data of 54 women with CSP, who underwent uterine artery embolization between Jan...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:The usefulness of in-phase/opposed-phase imaging and diffusion weighted imaging (DWI) in differentiating benign and neoplastic vertebral fractures has been described. In this study, we aimed to evaluate the influence of the severity of vertebral damage on the diagnostic performance of these two technologies....
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-06-05 00:00:00
abstract：BACKGROUND:Type 1 diabetes has been recognized as an organ specific autoimmune disease owing to the immune destruction of pancreatic islet beta cells in genetically susceptible individuals. In both human and rodent models of type 1 diabetes, such as nonobese diabetic (NOD) mice, biobreeding rats, the disease has a dist...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-08-05 00:00:00
abstract：:Using ambulatory electrocardiography (AE), the spontaneous variability in 40 cases of chronic stable premature ventricular complexes (PVCs) was studied. The mean hourly PVC frequencies over 24 hours ranged 2-1 682. When analysing pooled data, there was significant variance between different monitoring periods and betw...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：BACKGROUND:Marked interindividual variation exists in blood pressure response to benazepril, which is considered to have genetic basis. Our objectives were to evaluate whether the E112D polymorphism in the prolylcarboxypeptidase (PRCP) gene has impact on blood pressure response to benazepril. METHODS:Hypertensive pati...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-10-20 00:00:00
abstract：OBJECTIVE:To investigate the relationship between angiotensin-converting enzyme (ACE) gene insertion/deletion polymorphism and the clinico-pathological manifestations in patients with immunoglobulin nephropathy (IgAN). METHODS:A flanking primer pair and an insertion-specific primer pair were used to perform two polyme...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:100 women ranging in age from 26-34 years were followed up for 4-21 months after undergoing sterilization by tubal occlusion using intraluminal threads and silver clips. The number of children/woman ranged from 1-5 with 80% having 2. 31 women underwent sterilization postmenstrually, 11 midmenstrually, 28 premenstrua...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：BACKGROUND:Fluid dynamic mechanisms attributed to coronary bifurcation lesions remain a subject of study. The present study aimed at investigating the hemodynamic change of wall shear stress (WSS) in patients with coronary bifurcation lesions treated by double kissing (DK) crush or one-stent with final kissing balloon ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND:Bioactive proteins, such as cytokines and chemokines, have not been systematically evaluated in healthy and preeclamptic pregnancies. We aimed to investigate the difference of these proteins between healthy and preeclamptic pregnancies in order to help clarify their potential roles in the pathogenesis of hyp...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:P53 is one of the most studied tumor suppressors in the cancer research, and over 50% of human tumors carry P53 mutations. MDM-2 is amplified and/or overexpressed in a variety of human tumors of diverse tissue origin. The aim of this study was to examine the expression of P53 protein and MDM-2 protein in gli...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
