Abstract:
BACKGROUND:Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR), chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diagnostic (IVD) directive requires traceability to reference methods and materials. The purpose of this study was to verify the effect of traceability implementation by investigating the trueness of creatinine measurement on nine homogenous systems in Beijing. METHODS:Commutable frozen human serum reference material, National Institute of Standards & Technology (NIST) Standard Reference Material (SRM) 967, was used to verify the trueness of Scr measurement results from nine homogeneous analytical systems of seven companies which are the most widely used systems in Beijing's third-grade hospitals. The methods referred to the Jaffe's and Enzymatic methods. RESULTS:from nine routine measurement systems were assessed using two criteria: biological variability and Clinical Laboratory Improvement Amendments' 88 (CLIA' 88). We simulated a series of broken lines representing the limits of SD and bias that would produce a relative increase (or decrease) of 10% and 20% in the measurement error when estimating GFR (MEeGFR) using the isotope dilution mass spectrometry (IDMS)-traceable Modification of Diet in Renal Disease (MDRD) Study equation. RESULTS:of the College of American Pathologists (CAP) 2008-B LN24 Survey were compared with our investigation results. RESULTS:Compared with the total error criteria of biological variability, Ortho (traceable to IDMS) met the minimum acceptable criteria; Roche (Jaffe), Roche (Enzymatic), Shino and Daiichi met the desirable criteria at level I. At level II, Ortho (traceable to gas chromatography/isotope dilution mass spectrometry, GC/IDMS), Dade Behring and Beckman (traceable to rate Jaffe) met the minimum acceptable criteria; Roche (Enzymatic) met the optimum criteria. The other five systems met the desirable criteria. Compared with the second criterion, all the results met the requirement of CLIA' 88. Trueness evaluation showed: the MEeGFR of Dade Behring exceeded 10% while the MEeGFRs of Beckman (traceable to rate Jaffe), Beckman (traceable to IDMS) and Ortho (traceable to Jaffe/High Performance Liquid Chromatography) exceeded 20% at level I. At level II the MEeGFRs of Dade Behring, Ortho (traceable to GC/IDMS) and Beckman (traceable to rate Jaffe) exceeded 10%. None of the nine systems got a MEeGFR higher than 20%. The conclusions of NIST SRM 967 agreed with those of LN 24 except for the Beckman measurement system. CONCLUSIONS:Trueness investigation of routine creatinine assays on nine homogeneous systems demonstrates an encouraging outcome that meets clinical requirements. Among the nine homogeneous routine systems, Roche and Daiichi produce the most accurate results. The implementation of traceability is effective.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Liu Y,Xu GBsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
2364-9issue
17eissn
0366-6999issn
2542-5641journal_volume
123pub_type
杂志文章abstract:BACKGROUND:To date, there have been no reports on altered nitric oxide (NO) content in ischemia/reperfusion with regard to in vivo preconditioning procedures. These studies are important for understanding the mechanisms of NO during early myocardial ischemic preconditioning. The aim of the present study was to investig...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-07-05 00:00:00
abstract:BACKGROUND:Postoperative preablative stimulated thyroglobulin (ps-Tg) has been evaluated in predicting prognosis and success of ablation regarding differentiated thyroid cancer (DTC); however, its relationship with recurrence risk and radioiodine decision-making remains uncertain, especially in Chinese DTC patients. We...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.155086
更新日期:2015-04-20 00:00:00
abstract:BACKGROUND:The aim of this study was to investigate the potential relationship between the dynamic expression of Toll-like receptor 2 and 4 (TLR2/4) in peripheral blood mononuclear cells as well as changes in serum concentration of inflammatory factors and acute lung injury (ALI) in patients after orthotopic liver tran...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-04-20 00:00:00
abstract::198 preterm delivery placentas of 28 weeks to less than 37 weeks pregnancy were analyzed. Gross examination revealed that the differences of placenta previa and low implantation (2.91%), bipartate placenta and rupture of marginal sinus (0.97% respectively), large placenta (2.91%), short cord (4.85%), velamentous inser...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract:BACKGROUND:Spinal anesthesia was considered as a reasonable anesthetic option in severe preeclampsia when cesarean delivery is indicated, and there is no indwelling epidural catheter or contraindication to spinal anesthesia. However, the ideal dose of intrathecal bupivacaine has not been quantified for cesarean deliver...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:10.4103/0366-6999.150083
更新日期:2015-02-05 00:00:00
abstract::This paper reports the results of serological survey of 318,912 persons for nasopharyngeal carcinoma (NPC) in 21 cities and counties of south China. There were 8,441 persons with positive VCA-IgA antibody (single item positive) of EB virus (EBV), with a rate of 2.65%. In these VCA-IgA positive persons, 287 persons als...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:
更新日期:1995-04-01 00:00:00
abstract:BACKGROUND:Recent studies suggest that circulating DNA may be a potential tumor marker for lung cancer, but most of these studies are conducted between healthy controls and lung cancer patients, with few or no benign lung disease patients included. The objective of this study was to evaluate the performance of plasma D...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::Osteoarthritis (OA) models in bipedal rats were used to study the immune response, including the changes of IgG, antibody dependent cytotoxicity tests toward articular chondrocytes and the matrix. The results revealed significant difference between the bipedal and the quadruped rats. Immunological studies have proved ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract:Objective:Mechanical ventilation (MV) has long been used as a life-sustaining approach for several decades. However, researchers realized that MV not only brings benefits to patients but also cause lung injury if used improperly, which is termed as ventilator-induced lung injury (VILI). This review aimed to discuss the...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.4103/0366-6999.226840
更新日期:2018-05-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-06-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究,随机对照试验
doi:
更新日期:2008-03-20 00:00:00
abstract:BACKGROUND:OX40/OX40 ligand (OX40/OX40L) and programmed death-1/programmed death ligand-1 (PD-1/PD-L1) costimulatory signals play important roles in T cell-induced immune responses. The aim of this study was to investigate the roles of OX40/OX40L and PD-1/PD-L1 costimulatory pathways in mouse islet allograft rejection....
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Human urotensin II (UII) is the most potent mammalian vasoconstrictor identified so far. Our previous study showed that UII is a potent mitogen of airway smooth muscle cells (ASMC) inducing ASMC proliferation in a dose-dependent manner. The signal transduction pathway of UII mitogenic effect remains to be cl...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Hepatocellular carcinoma (HCC) ranked the second among the causes of cancer mortality in China since the 1990s. Up to now, medication still plays an important role in the treatment of HCC. The therapies based on the allicin as a potential chemopreventive analog although is in its infancy at the present time,...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-08-05 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and safety of misoprostol in term labour induction. DATA SOURCES:Data from published English and Chinese literatures about misoprostol in term labour induction were identified from Medline and CBMdisk (using the search terms "misoprostol" and "labour induction") before 2001; hand sea...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析,评审
doi:
更新日期:2004-03-01 00:00:00
abstract::Cogan’s syndrome (CS) is a rare inflammatory disorder characterized by interstitial keratitis and vestibuloauditory abnormalities often associated with various systemic manifestations. Involvement of cardiovascular system resembling systemic vasculitis may lead to severe complications and death. The present report des...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-06-05 00:00:00
abstract:BACKGROUND:Transradial approach catheterization is now widely used in coronary angiography and angioplasty. The ulnar artery, which is one of the two terminal branches of the brachial artery, may be a potential approach for cardiac catheterization. The aim of this study was to evaluate the safety and feasibility of a t...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:The Medtronic InSync Sentry is the first available cardiac resynchronization therapy defibrillator (CRT-D) which can monitor fluid status by measuring intrathoracic impedance. This study was designed to observe the effectiveness of intrathoracic impedance monitoring on detecting aggravation of heart failure....
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:The long-term safety and efficacy of drug-eluting stents (DES) versus bare metal stents (BMS) are unclear and controversial issues in patients with acute ST-elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). The purpose of this study was to compare the long-t...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-05-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.4103/0366-6999.241805
更新日期:2018-10-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2013-06-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-02-20 00:00:00
abstract:BACKGROUND:In many studies, obstructive sleep apnea (OSA) has been shown to be an independent risk factor for cardiovascular disease. Conversely, there are few reports establishing possible relation between OSA and venous thromboembolism (VTE). In this study, the aim is to evaluate OSA via polysomnography in patients w...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:Hypoxia-inducible factor 1 (HIF-1), a transcription factor, is overexpressed in common human cancers and their metastases. This study aimed at determining the expression levels of HIF-1alpha and vascular endothelial growth factor (VEGF) in SW480 cells and in colorectal adenocarcinoma tissue and ascertaining ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract:BACKGROUND:L-shaped incision of pronator quadratus (PQ) muscle along its radial and distal borders was always taken for distal radius fractures reduction and internal fixation. Repair of the PQ muscle was always recommended at the end of operation for some instructive reasons. But repair of PQ is not satisfied because ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) pneumonia is an important issue with significant morbidity and mortality in clinical practice, especially in diabetes mellitus (DM). Studies focusing on S. aureus pneumonia in DM is limited, we sought to make a relatively comprehensive exploration of clinica...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000270
更新日期:2019-06-20 00:00:00
abstract::Carbamyl phosphate synthetase I (CPS1) is an initial enzyme of urea synthetase system. It exists exclusively in liver cells and epithelial cells of the small intestine. By immunocytochemistry, 70.5% of 88 surgically resected gastric carcinomas (42 advanced and 46 early gastric carcinomas) was found to be CPS1 immunore...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract:BACKGROUND:It is the first multicenter clinical study in China to investigate zanamivir use among Chinese adolescents and adults with influenza-like illness (ILI) since 2009, when inhaled zanamivir (RELENZA(®)) was marketed in China. METHODS:An uncontrolled open-label, multicentre study to evaluate the antiviral activ...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:
更新日期:2012-09-01 00:00:00