Abstract：
:Dot-ELISA assay was compared with standard Sandwich ELISA in detecting parasite antigen in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same serum samples were used in both assays. With Dot-ELISA, 67 of 70 serum samples from microfilaremic patients were positive at a dilution of 1:50. End titers ranged from 1:80 to 1:1 280. While with Sandwich ELISA, 64 of the 70 serum samples were positive at a dilution of 1:10. End titers ranged from 1:10 to 1:320. The specificity of both assays was over 91%, but their sensitivity was markedly different. Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human serum, whereas the lower limit of detection by Sandwich-ELISA was 10 ng/ml parasite antigen. An additional advantage of Dot-ELISA is that it does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Zheng HJ,Fuhrman JA,Xu M,Cheng WF,Reddy MV,Piessens WFsubject
Has Abstractpub_date
1990-09-01 00:00:00pages
709-12issue
9eissn
0366-6999issn
2542-5641journal_volume
103pub_type
杂志文章abstract：OBJECTIVE:To describe the methods which were used to develop collagen-based materials for wound dressing. METHODS:Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：BACKGROUND:Early diagnosis and accurate staging are important to improve the cure rate and prognosis for pancreatic cancer. This study was performed to develop an automatic and accurate imaging processing technique system, allowing this system to read computed tomography (CT) images correctly and make diagnosis of panc...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000544
更新日期：2019-12-05 00:00:00
abstract：BACKGROUND:Transcatheter aortic valve implantation (TAVI) has emerged as the treatment choice for non-operable patients with severe symptomatic aortic stenosis (AS) and may be a good alternative to surgery for those at very high or prohibitive surgical risk. We performed a meta-analysis to evaluate the comparative bene...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析
doi：
更新日期：2013-03-01 00:00:00
abstract：:Injections of serotonin (5-HT) into the aortic arch of rats caused greater reductions of blood pressure (BP) and less increases in renal vascular resistance (RVR) than that into jugular veins, in contrast to norepinephrine (NE) which tended to cause larger increases in BP and RVR by the intra-aortic route. Indomethaci...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：BACKGROUND:Gastric intestinal metaplasia (GIM) is generally considered to be the main mucosal background for the development of gastric adenocarcinomas. Using linked color imaging (LCI), we noticed that the color pattern in areas of GIM was purple mixed with white on the epithelium with signs of mist that were detected...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000172
更新日期：2019-04-05 00:00:00
abstract：BACKGROUND:Chronic kidney disease (CKD) is a growing public health problem with well-established risk factors. Other contributing factors, however, remain to be identified. Systemic inflammation in asthma plays a significant role in the development of other diseases. We therefore initiated a study to assess whether the...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：BACKGROUND:Roundabout 5 (R5) is a monoclonal antibody which can neutralize the binding of Roundabout 1 (Robo1) to Slit2. Oral squamous cell carcinoma angiogenesis was significantly inhibited when R5 blocked slit-robo signaling pathway. However, the effect of R5 on the invasion of tongue cancer cells has not been invest...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To investigate the short- and long-term clinician and angiographic outcome of stenting or angioplasty for chronic total coronary artery occlusion. METHODS:A total of 95 patients with successful percutaneous transluminal coronary angioplasty (PTCA) for total occlusion coronary artery were identified out of 35...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:The long-term prognosis and causes of death of myocardial infarction (MI) in 62 diabetics were studied. The mean follow-up time was 6.2 years, 11 patients died in the acute stage of MI, 9 of them (81.8%) had anterior infarction and their major causes of death were ventricular fibrillation and cardiogenic shock (72.2%)...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of preoperative transcatheter arterial chemoembolization(TACE) on apoptosis of hepatocellular carcinoma (HCC) cells. METHODS:A total of 136 patients with HCC underwent liver resection. One to five courses of TACE prior to liver resection were performed in 79 patients (TACE group), in w...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-02-01 00:00:00
abstract：OBJECTIVE:To investigate the effectiveness of using continuous veno venous hemofiltration (CVVH) in the treatment of acute necrotizing pancreatitis (ANP). METHODS:Thirteen ANP patients were involved in this study, including 4 females and 9 males, averaging 50.6 +/- 10.8 years old. CT scans upon admission revealed 33% ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：OBJECTIVE:To study the expression of vascular endothelial growth factor (VEGF) in different parts of heart during early acute myocardial ischemia. METHODS:The expression of VEGF in control and study group in rats was performed with the Strept Avidin-Biotin-Peroxidase Complex (SABC) method. RESULTS:A rapid expression ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:Pulsed radiofrequency (PRF), as a non-invasive treatment of neuropathic pain (NP), has been widely administered clinically. Previous studies have shown that PRF has the potential to improve hyperalgesia in animal models of NP. However, there have been few reports to clarify whether the mechanism of PRF treat...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000619
更新日期：2020-01-20 00:00:00
abstract：BACKGROUND:Single incision laparoscopic colectomy has been performed in recent years, and has been shown to be feasible and safe. This study was to assess the feasibility of single incision laparoscopic right hemicolectomy and to compare the differences in different approaches. METHODS:This retrospective study include...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Liver cirrhosis is the fatal consequence of chronic hepatitis, making early diagnosis of liver cirrhosis critical. Liver biopsy is still the standard diagnostic method for liver cirrhosis, although its use in a broad population with alcoholism or hepatitis B virus (HBV) infection remains difficult. In this s...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-05-01 00:00:00
abstract：:Lectin binding patterns in psoriasis, lichen planus and seborrheic keratosis lesions were studied using 14 different biotinyl lectins and avidin-horseradish peroxidase. Compared with normal epidermis, there were significant quantitative and qualitative differences, some of which were characteristic. ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：BACKGROUND:Difficulties persist in differentiating pancreatic ductal adenocarcinomas (PDAC) from pancreatic inflammatory masses (PIM). Auxiliary diagnostic techniques which enhance the endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) diagnostic yield have been attempted, for example, K-ras mutation analysi...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Bone grafting is commonly used to repair bone defects. As the porosity of the graft scaffold increases, bone formation increases, but the strength decreases. Early attempts to engineer materials were not able to resolve this problem. In recent years, nanomaterials have demonstrated the unique ability to impr...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The persistence of anti-HBs was investigated in 95 juveniles who received plasma-derived HBV vaccine (vaccine group) and 63 counterparts who got anti-HBs from natural HBV infection (infection group) for a period of five years. The positive rates of anti-HBs from the first to fifth year in the vaccine group are 97.89%,...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：BACKGROUND:Graft-versus-host disease (GVHD) is a common complication of hematopoietic stem cell transplantation. Skin barrier disruption could induce thymic stromal lymphopoietin (TSLP) expression, and the expression of TSLP was increased in lesions of atopic dermatitis (AD)-like GVHD and lichen planus (LP)-like GVHD. ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.207475
更新日期：2017-06-20 00:00:00
abstract：BACKGROUND:Nucleolin (NCL) is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability, ribosome assembly, ribosomal RNA maturation, ribosomal DNA transcription, nucleocytoplasmic transport, and regulation of RNA stability and translation efficiency. In addition to ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.223853
更新日期：2018-02-05 00:00:00
abstract：BACKGROUND:Prostate specific membrane antigen (PSMA) can facilitate the growth, migration, and invasion of the LNCaP prostate cancer cell lines, but the underlying molecular mechanisms have not yet been clearly defined. Here, we investigated whether PSMA serves as a novel regulator of the phosphatidylinositol 3-kinase ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:The molecular mechanisms of Shenxianshengmai (SXSM), a traditional Chinese medicine, on bradycardia have been incompletely understood. The study tried to investigate the gene expression profile and proteomics of bradycardia rabbits' hearts after SXSM treatment. METHODS:Twenty-four adult rabbits were randoml...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.197999
更新日期：2017-01-20 00:00:00
abstract：Background:Pompe disease is a rare lysosomal glycogen storage disorder linked to the acid alpha-glucosidase gene (GAA). A wide clinical and genetic variability exists between patients from different ethnic populations, and the genotype-phenotype correlations are still not well understood. The aim of this study was to r...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.225056
更新日期：2018-02-20 00:00:00
abstract：BACKGROUND:Respiratory failure caused by metastatic pulmonary choriocarcinoma usually develops rapidly and is associated with a high mortality. The clinical management strategy is important in choriocarcinoma patients with acute respiratory failure. The objective of this study was to evaluate the clinical characteristi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：BACKGROUND:One stage transanal Soave pull-through procedure (TSPP) is a recent popular operation in the treatment of Hirschsprung's disease (HD). With no visible scar and a short hospital stay, it is well accepted by surgeons and mothers. In the conventional Soave procedure, a long rectal muscular cuff left for anocoli...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-01-05 00:00:00
abstract：BACKGROUND:In the era of highly active antiretroviral therapy (HAART), the use of antiretrovirals as post-exposure prophylaxis (PEP) was the most important strategy for preventing occupational exposure to blood or fluids containing human immunodeficiency virus (HIV). The objective of this study was to retrospectively e...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:In the past 5 years, new treatment materials and techniques offering a different concept in endovascular treatment have been described for cerebral arteriovenous malformations (CAVMs). The aim of this study was to assess the endovascular treatment of CAVMs by using a liquid embolic material, Onyx (Micro Ther...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-12-20 00:00:00
abstract：BACKGROUND:Alveolar echinococcosis (AE) is a disease in human and animals, and the cure rate is unsatisfactory. This study aimed to investigate the curative efficacy of different doses of locally applied radiotherapy on alveolar echinococcosis in rats. METHODS:Rats infected with Echinococcus multilocularis were random...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:In China, the prevalence of chronic kidney disease has increased significantly. Many studies shows that the spectrum of kidney disease had changed in recent years. We retrospectively analyzed the pathological types of renal biopsy and its spectrum change at the General Hospital of the Chinese People's Libera...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
