当前位置： SCI文献检索 > CHINESE MEDICAL JOURNAL期刊下所有文献 > Molecular Mechanisms of Increased Heart Rate in Shenxianshengmai-treated Bradycardia Rabbits.

Molecular Mechanisms of Increased Heart Rate in Shenxianshengmai-treated Bradycardia Rabbits.

Abstract：

BACKGROUND:The molecular mechanisms of Shenxianshengmai (SXSM), a traditional Chinese medicine, on bradycardia have been incompletely understood. The study tried to investigate the gene expression profile and proteomics of bradycardia rabbits' hearts after SXSM treatment. METHODS:Twenty-four adult rabbits were randomly assigned in four groups: sham, model, model plus SXSM treatment, and sham plus SXSM treatment groups. Heart rate was recorded in all rabbits. Then, total RNA of atria and proteins of ventricle were isolated and quantified, respectively. Gene expression profiling was conducted by gene expression chip, and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to confirm the results of gene expression chip. We used isobaric tags for elative and absolute quantitation and Western blotting to identify altered proteins after SXSM treatment. RESULTS:There was a constant decrease in the mean heart rate (32%, from 238 ± 6 beats/min to 149 ± 12 beats/min) after six weeks in model compared with that in sham group. This effect was partially reversed by 4-week SXSM treatment. Complementary DNA microarray demonstrated that the increased acetylcholinesterase and reduced nicotinic receptor were take responsibility for the increased heart rate. In addition, proteins involved in calcium handling and signaling were affected by SXSM treatment. Real-time RT-PCR verified the results from gene chip. Results from proteomics demonstrated that SXSM enhanced oxidative phosphorylation and tricarboxylic acid (TCA) cycle in ventricular myocardium to improve ATP generation. CONCLUSIONS:Long-term SXSM stimulates sympathetic transmission by increasing the expression of acetylcholinesterase and reduces the expression of nicotinic receptor to increase heart rate. SXSM also restored the calcium handling genes and altered genes involved in signaling. In addition, SXSM improves the ATP supply of ventricular myocardium by increasing proteins involved in TCA cycle and oxidation-respiratory chain.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Liu ZY,Huang J,Liu NN,Zheng M,Zhao T,Zhao BC,Wang YM,Pu JL

doi

10.4103/0366-6999.197999

subject

Has Abstract

pub_date

2017-01-20 00:00:00

pages

179-186

issue

2

eissn

0366-6999

issn

2542-5641

pii

ChinMedJ_2017_130_2_179_197999

journal_volume

130

pub_type

杂志文章

相关文献

CHINESE MEDICAL JOURNAL文献大全
  • Detection of siderotic nodules in the liver with susceptibility weighted imaging: correlations to serum ferritin, Child-Pugh grade and hyaluronic acid levels.

    abstract：BACKGROUND:Chronic liver disease causes aberrant formation of fibrous tissue that impedes normal liver function, ultimately resulting in liver cirrhosis. Iron uptake can occur within the hepatic parenchyma or within the various nodules that form in a cirrhotic liver, termed siderotic nodules (SN). We aimed to investiga...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Tao R,You ZL,Zhang JQ,Dai YM,Zhou DQ,Cai P,Fan Y,Cui JG,Wang J

    更新日期：2012-09-01 00:00:00

  • Establishment of multidrug-resistance cell line C(6)/adr and reversal of drug-resistance.

    abstract：OBJECTIVE:To investigate the mechanism of multidrug resistance (MDR) in a human glioma cell and methods for overcoming multi-drug resistance. METHODS:MDR cell line C(6)/adr was established. The expression of the mdr-1 gene and its P-glycoprotein (P-gp) in the C(6)/adr cell line was observed by RT-PCR and flow cytometr...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：10.3901/jme.2002.supp.238

    authors： Liu F,Wang C,Zhang Q,Li J,Zhang Y

    更新日期：2002-02-01 00:00:00

  • Study of microencapsulated pituitary transplantation. Preparation of the capsule and its property.

    abstract：:Microencapsules for pituitary transplantation was successfully developed with sodium alginate and poly-L-lysine (average molecular weight MW 70,000). The permeability of the capsular membrane (CM) was tested. The results showed that 60% of growth hormone (GH), thyroid stimulating hormone (TSH), prolactin (PRL) have pa...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Chen ZP,Bao YD

    更新日期：1994-03-01 00:00:00

  • Subclavian steal syndrome: report of 28 cases.

    abstract：OBJECTIVE:To study the experience in the treatment of subclavian steal syndrome (SSS) and the relation between SSS and the damage of brain. METHODS:In 28 patients with SSS, 23 were operated on. They complained of vertigo, TIA, and claudication of arms. One patient suffered from severe stem ischemia spasm. All the pati...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Guan H,Liu C,Yu Z

    更新日期：1999-11-01 00:00:00

  • Magnetic resonance imaging of the extraocular muscles and corresponding cranial nerves in patients with special forms of strabismus.

    abstract：BACKGROUND:With the technical advances, magnetic resonance imaging (MRI) is now sensitive enough to detect subtle structural abnormalities of ocular motor nerves arising from the brainstem and orbits of living subjects. This study was designed to delineate the MRI characteristics in patients with special forms of strab...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Jiao YH,Zhao KX,Wang ZC,Qian XH,Wu X,Man FY,Lu W,She HC

    更新日期：2009-12-20 00:00:00

  • Nanoparticles as a vaccine adjuvant of anti-idiotypic antibody against schistosomiasis.

    abstract：BACKGROUND:The development of new adjuvants for human use has been the focus of attention. This study's aim is to explore the possibility of using nanoparticle Ca nanoparticles (CA) as a vaccine adjuvant of anti-idiotypic antibody NP30 against schistosomiasis and its protective mechanisms. METHODS:Nanoparticle CA-NP30...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Feng ZQ,Zhong SG,Li YH,Li YQ,Qiu ZN,Wang ZM,Li J,Dong L,Guan XH

    更新日期：2004-01-01 00:00:00

  • Outcome of surgery for sinus of Valsalva aneurysm with discrete membranous subaortic stenosis.

    abstract：BACKGROUND:Sinus of Valsalva aneurysm (SVA) is a rare cardiac anomaly, and SVA with discrete membranous subaortic stenosis is even rarer. The aim of the study was to make sure the incidence of SVA with discrete membraneous subaortic stenosis in SVA and their surgical results. We retrospectively analyzed 234 patients re...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Guo HW,Chang Q,Yu CT,Sun XG,Qian XY,Hu SS

    更新日期：2012-05-01 00:00:00

  • Transplantation of neonatal cardiomyocytes plus fibrin sealant restores myocardial function in a rat model of myocardial infarction.

    abstract：BACKGROUND:Most cardiac regenerative approaches can restore injured heart muscles. In this study, we investigated if fibrin sealant could help neonatal cardiomyocytes restore myocardial function in a rat model of myocardial infarction. METHODS:The left anterior descending artery in adult female Sprague-Dawley (SD) rat...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Li YS,Gao BR

    更新日期：2007-11-20 00:00:00

  • Enzyme-linked immunoelectrotransfer blotting analysis of human serologic responses to infective hookworm larval antigen.

    abstract：OBJECTIVE:To explore the possibility of using specific antigens for immunodiagnosis of hookworm disease in endemic area. METHOD:Infective third-stage larvae of the canine hookworm, Ancylostoma caninum (A. caninum), were prepared as the source of antigen. Enzyme-linked immunoelectrotransfer blotting (EITB) was employed...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Xue H,Liu S,Ren H,Qiang H,Xiao S,Feng Z,Hotez P

    更新日期：1999-03-01 00:00:00

  • Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection.

    abstract：BACKGROUND:Acinetobacter baumanii (A. baumanii ) remains an important microbial pathogen resulting in nosocomial acquired infections with significant morbidity and mortality. The mechanism by which nosocomial bacteria, like A. baumanii, attain multidrug resistance to antibiotics is of considerable interest. The aim in ...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Dai N,Li DZ,Chen JC,Chen YS,Geng R,Hu YH,Yang JP,DU J,Hu CP,Zhang W,Li JS,Yu Q,Wan HY,Mu L,Zhong XN,Wei LP,Ma JJ,Wang QY,Hu K,Tian GZ,Cai SX,Wang RQ,He B,Wang SQ,Wang ZW,Zhao SR,Gao ZC

    更新日期：2010-09-01 00:00:00

  • The basic clinical diagnostic framework synergized.

    abstract：OBJECTIVE:To develop a diagnostic framework that would help defining clinical problems by the expanded understanding with traditional Chinese medicine (C Med) and western medicine (W Med). METHODS:The basic attributes of diagnosis and the use of diagnostic techniques of W Med and C Med are reviewed. Some of the variou...

    journal_title：Chinese medical journal

    pub_type： 杂志文章,评审

    doi：

    authors： Yu EC

    更新日期：1998-05-01 00:00:00

  • Induction of apoptosis by homoharringtonine in G1 phase human chronic myeloid leukemic cells.

    abstract：BACKGROUND:Homoharringtonine (HHT) is a cephalotaxine ester derived from an evergreen tree found wildely throughout southern China, which has antileukemic activities against a variety of acute myeloid leukemic cells. For the sake of illustrating the mechanisms of HHT in the treatment of leukemia, we assessed the effect...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Mai WY,Lin MF

    更新日期：2005-03-20 00:00:00

  • Fungal infection in organ transplant patients.

    abstract：PURPOSE:To review the characteristics and evolution of the fungal spectrum, and the risk factors causing fungal infection, and to make progress in diagnosing fungal infection after organ transplantation. DATA SOURCES:An English-language literature search (MEDLINE 1990 - 2000) and bibliographic review of textbooks and ...

    journal_title：Chinese medical journal

    pub_type： 杂志文章,评审

    doi：

    authors： Hong W,Wen H,Liao W

    更新日期：2003-09-01 00:00:00

  • A multi-center, double-blind, randomized, parallel group study to evaluate the effects of two different doses of losartan on morbidity and mortality in Chinese patients with symptomatic heart failure intolerant of angiotensin converting enzyme inhibitor t

    abstract：BACKGROUND:There have been no mortality/morbidity endpoint studies with losartan in Chinese heart failure patients. The objective was to evaluate the effects of high-dose vs. low-dose losartan on clinical outcomes in Chinese subjects with heart failure. METHODS:This study was a post hoc analysis of the Heart failure E...

    journal_title：Chinese medical journal

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：

    authors： Hu DY,Huang J,Cai NS,Zhu WL,Li YS,Massaad R,Hanson ME,Dickstein K

    更新日期：2012-11-01 00:00:00

  • Thirty-day Postoperative Complications following Primary Total Knee Arthroplasty: A Retrospective Study of Incidence and Risk Factors at a Single Center in China.

    abstract：BACKGROUND:Total knee arthroplasty (TKA) may be associated with serious complications that adversely affect outcomes and increase the likelihood of disability. However, few studies with sufficient sample size have reported postoperative complications following TKA among Chinese patients. This study aimed to evaluate co...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：10.4103/0366-6999.213071

    authors： Feng B,Lin J,Jin J,Qian WW,Wang W,Weng XS

    更新日期：2017-11-05 00:00:00

  • Effect of metoprolol on sarcoplasmic reticulum Ca2+ leak in a rabbit model of heart failure.

    abstract：BACKGROUND:Studies have shown that β-blockers can improve cardiac performance in heart failure (HF) by reversing protein kinase A (PKA)-mediated sarcoplasmic reticulum (SR) Ca2+ leak. However, it is being strongly questioned as to whether the PKA-mediated ryanodine receptor (RyR2) hyper-phosphorylation is a critical re...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Zhang SJ,Liu ZH,Zou C,Wang L

    更新日期：2012-03-01 00:00:00

  • Elective percutaneous intervention for unprotected left main coronary artery stenosis complicated with left anterior descending artery chronic total occlusive lesions.

    abstract：BACKGROUND:The patients with unprotected left main coronary artery (ULMCA) stenosis and chronic total occlusion (CTO) lesions at the left anterior descending (LAD) artery are often recommended for bypass surgery. However, some of these patients are deemed inoperable or are at high risk for surgery. In this study, we ex...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Yang GT,Han YL,Jing QM,Wang SL,Li Y,Ma YY,Wang G,Luan B,Wang XZ,Wang B,Wang ZL,Wang DM

    更新日期：2010-04-05 00:00:00

  • Expression of vascular endothelial growth factor (VEGF) and VEGF-C in serum and tissue of Wilms tumor.

    abstract：BACKGROUND:Angiogenesis and lymphogenesis which were promoted by vascular endothelial growth factor (VEGF) and VEGF-C are important in the growth and metastasis of solid tumors. The high level of VEGF and VEGF-C were distributed in numerous types of cancers, but their distribution and expression in Wilms tumor, the mos...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Wang L,Zhang D,Chen XR,Fan YX,Wang JX

    更新日期：2011-11-01 00:00:00

  • Death mode-dependent reduction in succinate dehydrogenase activity in hair cells of aging rat cochleae.

    abstract：BACKGROUND:Our previous studies have shown that both apoptosis and necrosis are involved in hair cell (HC) pathogenesis in aging cochleae. To better understand the biological mechanisms responsible for the regulation of HC death, we examined the activity of succinate dehydrogenase (SDH), a mitochondrial bioenergetic en...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Yang WP,Hu BH,Sun JH,Zhai SQ,Henderson D

    更新日期：2010-07-01 00:00:00

  • Baseline Naive CD4+ T-cell Level Predicting Immune Reconstitution in Treated HIV-infected Late Presenters.

    abstract：BACKGROUND:Among HIV-infected patients initiating antiretroviral therapy (ART), early changes in CD4+ T-cell subsets are well described. However, HIV-infected late presenters initiating treatment present with a suboptimal CD4+ T-cell reconstitution and remain at a higher risk for AIDS and non-AIDS events. Therefore, fa...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：10.4103/0366-6999.193460

    authors： Guo FP,Li YJ,Qiu ZF,Lv W,Han Y,Xie J,Li YL,Song XJ,Du SS,Mehraj V,Li TS,Routy JP

    更新日期：2016-11-20 00:00:00

  • Applicability of prostaglandin E1 indirect portal vein angiography in patients with portal hypertension.

    abstract：:The imaging quality of the portal vein was obviously improved with prostaglandin E1 (PGE1) indirect portal vein digital subtraction angiography (DSA) in 23 cases. The time-density curve showed that the occurrence rate of opposite hepatic blood flow of splenic vein (SV) was the highest (17.4%). The total visualization ...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Peng Z,Dai Z,Qiao S

    更新日期：1995-05-01 00:00:00

  • Comparison of Dot-ELISA with Sandwich ELISA in detecting circulating antigen in patients with bancroftian filariasis.

    abstract：:Dot-ELISA assay was compared with standard Sandwich ELISA in detecting parasite antigen in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same serum samples were used in both assays. With Dot-ELISA, 67 of 70 serum samples from microfilaremic patients were positive at a dilution of...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Zheng HJ,Fuhrman JA,Xu M,Cheng WF,Reddy MV,Piessens WF

    更新日期：1990-09-01 00:00:00

  • Mechanisms of selective head cooling for resuscitating damaged neurons during post-ischemic reperfusion.

    abstract：OBJECTIVE:To evaluate the efficacy and the mechanism of application of selective head cooling on neuronal morphological damage during postischemic reperfusion in a rabbit model. METHODS:168 New Zealand rabbits were randomized into three groups. Group I [n = 24, (38 +/- 0.5) degrees C, non-ischemic control]; Group II [...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Duan M,Li D,Xu J

    更新日期：2002-01-01 00:00:00

  • Genetic analysis of a congenital nephrogenic diabetes insipidus pedigree.

    abstract：BACKGROUND:As an X-linked recessive way, arginine vasopressin receptor 2 (AVPR2) gene mutation resulted in a hereditary disease - congenital nephrogenic diabetes insipidus (CNDI). We found a suspect clinical CNDI pedigree. In order to identify the genetic etiology, we performed the genetic analysis. METHODS:The clinic...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Shen Y,Lai X,Xiao X,Li J,Yu R,Gao H,Zhang M

    更新日期：2014-01-01 00:00:00

  • An epidemic of encephalitis and meningoencephalitis in children caused by echovirus type 30 in Shanghai.

    abstract：:An outbreak of viral central nervous system infection occurred from May to September, 1991 in Shanghai, China. Totally 376 cases were admitted into our hospital, 54% with encephalitis and 46% with meningoencephalitis. Echovirus type 30 was isolated from CSF and stools of the patients. Only 3 of the patients were under...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Wang DM,Zhao GC,Zhuang SM,Zhang YC

    更新日期：1993-10-01 00:00:00

  • Relationship between the expression of Toll-like receptor 2 and 4 in mononuclear cells and postoperative acute lung injury in orthotopic liver transplantation.

    abstract：BACKGROUND:The aim of this study was to investigate the potential relationship between the dynamic expression of Toll-like receptor 2 and 4 (TLR2/4) in peripheral blood mononuclear cells as well as changes in serum concentration of inflammatory factors and acute lung injury (ALI) in patients after orthotopic liver tran...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Chi XJ,Cai J,Luo CF,Cheng N,Hei ZQ,Li SR,Luo GJ

    更新日期：2009-04-20 00:00:00

  • Correlation between retinal fluorescein angiography and blood viscosity and other factors in patients with primary open angle glaucoma.

    abstract：OBJECTIVE:To investigate the correlation among retinal fluorescein angiography, blood viscosity, and other factors in patients with open angle glaucoma (POAG). METHODS:Multiple step regression analysis was made to investigate the correlation between each of the following blood vessel filling times: the arm-choroid (A-...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Liu X,Zhou W,Ye T,Ge J,Cai X,Cheng A,Lin Y,Liang L,Tang H,Chen Z

    更新日期：1997-09-01 00:00:00

  • Multimodality imaging assessments of response to metformin therapy for breast cancer in nude mice.

    abstract：BACKGROUND:Metformin is the most widely used anti-diabetic drug in the world. An increasing body of evidence shows metformin also blocks cell cycle progression and selectively induces apoptosis via caspase activation in some breast tumor cells. Diffusion-weighted imaging (DWI) and bioluminescence imaging (BLI) have gre...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Mao Y,Xia R,Wang L,Wang YQ,Gao FB

    更新日期：2013-01-01 00:00:00

  • Long non-coding RNAs expression profile in HepG2 cells reveals the potential role of long non-coding RNAs in the cholesterol metabolism.

    abstract：BACKGROUND:Green tea has been shown to improve cholesterol metabolism in animal studies, but the molecular mechanisms underlying this function have not been fully understood. Long non-coding RNAs (lncRNAs) have recently emerged as a major class of regulatory molecules involved in a broad range of biological processes a...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：10.4103/0366-6999.147824

    authors： Liu G,Zheng X,Xu Y,Lu J,Chen J,Huang X

    更新日期：2015-01-05 00:00:00

  • Chinese patients with transient ischemic attacks.

    abstract：:In 123 cases of transient ischemic attacks, 72 were followed up for 22 years. The percentage of complete stroke was 46.5% and the fatality rate 47.9%. Causes of death, survival rate by life table, limitation of indication of neurovascular surgical operation and rehabilitation of survived patients were studied. The cau...

    journal_title：Chinese medical journal

    pub_type： 杂志文章

    doi：

    authors： Wang XD

    更新日期：1990-09-01 00:00:00