MicroRNA Profiling of Transgenic Mice with Myocardial Overexpression of Nucleolin.

Abstract:

BACKGROUND:Nucleolin (NCL) is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability, ribosome assembly, ribosomal RNA maturation, ribosomal DNA transcription, nucleocytoplasmic transport, and regulation of RNA stability and translation efficiency. In addition to its anti-apoptotic properties, the underlying mechanisms associated with NCL-related roles in different cellular processes remain unclear. In this study, the effect of NCL on microRNA (miRNA) expression was evaluated by generating transgenic mice with myocardial overexpression of NCL and by analyzing microarrays of mature and precursor miRNAs from mice. METHODS:Using microinjection of alpha-MyHc clone 26-NCL plasmids, we generated transgenic mice with myocardial overexpression of NCL firstly, and then mature and precursor miRNAs expression profiles were analyzed in NCL transgenic mice (n = 3) and wild-type (WT) mice (n = 3) by miRNA microarrays. Statistical Package for the Social Sciences version 16.0 software (SPSS, Inc., Chicago, IL, USA) was used to perform Student's t-test, and statistical significance was determined at P < 0.05. RESULTS:Several miRNAs were found to be differentially expressed, of which 11 were upregulated and 4 were downregulated in transgenic mice with myocardial overexpression of NCL compared to those in WT mice. Several differentially expressed miRNAs were subsequently confirmed and quantified by real-time quantitative reverse transcription-polymerase chain reaction. Bioinformatics analysis was used for the prediction of miRNA targets. Furthermore, in vitro experiments showed that NCL regulated miR-21 expression following hydrogen peroxide preconditioning. CONCLUSIONS:Myocardial-protection mechanisms exerted by NCL might be mediated by the miRNAs identified in this study.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Lyu QL,Jiang BM,Zhou B,Sun L,Tong ZY,Li YB,Tang YT,Sun H,Liu MD,Xiao XZ

doi

10.4103/0366-6999.223853

subject

Has Abstract

pub_date

2018-02-05 00:00:00

pages

339-346

issue

3

eissn

0366-6999

issn

2542-5641

pii

ChinMedJ_2018_131_3_339_223853

journal_volume

131

pub_type

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