Abstract:
:The identification of individual dog chromosomes is problematic because the 38 pairs of autosomes are small and acrocentric. Here we describe the design and application of a FISH tool that enables definitive identification of each dog autosome in a normal karyotype, without relying on subjective interpretation of DAPI banding patterns. From a high-resolution physical map of the canine genome, we have chosen a panel of 80 canine chromosome-specific BAC clones. DNA from each clone is labeled with one of five different fluorochrome-conjugated nucleotides. By selecting one to three spatially separated BACs per chromosome, and labelling them with a distinctive combination of colours, each autosome can be identified objectively and orientated accurately, irrespective of the quality of DAPI chromosome banding. This tool, or part of it, can be used for any purpose where accurate identification of canine autosomes in a normal karyotype is essential. In this study, we demonstrate use of the 'colour code' for chromosome identification following CGH analysis of unbalanced genomic aberrations in a canine brain tumour. Our method is an improvement of an earlier procedure, featuring chromosome-specific BACs and sequential FISH hybridisations, as it enables simultaneous identification of all chromosomes in a single hybridisation.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Courtay-Cahen C,Griffiths LA,Hudson R,Starkey Mdoi
10.1159/000098187subject
Has Abstractpub_date
2007-01-01 00:00:00pages
198-204issue
3eissn
1424-8581issn
1424-859Xpii
000098187journal_volume
116pub_type
杂志文章abstract::In recent years increasing attention has been paid to the cytogenetic control of Italian Mediterranean river buffalo (BBU) bulls authorized as sires which are registered in the stud book. Chromosome abnormalities described in this species are mainly numerical and affecting sex chromosomes. During routine cytogenetic a...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000342360
更新日期:2013-01-01 00:00:00
abstract::We analyzed genetic changes in condylomas (four cases), vulvar intraepithelial neoplasia I-III (VIN I-III, eleven cases), and primary vulvar squamous cell carcinomas (VSCC, ten cases) by high-resolution comparative genomic hybridization (HR-CGH) and flowcytometry. All samples were also human papilloma virus (HPV)-geno...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078559
更新日期:2004-01-01 00:00:00
abstract::In order to understand the mechanisms of formation of chromosomal aberrations, studies performed on human syndromes with genomic instability can be fruitful. In this report, the results from studies in our laboratory on the importance of the transcription-coupled repair (TCR) pathway on the induction of chromosomal da...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000077471
更新日期:2004-01-01 00:00:00
abstract::Using FISH and RH mapping a chromosomal map of rat chromosome 10 (RNO10) was constructed. Our mapping data were complemented by other published data and the final map was compared to maps of mouse and human chromosomes. RNO10 contained segments homologous to mouse chromosomes (MMU) 11, 16 and 17, with evolutionary bre...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000063052
更新日期:2002-01-01 00:00:00
abstract::The Hodgkin cell line U-HO1 was established from a malignant pleural effusion of a 23-year-old male patient during the end stage of refractory nodular sclerosing classical Hodgkin lymphoma (cHL). Since its establishment in 2005, U-HO1 has maintained stable characteristics in vitro and has a doubling time of about 4 da...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000112062
更新日期:2007-01-01 00:00:00
abstract::Stripe rust (Puccinia striiformis tritici (Pst)) is one of the most destructive diseases of wheat in the world. Exploiting and utilizing stripe rust resistance genes of wild species has become an essential strategy for resistance breeding. Psathyrostachyshuashanica Keng ex Kuo is a wild species in Triticeae that has b...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000324928
更新日期:2011-01-01 00:00:00
abstract::High density livestock to human comparative maps are necessary for the implementation of comparative positional candidate gene cloning. We have constructed a high-density comparative radiation hybrid (RH) map of the telomeric end of bovine chromosome 2 (BTA2) using a 12,000-rad whole genome cattle-hamster radiation hy...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076295
更新日期:2003-01-01 00:00:00
abstract::The human brain is distinguished by its remarkable size, high energy consumption, and cognitive abilities compared to all other mammals and non-human primates. However, little is known about what has accelerated brain evolution in the human lineage. One possible explanation is that the appearance of advanced communica...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000335465
更新日期:2012-01-01 00:00:00
abstract::Merle is a distinct coat color and pattern found in numerous species, including the domestic dog, characterized by patches of diluted eumelanin (black pigment) interspersed among areas of normal pigmentation. In dogs, this variegated pattern is caused by an insertion of a SINE element into the canine PMEL gene. Althou...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000491408
更新日期:2018-08-03 00:00:00
abstract::Based on the analysis of 20 different monocot and eudicot species, we propose that the centromeric distribution of the phosphorylated histone H2AThr120 is evolutionary highly conserved across species with mono- and holocentric chromosomes. Therefore, antibodies recognizing the phosphorylated threonine 120 of the histo...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000360018
更新日期:2014-01-01 00:00:00
abstract::Holoprosencephaly (HPE) is the most common congenital malformation of the brain and face in humans. In this study we report the analysis of SIL (Sumacr;CL iumacr;nterrupting lumacr;ocus) as a candidate gene for HPE. Fluorescent in situ hybridization (FISH) analysis using a BAC 246e16 confirmed the assignment of SIL to...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000064057
更新日期:2002-01-01 00:00:00
abstract::Three activities hallmark meiotic cell division: homologous chromosome pairing, synapsis, and recombination. Recombination and synapsis are well-studied but homologous pairing still holds many black boxes. In the past several years, many studies in plants have yielded insights into the mechanisms of chromosome pairing...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000121080
更新日期:2008-01-01 00:00:00
abstract::Under specific stress treatments, the microspore can be induced in vitro to deviate from its gametophytic development and to reprogram towards embryogenesis, becoming a totipotent cell and forming haploid embryos. These can further regenerate homozygous plants for production of new isogenic lines, an important biotech...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000365232
更新日期:2014-01-01 00:00:00
abstract::DNA methylation has been implicated in mammalian development. Transcription units contain CpG islands, but expression of CpG island associated genes in normal tissues was not believed to be controlled by DNA methylation. There are, however, numerous CpG islands containing tissue-dependent and differentially methylated...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078205
更新日期:2004-01-01 00:00:00
abstract::Since the discovery of SRY/SRY as a testis-determining gene on the mammalian Y chromosome in 1990, extensive studies have been carried out on the immediate target of SRY/SRY and genes functioning in the course of testis development. Comparative studies in non-mammalian vertebrates including birds have failed to find a...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000071599
更新日期:2002-01-01 00:00:00
abstract::Genetic strategies for the post-genomic sequence age will be designed to provide information about gene function in a myriad of physiological processes. Here an ENU mutagenesis program (http://reprogenomics.jax.org) is described that is generating a large resource of mutant mouse models of infertility; male and female...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078192
更新日期:2004-01-01 00:00:00
abstract::Here, a new satellite-DNA family is isolated and characterized from wedge sole, Dicologoglossa cuneata Moreau, 1881 (Pleuronectiformes), a fish having a small genome. This satellite-DNA family of sequences was isolated by conventional cloning after digestion of genomic DNA with the DraI restriction enzyme. Repeat unit...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000138896
更新日期:2008-01-01 00:00:00
abstract::The relationship between the apparently random chromosomal changes found in aneuploidy and the genetic instability driving the progression of cancer is not clear. We report a test of the hypothesis that aneuploid chromosomal abnormalities might be selected to preserve cell-survival genes during loss of heterozygosity ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000100406
更新日期:2007-01-01 00:00:00
abstract::RNA interference (RNAi) refers to the selective degradation of mRNA induced by double-stranded RNA (dsRNA), first discovered in Caenorhabditis elegans. Homology-dependent silencing phenomena related to RNAi have been observed in many species from all eukaryotic kingdoms. RNAi and related mechanisms share several conse...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000078215
更新日期:2004-01-01 00:00:00
abstract::An integrated genetic linkage map was developed for the turkey (Meleagris gallopavo) that combines the genetic markers from the three previous mapping efforts. The UMN integrated map includes 613 loci arranged into 41 linkage groups. An additional 105 markers are tentatively placed within linkage groups based on two-p...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000109627
更新日期:2007-01-01 00:00:00
abstract::Genomic imprinting has a special role in placental biology. Imprinted genes are often strongly expressed in the placenta, and the allelic expression bias due to imprinting is sometimes stronger in this extraembryonic organ than in the embryo and adult. Mutations, epimutations, and uniparental disomies affecting imprin...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000090842
更新日期:2006-01-01 00:00:00
abstract::The detection of quantitative changes in genomic DNA, i.e. deletions and duplications or Copy Number Variants (CNVs), has recently gained considerable interest. First, detailed analysis of the human genome showed a surprising amount of CNVs, involving thousands of genes. Second, it was realised that the detection of C...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000184723
更新日期:2008-01-01 00:00:00
abstract::Breast cancer is a complex and heterogeneous disease, and the establishment of cell models in order to properly study the disease at the molecular and cellular level is of utmost importance. Here, we present the cytogenetic characterization and gene expression analysis of the tumoral mammary rat cell line CLS-ACI-1. T...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000441374
更新日期:2015-01-01 00:00:00
abstract::Neacomys (Sigmodontinae) comprises 8 species mainly found in the Amazonian region. We describe 5 new karyotypes from Brazilian Amazonia: 2 cytotypes for N. paracou (2n = 56/FNa = 62-66), 1 for N. dubosti (2n = 64/FNa = 68), and 2 for Neacomys sp. (2n = 58/FNa = 64-70), with differences in the 18S rDNA. Telomeric probe...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000441173
更新日期:2015-01-01 00:00:00
abstract::A 5-year-old river buffalo cow underwent cytogenetic investigation since it had only one male offspring, apparently with normal body constitution, which died one month after birth. The female carrier had normal body conformation and internal sex adducts, as revealed by rectal palpation performed by a specialist veteri...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000318011
更新日期:2011-01-01 00:00:00
abstract::Mismatch repair (MMR) systems are central to maintaining genome stability in prokaryotes and eukaryotes. MMR proteins play a fundamental role in avoiding mutations, primarily by removing misincorporation errors that occur during DNA replication. MMR proteins also act during genetic recombination in steps that include ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000080593
更新日期:2004-01-01 00:00:00
abstract::Correct imprinting is crucial for normal fetal and placental development in mammals. Experimental evidence in animal models and epidemiological studies in humans suggest that assisted reproductive technologies (ARTs) can interfere with imprinted gene regulation in gametogenesis and early embryogenesis. Bos taurus is a...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000322627
更新日期:2011-01-01 00:00:00
abstract::The karyotype of the pine woods treefrog, Hyla femoralis, is characterized by primitive XY female/XX male sex chromosomes. The sole difference between the X and the Y is the presence of a nucleolus organizer region (NOR) in the X. Due to a deletion of the NOR in the Y, this chromosome is distinctly smaller than the X....
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000073421
更新日期:2003-01-01 00:00:00
abstract::B chromosomes are now known in eight Brazilian rodent species: Akodon montensis, Holochilus brasiliensis, Nectomys rattus, N. squamipes, Oligoryzomys flavescens, Oryzomys angouya, Proechimys sp. 2 and Trinomys iheringi. Typically these chromosomes are heterogeneous relative to size, morphology, banding patterns, prese...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000079296
更新日期:2004-01-01 00:00:00
abstract::It is widely accepted that the H2AX histone in its phosphorylated form (gamma-H2AX) is related to the repair of DNA double-strand breaks (DSBs). In several organisms, gamma-H2AX presence has been demonstrated in meiotic processes such as recombination and sex chromosome inactivation during prophase I (from leptotene t...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000100416
更新日期:2007-01-01 00:00:00