Cytogenetic Assessment of the Rat Cell Line CLS-ACI-1: An in vitro Cell Model for Mycn Overexpression.

Abstract:

:Breast cancer is a complex and heterogeneous disease, and the establishment of cell models in order to properly study the disease at the molecular and cellular level is of utmost importance. Here, we present the cytogenetic characterization and gene expression analysis of the tumoral mammary rat cell line CLS-ACI-1. The use of banding and molecular cytogenetic techniques allowed the description of the complex CLS-ACI-1 karyotype and the identification of breakpoints in clonal chromosome rearrangements. Moreover, a Mycn and Erbb2 comparative expression analysis by RT-qPCR was performed, revealing a high expression level of Mycn in CLS-ACI-1 cells. Moreover, a considerable number of putative mutated genes and chromosome alterations detected through cytogenetic analysis seem to be in the MYCN biological network. Therefore, the CLS-ACI-1 cell line is presented as a promising cell model for the study of the role of MYCN in breast cancer and also as a tool for developing appropriate cancer therapies, namely for Mycn targeting.

journal_name

Cytogenet Genome Res

authors

Meles S,Adega F,Castro J,Chaves R

doi

10.1159/000441374

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

285-95

issue

4

eissn

1424-8581

issn

1424-859X

pii

000441374

journal_volume

146

pub_type

杂志文章
  • In silico comparison of gene expression levels in ten human tumor types reveals candidate genes associated with carcinogenesis.

    abstract::Most human cancers are characterized by genomic instability. Changes associated with such may result in altered expression of numerous genes. The sequence information available in the public databases can be used to identify transcripts differentially expressed in cancers. Determining cancer-related genes that are com...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000087513

    authors: Chen S,Zhu B,Yu L

    更新日期:2006-01-01 00:00:00

  • Fragile sites in domestic animal chromosomes: molecular insights and challenges.

    abstract::Fragile sites are intriguing cytogenetic phenomena that have been extensively investigated in human and laboratory animal chromosomes over the past 40 years, but domestic animal species have been studied sporadically. Interest in the field has been recently renewed as increasing numbers of fragile site regions are clo...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000245910

    authors: Riggs PK,Rønne M

    更新日期:2009-01-01 00:00:00

  • Analysis using sperm-FISH of a putative interchromosomal effect in boars carrying reciprocal translocations.

    abstract::The occurrence of interchromosomal effects (ICE) in reciprocal translocation carriers still remains contradictory in the human literature. We used the pig as an animal model to investigate whether the structure of the reciprocal translocations as well as the size and/or type of the chromosomes not involved in the rear...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000245920

    authors: Bonnet-Garnier A,Guardia S,Pinton A,Ducos A,Yerle M

    更新日期:2009-01-01 00:00:00

  • A high incidence of adjacent-1 meiotic segregation pattern, revealed by multicolor sperm FISH, in a carrier boar of a new reciprocal translocation t(6;16)(p13;q23).

    abstract::Reciprocal translocations pose a serious problem in pig breeding due to the reduced fertility of the carriers. This paper presents a new reciprocal translocation in a phenotypically normal, but hypoprolific (20% reduction) boar. Chromosome banding as well as the FISH technique with the use of BAC and telomeric probes ...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000355213

    authors: Kociucka B,Szczerbal I,Bugaj S,Orsztynowicz M,Switonski M

    更新日期:2014-01-01 00:00:00

  • Differential loss of expression of common fragile site genes between oral tongue and oropharyngeal squamous cell carcinomas.

    abstract::The common fragile sites (CFSs) are large regions of profound genomic instability found in all individuals. A number of the CFSs have been found to span genes that extend over large genomic regions (>700 kb). The expression of these genes is frequently abrogated in a number of different cancers and several of them hav...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000138886

    authors: Soderberg C,Perez DS,Ukpo OC,Liang X,O'Reilly AG,Moore EJ,Kademani D,Smith DI

    更新日期:2008-01-01 00:00:00

  • Molecular and clinical characterization of de novo and familial cases with microduplication 3q29: guidelines for copy number variation case reporting.

    abstract::Microdeletions of 3q29 have previously been reported, but the postulated reciprocal microduplication has only recently been observed. Here, cases from four families, two ascertained in Toronto (Canada) and one each from Edinburgh (UK) and Leiden (Netherlands), carrying microduplications of 3q29 are presented. These fa...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000184693

    authors: Goobie S,Knijnenburg J,Fitzpatrick D,Sharkey FH,Lionel AC,Marshall CR,Azam T,Shago M,Chong K,Mendoza-Londono R,den Hollander NS,Ruivenkamp C,Maher E,Tanke HJ,Szuhai K,Wintle RF,Scherer SW

    更新日期:2008-01-01 00:00:00

  • Towards many colors in FISH on 3D-preserved interphase nuclei.

    abstract::The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor la...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章,评审

    doi:10.1159/000094227

    authors: Walter J,Joffe B,Bolzer A,Albiez H,Benedetti PA,Müller S,Speicher MR,Cremer T,Cremer M,Solovei I

    更新日期:2006-01-01 00:00:00

  • Fixed and unstable I-related transposable elements in heterochromatin of Drosophila melanogaster.

    abstract::Transposable elements are disproportionately abundant in the heterochromatin of Drosophila melanogaster. Among the forces contributing to this bias in genomic distribution, fixation due to positive selection has been put forward. We have studied I-related elements which are located in pericentromeric heterochromatin a...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000084950

    authors: Junakovic N,Fortunati D,Soriano S

    更新日期:2005-01-01 00:00:00

  • Activation of rye 5RL neocentromere by an organophosphate pesticide.

    abstract::An interstitial constriction located on the long arm of rye chromosome 5R (5RL) shows neocentromeric activity at meiosis. In some meiocytes this region is strongly stretched orienting with the true centromere to opposite poles at metaphase I, and keeping sister chromatid cohesion at anaphase I. We found previously tha...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000325744

    authors: Cuacos M,González-García M,González-Sánchez M,Puertas MJ,Vega JM

    更新日期:2011-01-01 00:00:00

  • Microhomology-mediated microduplication in the y chromosomal azoospermia factor a region in a male with mild asthenozoospermia.

    abstract::Y chromosomal azoospermia factor (AZF) regions AZFa, AZFb and AZFc represent hotspots for copy number variations (CNVs) in the human genome; yet the number of reports of AZFa-linked duplications remains limited. Nonallelic homologous recombination has been proposed as the underlying mechanism of CNVs in AZF regions. I...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000377649

    authors: Katsumi M,Ishikawa H,Tanaka Y,Saito K,Kobori Y,Okada H,Saito H,Nakabayashi K,Matsubara Y,Ogata T,Fukami M,Miyado M

    更新日期:2014-01-01 00:00:00

  • Interphase M-FISH applications using commercial probes in prenatal and PGD diagnostics.

    abstract::Early, rapid and reliable diagnosis is of first priority in prenatal medicine. The combination of specific sonographic markers (e.g. nuchal translucency) and biochemical parameters in maternal serum (e.g. free beta-human chorionic gonadotropin, pregnancy-associated plasma protein A), has already dramatically improved ...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000094217

    authors: Stumm M,Wegner RD,Bloechle M,Eckel H

    更新日期:2006-01-01 00:00:00

  • Meiotic mutations in rye Secale cereale L.

    abstract::Spontaneous meiotic mutations of winter rye Secale cereale L. (2n = 14) were revealed in inbred F2 progenies, which were obtained by self-pollination of F1 hybrids resulting from crosses of individual plants of cultivar Vyatka or weedy rye with plants of self-fertile inbred lines. The mutations cause partial or comple...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000082403

    authors: Sosnikhina SP,Mikhailova EI,Tikholiz OA,Priyatkina SN,Smirnov VG,Dadashev SY,Kolomiets OL,Bogdanov YF

    更新日期:2005-01-01 00:00:00

  • Elevated chromosome translocation frequencies in New Zealand nuclear test veterans.

    abstract::In 1957/58 the British Government conducted a series of nuclear tests in the mid-Pacific codenamed Operation Grapple, which involved several naval vessels from Britain and New Zealand. Two New Zealand frigates with 551 personnel onboard were stationed at various distances between 20 and 150 nautical miles from ground ...

    journal_title:Cytogenetic and genome research

    pub_type: 历史文章,杂志文章

    doi:10.1159/000125832

    authors: Wahab MA,Nickless EM,Najar-M'kacher R,Parmentier C,Podd JV,Rowland RE

    更新日期:2008-01-01 00:00:00

  • Cytogenetic and array-CGH characterization of a complex de novo rearrangement involving duplication and deletion of 9p and clinical findings in a 4-month-old female.

    abstract::Approximately 15 patients with partial trisomy 9p involving de novo duplications have been previously described. Here, we present clinical, cytogenetic, FISH and aCGH findings in a patient with a de novo complex rearrangement in the short arm of chromosome 9 involving an inverted duplication at 9p24-->p21.3 and a dele...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000251966

    authors: Hulick PJ,Noonan KM,Kulkarni S,Donovan DJ,Listewnik M,Ihm C,Stoler JM,Weremowicz S

    更新日期:2009-01-01 00:00:00

  • Genomic organization and expression pattern of scapinin (PHACTR3) in mouse and human.

    abstract::Scapinin has been found to bind to cytoplasmic actin and is also a putative regulatory subunit of protein phosphatase-1 (PP1). It is found attached to the nuclear matrix-intermediate filament (NM-IF) and is down-regulated by differentiation of tumor cells. We have analyzed the genomic structure and tissue-specific exp...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000094797

    authors: Worch S,Fiedler E,Hansmann I,Schlote D

    更新日期:2006-01-01 00:00:00

  • Cytogenetic mapping of 5S and 18S rRNAs and H3 histone genes in 4 ancient Proscopiidae grasshopper species: contribution to understanding the evolutionary dynamics of multigene families.

    abstract::This paper reports on the chromosomal location of 18S rRNA, 5S rRNA and H3 histone multigene families in 4 species of a relatively ancient and diversified group of grasshoppers belonging to the family Proscopiidae. The 5S rRNA and H3 histone genes were highly conserved in the number of sites and chromosomal position i...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000317476

    authors: Cabral-de-Mello DC,Martins C,Souza MJ,Moura RC

    更新日期:2011-01-01 00:00:00

  • Karyotypic evolution in the Galliformes: an examination of the process of karyotypic evolution by comparison of the molecular cytogenetic findings with the molecular phylogeny.

    abstract::To define the process of karyotypic evolution in the Galliformes on a molecular basis, we conducted genome-wide comparative chromosome painting for eight species, i.e. silver pheasant (Lophura nycthemera), Lady Amherst's pheasant (Chrysolophus amherstiae), ring-necked pheasant (Phasianus colchicus), turkey (Meleagris ...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000078570

    authors: Shibusawa M,Nishibori M,Nishida-Umehara C,Tsudzuki M,Masabanda J,Griffin DK,Matsuda Y

    更新日期:2004-01-01 00:00:00

  • Comparative Cytogenetics of the Congo African Grey Parrot (Psittacus erithacus).

    abstract::The Congo African grey parrot (Psittacus erithacus, PER) is an endemic species of Central Africa, valued for its intelligence and listed as vulnerable due to poaching and habitat destruction. Improved knowledge about the P. erithacus genome is needed to address key biological questions and conservation of this species...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000444136

    authors: Seibold-Torres C,Owens E,Chowdhary R,Ferguson-Smith MA,Tizard I,Raudsepp T

    更新日期:2015-01-01 00:00:00

  • Different numbers of rye B chromosomes induce identical compaction changes in distinct A chromosome domains.

    abstract::In rye each B chromosome (B) represents 5.5% of the diploid A genome. Rye Bs have several nuclear to whole plant effects although they seem to bear no genes except for the ones that lead to their maintenance within a population. In this context, and considering that rye Bs are enriched in repetitive non-coding regions...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000079306

    authors: Delgado M,Caperta A,Ribeiro T,Viegas W,Jones RN,Morais-Cecílio L

    更新日期:2004-01-01 00:00:00

  • Differential Dmrt1 transcripts in gonads of the protandrous black porgy, Acanthopagrus schlegeli.

    abstract::Black porgy, Acanthopagrus schlegeli Bleeker, is a marine protandrous hermaphrodite fish. A Dmrt1 cDNA was cloned and characterized and in order to study the process of sex change in this species, mRNA transcripts of Dmrt1 were monitored. Dmrt1 was specifically transcribed in testis and seminal vesicle in 2-year-old b...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000074354

    authors: He CL,Du JL,Wu GC,Lee YH,Sun LT,Chang CF

    更新日期:2003-01-01 00:00:00

  • New insights into the karyotypic relationships of Chinese muntjac (Muntiacus reevesi), forest musk deer (Moschus berezovskii) and gayal (Bos frontalis).

    abstract::To investigate the karyotypic relationships between Chinese muntjac (Muntiacus reevesi), forest musk deer (Moschus berezovskii) and gayal (Bos frontalis), a complete set of Chinese muntjac chromosome-specific painting probes has been assigned to G-banded chromosomes of these three species. Sixteen autosomal probes (i....

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000081520

    authors: Chi J,Fu B,Nie W,Wang J,Graphodatsky AS,Yang F

    更新日期:2005-01-01 00:00:00

  • The parasitic effects of rye B chromosomes might be beneficial in the long term.

    abstract::Rye B chromosomes (Bs) have strong parasitic effects on fertility. B carrying plants are less fertile than 0B ones, whereas the Bs have no significant effects on plant vigour. On the other hand, it has been reported that B transmission is under genetic control in such a way that H line plants transmit the Bs at high f...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000079316

    authors: González-Sánchez M,Chiavarino M,Jiménez G,Manzanero S,Rosato M,Puertas MJ

    更新日期:2004-01-01 00:00:00

  • Genotype-Phenotype Analysis, Neuropsychological Assessment, and Growth Hormone Response in a Patient with 18p Deletion Syndrome.

    abstract::18p deletion syndrome is a rare chromosomal disease caused by deletion of the short arm of chromosome 18. By using cytogenetic and SNP array analysis, we identified a girl with 18p deletion syndrome exhibiting craniofacial anomalies, intellectual disability, and short stature. G-banding analysis of metaphase cells rev...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000487371

    authors: Sun H,Wan N,Wang X,Chang L,Cheng D

    更新日期:2018-01-01 00:00:00

  • Comparative molecular cytogenetic studies in the order Carnivora: mapping chromosomal rearrangements onto the phylogenetic tree.

    abstract::We have made a set of chromosome-specific painting probes for the American mink by degenerate oligonucleotide primed-PCR (DOP-PCR) amplification of flow-sorted chromosomes. The painting probes were used to delimit homologous chromosomal segments among human, red fox, dog, cat and eight species of the family Mustelidae...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000063032

    authors: Graphodatsky AS,Yang F,Perelman PL,O'Brien PC,Serdukova NA,Milne BS,Biltueva LS,Fu B,Vorobieva NV,Kawada SI,Robinson TJ,Ferguson-Smith MA

    更新日期:2002-01-01 00:00:00

  • Polymorphisms of the chicken antiviral MX gene.

    abstract::The Mx gene was originally found in laboratory mice in an infection experiment using influenza virus (Lindermann, 1962). Almost all of the mouse strains in that experiment died from the infection, and only the A2G strain had resistance to the virus. This resistant character was shown to be inherited as a single autoso...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000103200

    authors: Watanabe T

    更新日期:2007-01-01 00:00:00

  • Cytogenetic Insights into the Evolution of Chromosomes and Sex Determination Reveal Striking Homology of Turtle Sex Chromosomes to Amphibian Autosomes.

    abstract::Turtle karyotypes are highly conserved compared to other vertebrates; yet, variation in diploid number (2n = 26-68) reflects profound genomic reorganization, which correlates with evolutionary turnovers in sex determination. We evaluate the published literature and newly collected comparative cytogenetic data (G- and ...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000447478

    authors: Montiel EE,Badenhorst D,Lee LS,Literman R,Trifonov V,Valenzuela N

    更新日期:2016-01-01 00:00:00

  • Epigenetic regulation of telomere maintenance.

    abstract::As chromatin structures, telomeres undergo epigenetic regulation of their maintenance and function. In plants, these processes are likely of a higher complexity than in animals or yeasts, as exemplified by methylation of cytosines in plant telomeric DNA or reversible developmental regulation of plant telomerase. We hi...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章,评审

    doi:10.1159/000360775

    authors: Fojtová M,Fajkus J

    更新日期:2014-01-01 00:00:00

  • Relating centromeric topography in fixed human chromosomes to α-satellite DNA and CENP-B distribution.

    abstract::Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates ...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000348744

    authors: Khan WA,Chisholm R,Tadayyon S,Subasinghe A,Norton P,Samarabandu J,Johnston LJ,Knoll JH,Rogan PK

    更新日期:2013-01-01 00:00:00

  • An easy protocol for studying chromatin and recombination protein dynamics during Arabidopsis thaliana meiosis: immunodetection of cohesins, histones and MLH1.

    abstract::Plant meiosis studies have enjoyed a fantastic boom in recent years with the use of Arabidopsis thaliana as a model not only for molecular genetics and genomics but also for cytogenetics. In this article we describe a new protocol for immunolabelling meiotic proteins that allows the detection of a large range of prote...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000314096

    authors: Chelysheva L,Grandont L,Vrielynck N,le Guin S,Mercier R,Grelon M

    更新日期:2010-07-01 00:00:00

  • 2q37.3 Deletion Syndrome: Two Cases with Highly Distinctive Facial Phenotype, Discordant Association with Schizophrenic Psychosis, and Shared Deletion Breakpoint Region on 2q37.3.

    abstract::2q37.3 deletion syndrome belongs to the chromosomal 2q37 deletion spectrum which clinically resembles Albright hereditary osteodystrophy (AHO) syndrome. It is is mainly characterized by short stature, obesity, round face, brachydactyly type E, intellectual disability, behavioral problems, and variable intellectual def...

    journal_title:Cytogenetic and genome research

    pub_type: 杂志文章

    doi:10.1159/000431389

    authors: Mehraein Y,Pfob M,Steinlein O,Aichinger E,Eggert M,Bubendorff V,Mannhart A,Müller S

    更新日期:2015-01-01 00:00:00