Abstract:
:Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of α-satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and α-satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Khan WA,Chisholm R,Tadayyon S,Subasinghe A,Norton P,Samarabandu J,Johnston LJ,Knoll JH,Rogan PKdoi
10.1159/000348744subject
Has Abstractpub_date
2013-01-01 00:00:00pages
234-42issue
4eissn
1424-8581issn
1424-859Xpii
000348744journal_volume
139pub_type
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
pub_type: 传,历史文章,杂志文章,评审
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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