Abstract:
:Holoprosencephaly (HPE) is the most common congenital malformation of the brain and face in humans. In this study we report the analysis of SIL (Sumacr;CL iumacr;nterrupting lumacr;ocus) as a candidate gene for HPE. Fluorescent in situ hybridization (FISH) analysis using a BAC 246e16 confirmed the assignment of SIL to 1p32. Computational analysis of SIL at the protein level revealed a 73% overall identity between the human and murine proteins. Denaturing high performance liquid chromatography (dHPLC) techniques were used to screen for mutations and these studies identified several common polymorphisms but no disease-associated mutations, suggesting that SIL is not a common factor in HPE pathogenesis in humans.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Karkera JD,Izraeli S,Roessler E,Dutra A,Kirsch I,Muenke Mdoi
10.1159/000064057keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
62-7issue
1-2eissn
1424-8581issn
1424-859Xpii
64057journal_volume
97pub_type
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