Abstract:
:In Europe, 2 different diploid chromosome numbers, 2n = 54 and 2n = 56, have been described in the lesser horseshoe bat (Rhinolophushipposideros). The eastern form with 2n = 56 extends from the Czech Republic to Greece. To date, specimens with 54 chromosomes have been reported only from Spain and Germany. This study expands the distributional area of the western variant to Ireland. Strikingly, this distribution of European chromosomal variants is in contrast to the available molecular data that indicate little genetic differentiation of R. hipposideros populations spanning Northwestern to Central Europe. Further, we have developed an optimized protocol for establishing fibroblast cell cultures, suitable for karyotype analyses, from 3-mm wing membrane biopsies. This is a useful technique for cytogenetic studies of endangered bat species, as this non-lethal sampling method imposes only minimum stress to the animal without lasting adverse effects and is routinely used to sample tissue probes for molecular genetic studies in bats.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Kacprzyk J,Teeling EC,Kelleher C,Volleth Mdoi
10.1159/000447111subject
Has Abstractpub_date
2016-01-01 00:00:00pages
279-83issue
4eissn
1424-8581issn
1424-859Xpii
000447111journal_volume
148pub_type
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