Abstract:
:Scapinin has been found to bind to cytoplasmic actin and is also a putative regulatory subunit of protein phosphatase-1 (PP1). It is found attached to the nuclear matrix-intermediate filament (NM-IF) and is down-regulated by differentiation of tumor cells. We have analyzed the genomic structure and tissue-specific expression pattern of both the human scapinin gene (PHACTR3) and the orthologous mouse gene. Both genes showed a highly conserved complex genomic organization with four different leader exons. Alternative splicing of exon 5 was found to be limited to human and variable polyadenylation in mouse transcripts only. In both species expression seems to occur predominantly in the brain. By Northern blot analysis two major transcripts in human and three transcripts in mouse were detected. Expression analysis in the mouse revealed a tissue-specific complex transcription pattern in the brain and a specific pattern was observed during prenatal development. Based on the transcriptional data we therefore assume scapinin to have a distinct biological function in the mammalian brain.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Worch S,Fiedler E,Hansmann I,Schlote Ddoi
10.1159/000094797subject
Has Abstractpub_date
2006-01-01 00:00:00pages
23-9issue
1eissn
1424-8581issn
1424-859Xpii
94797journal_volume
115pub_type
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