Abstract:
:We analyzed repeat sequences composition in the genome of cucumber inbred line 9930 using whole-genome shotgun reads. The analysis showed that satellite DNA sequences are the most dominant components in the cucumber genome. The distribution pattern of several tandem repeat sequences (Type I/II, Type III and Type IV) on cucumber chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of the Type III and 45S rDNA provide useful cytogenetic markers, whose position and fluorescence intensity allow for easy identification of all somatic metaphase chromosomes. A karyotype showing the position and fluorescence intensity of several tandem repeat sequences is constructed. The establishment of this FISH-based karyotype has created the basis for the integration of molecular, genetic and cytogenetic maps in Cucumis sativus and for the ultimate genome sequencing project as well.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Han YH,Zhang ZH,Liu JH,Lu JY,Huang SW,Jin WWdoi
10.1159/000151320subject
Has Abstractpub_date
2008-01-01 00:00:00pages
80-8issue
1eissn
1424-8581issn
1424-859Xpii
000151320journal_volume
122pub_type
杂志文章abstract::The ChickRH6 radiation hybrid panel has been used to construct consensus chromosome radiation hybrid (RH) maps of the chicken genome. Markers genotyped were either from throughout the genome or targeted to specific chromosomes and a large proportion (one third) of data was the result of collaborative efforts. Altogeth...
journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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abstract::Members of the NFAT (nuclear factors of activated T cells) gene family have been investigated in numerous organisms, including man and mouse. All NFATs may be synthesized in several isoforms differing in amino or carboxy termini due to 5' and 3' alternative splicing of the corresponding mRNA. Recently, we mapped the m...
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
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更新日期:2004-01-01 00:00:00
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journal_title:Cytogenetic and genome research
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doi:10.1159/000118745
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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pub_type: 杂志文章,评审
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