Abstract:
:Radiation-induced chromosome damage can be measured in interphase using the Premature Chromosome Condensation (PCC) technique. With the introduction of a new PCC technique using the potent phosphatase inhibitor calyculin-A, chromosomes can be condensed within five minutes, and it is now possible to examine the early damage induced by radiation. Using this method, it has been shown that high-LET radiation induces a higher frequency of chromatid breaks and a much higher frequency of isochromatid breaks than low-LET radiation. The kinetics of chromatid break rejoining consists of two exponential components representing a rapid and a slow time constant, which appears to be similar for low- and high-LET radiations. However, after high-LET radiation exposures, the rejoining process for isochromatid breaks influences the repair kinetics of chromatid-type breaks, and this plays an important role in the assessment of chromatid break rejoining in the G2 phase of the cell cycle.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Kawata T,Ito H,Uno T,Saito M,Yamamoto S,Furusawa Y,Durante M,George K,Wu H,Cucinotta FAdoi
10.1159/000077491keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
211-5issue
1-4eissn
1424-8581issn
1424-859Xpii
77491journal_volume
104pub_type
杂志文章,评审abstract::Approximately 15 patients with partial trisomy 9p involving de novo duplications have been previously described. Here, we present clinical, cytogenetic, FISH and aCGH findings in a patient with a de novo complex rearrangement in the short arm of chromosome 9 involving an inverted duplication at 9p24-->p21.3 and a dele...
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