Abstract:
:The detection of quantitative changes in genomic DNA, i.e. deletions and duplications or Copy Number Variants (CNVs), has recently gained considerable interest. First, detailed analysis of the human genome showed a surprising amount of CNVs, involving thousands of genes. Second, it was realised that the detection of CNVs as a cause of genetic disease was often neglected, but should be an essential part of a complete screening strategy. In both cases new efficient CNV screening methods, covering the entire range from specific loci to genome-wide, were behind these developments. This paper will briefly review the methods that are available to detect CNVs, discuss their strong and weak points, show some new developments and look ahead. Methods covered include microscopy, fluorescence in situ hybridization (including fiber-FISH), Southern blotting, PCR-based methods (including MLPA), array technology and massive parallel sequencing. In addition, we will show some new developments, including a 1400-plex CNV bead assay, fast-MLPA (from DNA to result in approximately 6 h) and a simple Melting Curve Analysis assay to confirm potential CNVs. Using the 1400-plex CNV bead assay, targeting selected chromosomal regions only, we detected confirmed rearrangements in 9% of 320 mental retardation patients studied.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Aten E,White SJ,Kalf ME,Vossen RH,Thygesen HH,Ruivenkamp CA,Kriek M,Breuning MH,den Dunnen JTdoi
10.1159/000184723subject
Has Abstractpub_date
2008-01-01 00:00:00pages
313-21issue
1-4eissn
1424-8581issn
1424-859Xpii
000184723journal_volume
123pub_type
杂志文章abstract::In an attempt to analyze the organization of repetitive DNAs in the amphibian genome, 7 microsatellite motifs and a 5S rDNA sequence were synthesized and mapped in the karyotypes of 5 Amolops species. The results revealed nonrandom distribution of the microsatellite repeats, usually in the heterochromatic regions, as ...
journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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更新日期:2006-01-01 00:00:00
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journal_title:Cytogenetic and genome research
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journal_title:Cytogenetic and genome research
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更新日期:2006-01-01 00:00:00
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journal_title:Cytogenetic and genome research
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