Abstract:
:Based on the analysis of 20 different monocot and eudicot species, we propose that the centromeric distribution of the phosphorylated histone H2AThr120 is evolutionary highly conserved across species with mono- and holocentric chromosomes. Therefore, antibodies recognizing the phosphorylated threonine 120 of the histone H2A can serve as a universal marker for the cytological detection of centromeres of mono- and holokinetic plant species. In addition, super resolution microscopy of signals specific to the centromere-specific histone H3 variant CENH3 and to H2AThr120ph revealed that these histone variants are incorporated into different nucleosomes, which form distinct, partly intermingled chromatin domains. This specific arrangement of both histone variants suggests different centromeric functions during the cell cycle.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Demidov D,Schubert V,Kumke K,Weiss O,Karimi-Ashtiyani R,Buttlar J,Heckmann S,Wanner G,Dong Q,Han F,Houben Adoi
10.1159/000360018subject
Has Abstractpub_date
2014-01-01 00:00:00pages
150-6issue
1-3eissn
1424-8581issn
1424-859Xpii
000360018journal_volume
143pub_type
杂志文章abstract::B chromosomes are now known in eight Brazilian rodent species: Akodon montensis, Holochilus brasiliensis, Nectomys rattus, N. squamipes, Oligoryzomys flavescens, Oryzomys angouya, Proechimys sp. 2 and Trinomys iheringi. Typically these chromosomes are heterogeneous relative to size, morphology, banding patterns, prese...
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