Abstract:
:Tissue-engineered skin substitutes provided a feasibility to overcome the shortage of skin autograft by culturing keratinocytes and dermal fibroblasts in vitro. In this study, we applied bi-layer gelatin-chondrointin-6-sulfate-hyaluronic acid (gelatin-C6S-HA) biomatrices onto the severe combined immunodeficiency (SCID) mice to evaluate its effect on promoting wound healing. Human foreskin keratinocytes and dermal fibroblasts were cultured with reconstructed skin equivalent (rSE) for 7 days. The rSE was then grafted to the dorsum of SCID mice to evaluate its biocompatibility by histologic and immunohistochemistry analysis. The results showed that human epidermis were well-developed with the expression of differentiated markers and basement membrane-specific proteins at 4 weeks. After implantation, the percentages of skin graft take were satisfactory, while cell-seeded group was better than non-cell-seeded one. The basement membrane proteins including laminin, type IV collagen, type VII collagen, integrin alpha6, and integrin beta4 were all detected at the dermal-epidermal junction, which showed a continuous structure in the 4 weeks after grafting. This bi-layer gelatin-C6S-HA skin substitute not only has positive effect on promoting wound healing, but also has high rate of graft take. This rSE would have the potential to be applied on the extensively and deeply burned patients who suffer from severe skin defect in the near future.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Wang TW,Sun JS,Wu HC,Tsuang YH,Wang WH,Lin FHdoi
10.1016/j.biomaterials.2006.07.024subject
Has Abstractpub_date
2006-11-01 00:00:00pages
5689-97issue
33eissn
0142-9612issn
1878-5905pii
S0142-9612(06)00641-7journal_volume
27pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::We investigated the hypothesis that immobilizing TGF-β1 within fibrin hydrogels may act in synergy with cyclic mechanical stimulation to enhance the properties of vascular grafts. To this end, we engineered a fusion TGF-β1 protein that can covalently anchor to fibrin during polymerization upon the action of factor XII...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.05.073
更新日期:2013-10-01 00:00:00
abstract::Understanding mechanisms that govern cell fate determination of human induced pluripotent stem cells (hiPSCs) could assist in maintenance of the undifferentiated state during cell expansion. We used polyamidoamine dendrimer surfaces with first-generation (G1), third-generation (G3) and fifth-generation (G5) of dendron...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.03.085
更新日期:2014-07-01 00:00:00
abstract::D-α-tocopheryl polyethylene glycol succinate (Vitamin E TPGS, or simply TPGS) is a water-soluble derivative of natural Vitamin E, which is formed by esterification of Vitamin E succinate with polyethylene glycol (PEG). As such, it has advantages of PEG and Vitamin E in application of various nanocarriers for drug deli...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2012.03.046
更新日期:2012-06-01 00:00:00
abstract::Tissue engineering requires the use of polymeric scaffolds that mimic many roles of extracellular matrices (ECM) in the body. Controlling cell-scaffold interactions is one of the most critical parameters for regulating cell phenotype in tissue engineering, and a peptide with the sequence of RGD has been widely exploit...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.03.063
更新日期:2010-07-01 00:00:00
abstract::Bioactive glass granules were implanted in the tibiae of rabbits in order to determine the pathway of the silicon released from bioactive glass in vivo. We traced and quantified the silicon released by obtaining 24-h urine samples and blood samples for up to 7 months after implantation. Bone tissue as well as the foll...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00097-7
更新日期:2002-01-01 00:00:00
abstract::Severe wear of the ultra-high molecular weight polyethylene (UHMWPE) components of total joint replacements limits their long-term success. In previous studies, the infrared spectra obtained on retrieved UHMWPE components were interpreted as evidence that the UHMWPE oxidizes in vivo. In direct contrast, infrared spect...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(93)90062-7
更新日期:1993-07-01 00:00:00
abstract::We developed a strategy to formulate supraparamagnetic iron oxides (SPIOs) in nanoparticles (NPs) of biodegradable copolymer made up of poly(lactic acid) (PLA) and d-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS) for medical imaging by magnetic resonance imaging (MRI) of high contrast and low side effects....
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.03.070
更新日期:2010-07-01 00:00:00
abstract::The surface and bulk properties of novel methacrylate polymers prepared by gelling poly(ethyl methacrylate) (PEMA) powder with different ratios of tetrahydrofurfuryl methacrylate (THFMA) and hydroxyethyl methacrylate (HEMA) monomers were investigated. The water adsorption and desorption characteristics of these polyme...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00229-5
更新日期:2001-04-01 00:00:00
abstract::The main challenge for neural progenitor cell (NPC)-mediated repair of spinal cord injury (SCI) is lack of favorable environment to direct its differentiation towards neurons rather than glial cells. The myelin associated inhibitors have been demonstrated to promote NPC differentiation into glial lineage. Herein, to i...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.03.062
更新日期:2013-07-01 00:00:00
abstract::Surface microroughness increases osteoblast differentiation and enhances responses of osteoblasts to 1,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3]. The observations that beta1 integrin expression is increased in osteoblasts grown on Ti substrates with rough microarchitecture, and that it is regulated by 1alpha,25(OH)2D3...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.02.022
更新日期:2006-07-01 00:00:00
abstract::We design a class of water-dispersible hybrid nanogels for intracellular delivery of hydrophobic curcumin. The core-shell structured hybrid nanogels were synthesized by coating the Ag/Au bimetallic nanoparticles (NPs) with a hydrophobic polystyrene (PS) gel layer as inner shell, and a subsequent thin hydrophilic nonli...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.08.112
更新日期:2011-01-01 00:00:00
abstract::Wear particle-induced periprosthetic osteolysis has been a clinical problem driving the development of wear resistant ultrahigh molecular weight polyethylene (UHMWPE) for total joint replacement. Radiation crosslinking has been used to decrease wear through decreased plastic deformation; but crosslinking also reduces ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.05.041
更新日期:2010-09-01 00:00:00
abstract::This study attempted to enhance the efficacy of peripheral nerve regeneration using our previously tested poly(L-lactic acid) (PLLA) conduits by incorporating them with allogeneic Schwann cells (SCs). The SCs were harvested, cultured to obtain confluent monolayers and two concentrations (1 x 10(4) and 1 x 10(6) SC/ml)...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00190-9
更新日期:2002-02-01 00:00:00
abstract::The flexural strength of five glass ionomers was measured at intervals ranging from 1 h to 3 months after mixing. The pattern of change in flexural strength is material-specific with two water-activated materials having significantly higher long-term flexural strengths than the others. This may be attributable to the ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90113-o
更新日期:1991-09-01 00:00:00
abstract::Thrombomodulin (TM) is a newly described endothelial cell-associated protein that functions as a potent natural anticoagulant by converting thrombin from a procoagulant protease to an anticoagulant. In this study, focussing on the application of TM for biomedical materials, recombinant human TM (hTM) was immobilized o...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(94)90041-8
更新日期:1994-08-01 00:00:00
abstract::To fully understand biological behavior in vitro often dictates that oxygen be reported at either a local or a cellular level. Oxygen sensors based on the luminescent quenching of a specific form of electrospun fiber were developed for measurement of both gaseous and dissolved oxygen concentrations. Electrospinning wa...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.055
更新日期:2016-01-01 00:00:00
abstract::The in vivo performance of 250 retrieved internal fixation plates was evaluated. The corrosion characteristics and metallurgical properties of each implant were assessed and correlated with respective clinical performance. Screw-plate interface corrosion and screw surface corrosion were graded; Rockwell hardness, grai...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(87)90060-3
更新日期:1987-05-01 00:00:00
abstract::We show here, that the proapoptotic peptide, KLAKLAKKLAKLAK (KLA), which by itself does not penetrate cell membranes, can do so when fused to a protein transduction domain derived from NH(2)-terminus of translationally controlled tumor protein (TCTP-PTD, MIIYRDLISH). Once inside the cell, the conjugated KLA exerts its...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.03.074
更新日期:2011-08-01 00:00:00
abstract::Metal sensitivity (MS) reactions to implant metals represent a rare but well-documented complication following total joint arthroplasty (TJA). Although 20-25% of post-TJA patients develop MS, only a few highly susceptible patients (< 1%) exhibit symptoms. Whether surgeons should perform screening for MS is currently a...
journal_title:Biomaterials
pub_type: 临床试验,杂志文章
doi:10.1016/j.biomaterials.2004.03.038
更新日期:2005-03-01 00:00:00
abstract::This article introduces novel biocompatible tissue adhesives that do not involve any chemical or biochemical reactions, during their application in vivo. The use of these new adhesives is based exclusively on their temperature-dependent rheological properties. Since biocompatibility and biodegradability are additional...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.01.040
更新日期:2004-12-01 00:00:00
abstract::Compression, tension and torsion tests were designed and completed successfully on a brushite and a precipitated hydroxyapatite cement in moist condition. Elastic and strength properties were measured for these three loading cases. For each cement, the full set of strength data was fitted to an isotropic Tsai-Wu crite...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00041-2
更新日期:2001-11-01 00:00:00
abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.04.046
更新日期:2008-06-03 00:00:00
abstract::We present a dual-drug delivery system (DDDS) of hydrogel/polypeptide micelle composites in this work. The DDDS was constructed from aspirin (Asp) dispersed poly(vinyl alcohol) (PVA) or Chitosan (CS)/PVA hydrogel and doxorubicin (DOX) loaded poly(L-glutamic acid)-b-poly(propylene oxide)-b-poly(L-glutamic acid) (GPG) m...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.01.006
更新日期:2009-05-01 00:00:00
abstract::microRNA-124 (miR-124), the most abundant miRNA of the CNS, was recently shown to modulate the polarization of activated microglia and infiltrating macrophages towards the anti-inflammatory M2 phenotype and protect neurons in various ways after brain disease. In ischemic stroke, microglia and macrophages of a detrimen...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.03.025
更新日期:2016-06-01 00:00:00
abstract::In this study, a type of intracellular redox-triggered hollow mesoporous silica nanoreservoirs (HMSNs) with tumor specificity was developed in order to deliver anticancer drug (i.e., doxorubicin (DOX)) to the target tumor cells with high therapeutic efficiency and reduced side effects. Firstly, adamantanamine was graf...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.05.058
更新日期:2014-09-01 00:00:00
abstract::Demineralised bone matrix (DBM) is a form of allogeneic tissue graft widely used in oral and maxillofacial procedures. There is a long history of controversy relating to the suitability of ethylene oxide gas (EtOx) as a terminal sterilisation agent for this graft, relating to its effects on the clinical performance of...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00255-6
更新日期:2001-05-01 00:00:00
abstract::Stem cell therapy is a promising therapeutic method for the treatment of ischemic heart diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac re...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.10.026
更新日期:2017-01-01 00:00:00
abstract::This study is concerned with the effect of dual implantation of calcium and phosphorus upon the structure, corrosion resistance and biocompatibility of titanium. The ions were implanted in sequence, first Ca and then P, both at a dose of 10(17) ions/cm2 at a beam energy of 25 keV. Transmission electron microscopy was ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.08.015
更新日期:2005-06-01 00:00:00
abstract::Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transf...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.05.070
更新日期:2011-10-01 00:00:00
abstract::Semiconductor quantum dots and upconversion nanoparticles have been broadly used for live cell imaging due to their color tunability and photostability etc. However, these inorganic materials often contain heavy metals and potentially have metabolism problems. To overcome these issues, herein, we report a type of orga...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.03.045
更新日期:2016-07-01 00:00:00