Abstract：
:The main challenge for neural progenitor cell (NPC)-mediated repair of spinal cord injury (SCI) is lack of favorable environment to direct its differentiation towards neurons rather than glial cells. The myelin associated inhibitors have been demonstrated to promote NPC differentiation into glial lineage. Herein, to inhibit the downstream signaling activated by myelin associated inhibitors, cetuximab, an epidermal growth factor receptor (EGFR) neutralizing antibody, functionalized collagen scaffold has been developed as a vehicle for NPC implantation. It was found that collagen-cetuximab 1 μg scaffolds enhanced neuronal differentiation and inhibited astrocytic differentiation of NPCs exposed to myelin proteins significantly in vitro. To test the therapeutic effect in vivo, NPCs expressing green fluorescent protein (GFP)-embedded scaffolds have been implanted into the 4 mm-long hemisection lesion of rats. We found that the collagen-cetuximab 5 μg scaffolds induced neuronal differentiation and decreased astrocytic differentiation of NPCs, enhanced axon regeneration, and promoted functional recovery markedly. A well-functionalized scaffold was constructed to improve the recovery of SCI, which could promote the neuronal differentiation of neural progenitor cells in vivo.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Li X,Xiao Z,Han J,Chen L,Xiao H,Ma F,Hou X,Li X,Sun J,Ding W,Zhao Y,Chen B,Dai Jdoi
10.1016/j.biomaterials.2013.03.062subject
Has Abstractpub_date
2013-07-01 00:00:00pages
5107-16issue
21eissn
0142-9612issn
1878-5905pii
S0142-9612(13)00384-0journal_volume
34pub_type
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