Abstract:
:A PCL/hydrogel construct that would mimic the structural organization, biochemistry and anatomy of meniscus was engineered. The compressive (380 ± 40 kPa) and tensile modulus (18.2 ± 0.9 MPa) of the PCL scaffolds were increased significantly when constructs were printed with a shifted design and circumferential strands mimicking the collagen organization in native tissue (p < 0.05). Presence of circumferentially aligned PCL strands also led to elongation and alignment of the human fibrochondrocytes. Gene expression of the cells in agarose (Ag), gelatin methacrylate (GelMA), and GelMA-Ag hydrogels was significantly higher than that of cells on the PCL scaffolds after a 21-day culture. GelMA exhibited the highest level of collagen type I (COL1A2) mRNA expression, while GelMA-Ag exhibited the highest level of aggrecan (AGG) expression (p < 0.001, compared to PCL). GelMA and GelMA-Ag exhibited a high level of collagen type II (COL2A1) expression (p < 0.05, compared to PCL). Anatomical scaffolds with circumferential PCL strands were impregnated with cell-loaded GelMA in the periphery and GelMA-Ag in the inner region. GelMA and GelMA-Ag hydrogels enhanced the production of COL 1 and COL 2 proteins after a 6-week culture (p < 0.05). COL 1 expression increased gradually towards the outer periphery, while COL 2 expression decreased. We were thus able to engineer an anatomical meniscus with a cartilage-like inner region and fibrocartilage-like outer region.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Bahcecioglu G,Bilgen B,Hasirci N,Hasirci Vdoi
10.1016/j.biomaterials.2019.119361subject
Has Abstractpub_date
2019-10-01 00:00:00pages
119361eissn
0142-9612issn
1878-5905pii
S0142-9612(19)30460-0journal_volume
218pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::A biocompatible hydrogel of poly[N-(2-hydroxypropyl)methacrylamide] (PHPMA) which includes the cell-adhesive region of fibronectin Arg-Gly-Asp was synthesized and its structure, rheological and dielectric properties were characterized. The ability of a PHPMA-RGD hydrogel to promote tissue regeneration and support axon...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00354-9
更新日期:2001-05-01 00:00:00
abstract::The purpose of this study was to investigate the chemical influence of chemo-mechanical caries removal (CMCR) on surface topography of dental ceramic materials. Thirty samples of three different types of ceramics (Vita Omega, Vita Alpha, and Procera AllTitan) were manufactured and used. With an optical profilometer, d...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00141-2
更新日期:2002-10-01 00:00:00
abstract::Quinacrine and related heterocyclic compounds have antiprion activity. Since the infectious pathogen of prion diseases is composed of multimeric PrP(Sc) assemblies, we hypothesized that this antiprion property could be enhanced by attaching multiple quinacrine-derived chloroquinoline or acridine moieties to a scaffold...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.06.004
更新日期:2012-10-01 00:00:00
abstract::The objective of this study was to investigate the effect of repeated applications of a neutral NaF solution on the surface roughness of four conventional glass ionomer cements (GIC) (ChemFil Superior encapsulated, Fuji Cap II, Ketac-Fil and Hi Dense), three resin-modified (RM-) GIC (Fuji II LC encapsulated, Photac-Fi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00617-8
更新日期:2003-05-01 00:00:00
abstract::Polyvinyl alcohol (PVA) cylindrical hydrogel with a stiffness gradient was prepared using a simple liquid nitrogen (LN2)-contacting gradual freezing and thawing method in order to investigate the effects of substrate stiffness on stem cell differentiation into specific cell types. The prepared cylindrical PVA hydrogel...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.11.017
更新日期:2015-02-01 00:00:00
abstract::Semiconduction activation energies of adenine, guanine, thymine and uracil complexes with acridine orange dye at various dye concentrations were measured in both Ohmic and space charge limited (SCL) current regimes. The behaviour of the complexes is non-extrinsic at high dye concentrations but extrinsic at low dye con...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(89)90025-2
更新日期:1989-04-01 00:00:00
abstract::Recapitulation of the cellular microenvironment of the heart, which promotes cell contraction, remains a key challenge in cardiac tissue engineering. We report here on our work, where for the first time, a 3-dimensional (3D) spring-like fiber scaffold was fabricated, successfully mimicking the coiled perimysial fibers...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.07.054
更新日期:2013-11-01 00:00:00
abstract::Islet-like cells derived from embryonic stem (ES) cells may be a promising therapeutic option for future diabetes treatment. Here, we demonstrated a five-stage protocol with adding exendin-4 instead of nicotinamide finally could generate islet-like cells from human embryonic stem (ES) cells. Immunofluorescence analysi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.12.030
更新日期:2009-03-01 00:00:00
abstract::The in vitro labelling of cultured cells with iron oxide nanoparticles (NPs) is a frequent practice in biomedical research. To date, the potential cytotoxicity of these particles remains an issue of debate. In the present study, 4 different NP types (dextran-coated Endorem, carboxydextran-coated Resovist, lipid-coated...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.08.075
更新日期:2011-01-01 00:00:00
abstract::Hydroxy-fluorapatites (OH-FAps) occur biologically in teeth and form the basis for application as biomaterials. This work aims to synthesize a series of fluoride substituted calcium hydroxyapatites (OHAps) to determine how fluoride influences the synthesis and the resulting characteristics of solid solutions. OH-FAPs ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00259-x
更新日期:2003-09-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) hold great promise as a cell source for regenerative medicine yet its culture, maintenance of pluripotency and induction of differentiation remain challenging. Conversely, graphene (G) and graphene oxide (GO) have captured tremendous interests in the fields of materials science, ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.09.071
更新日期:2012-01-01 00:00:00
abstract::The oriented attachment of hydroxyapatite (HAP) nanorods was achieved through a hydrothermal treatment. The nanorods were arranged in order along the c-axis of the HAP. These formed ca. 3.5 slip-shaped pores between the rods. High-resolution transmission electron (HRTEM) also revealed that single crystals of HAP may g...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.01.033
更新日期:2007-05-01 00:00:00
abstract::Alzheimer's disease (AD) is characterized by the loss of neurogenesis and excessive induction of apoptosis. The induction of neurogenesis and inhibition of apoptosis may be a promising therapeutic approach to combating the disease. Celecoxib (CB), a cyclooxygenase-2 specific inhibitor, could offer neuroprotection. Spe...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.07.023
更新日期:2017-11-01 00:00:00
abstract::In the present study we compare LPEI and BPEI characteristics related to DNA condensation and their role as free polycation chains in gene transfection. Using radioactive (32)P labeled DNA, we investigated the effect of free PEI chains on the cellular uptake of polyplexes. Our investigations show different properties ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.07.044
更新日期:2011-11-01 00:00:00
abstract::Tarsal repair is an important part for eyelid reconstruction. Presently traditional clinic treatments do not produce satisfactory repair effects. The key is to find a proper tarsal repair material. Microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) was studied for application as tarsal substitute in this...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.06.044
更新日期:2010-10-01 00:00:00
abstract::The release of the anti-inflammatory agent dexamethasone (DEX) from nanoparticles of poly(lactic-co-glycolic acid) (PLGA) embedded in alginate hydrogel (HG) matrices was investigated. DEX-loaded PLGA nanoparticles were prepared using a solvent evaporation technique and were characterized for size, drug loading, and in...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.12.021
更新日期:2006-05-01 00:00:00
abstract::The sintering behavior of calcium pyrophosphate (CPP, Ca2P2O7)-doped beta-tricalcium phosphate [TCP, Ca3(PO4)2], prepared by solid state reaction, was investigated in-situ, using dilatometry. Pure beta-TCP undergoes phase transition to alpha-TCP at about 1200 degrees C; hence pure beta-TCP ceramics should be sintered ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00201-0
更新日期:2002-02-01 00:00:00
abstract::The objective of this research was to investigate whether immobilized anti-inflammatory cytokines will signal changes in the inflammatory profile of cultured monocytes. A fusion protein of recombinant human IL-1 receptor antagonist and elastin-like peptide (IL-1ra-ELP) was expressed in Escherichia coli. THP-1 human mo...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.04.010
更新日期:2007-08-01 00:00:00
abstract::A amphiphilic block copolymer composed of conventional monomethoxy (polyethylene glycol)-poly (d,l-lactide-co-glycolide)-poly (l-lysine) (mPEG-PLGA-b-PLL) was synthesized. The chemical structure of this copolymer and its precursors was confirmed by Fourier Transform Infrared Spectroscopy (FTIR), (1)H Nuclear Magnetic ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.02.041
更新日期:2012-06-01 00:00:00
abstract::Pancreatic cancer is predicted to be the second leading cause of cancer-related death by 2025. The best chemotherapy only extends survival by an average of 18 weeks. The extensive fibrotic stroma surrounding the tumor curbs therapeutic options as chemotherapy drugs cannot freely penetrate the tumor. RNA interference (...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2019.119742
更新日期:2020-05-01 00:00:00
abstract::Hyaluronic acid (HA) hydrogels have found a wide range of applications in biomedicine: regenerative medicine to drug delivery applications. In vivo quantitative assessment of these hydrogels using magnetic resonance imaging (MRI) provides an effective, accurate, safe, and non-invasive translational approach to assess ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2018.05.037
更新日期:2018-09-01 00:00:00
abstract::In the present study, the effects of implant design and surface properties on peri-implant bone response were evaluated with both conventional histomorphometry and micro-computed tomography (micro-CT), using two geometrically different dental implants (Screw type, St; Push-in, Pi) either or not surface-modified (non-c...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.05.017
更新日期:2009-09-01 00:00:00
abstract::Polypyrrole (PPy) is a conducting polymer and is obtained electrochemically on an electrode such as indium-tin oxide (ITO). In this study, in order to develop a novel cell-culture system which makes it possible to communicate with cultured mammalian cells, bovine adrenal chromaffin cells were cultured on PPy-coated IT...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(96)00015-4
更新日期:1996-10-01 00:00:00
abstract::The flexural strength of five glass ionomers was measured at intervals ranging from 1 h to 3 months after mixing. The pattern of change in flexural strength is material-specific with two water-activated materials having significantly higher long-term flexural strengths than the others. This may be attributable to the ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90113-o
更新日期:1991-09-01 00:00:00
abstract::Cancer therapeutics are developed through extensive screening; however, many therapeutics evaluated with 2D in vitro cultures during pre-clinical trials suffer from lower efficacy in patients. Replicating the in vivo tumor microenvironment in vitro with three-dimensional (3D) porous scaffolds offers the possibility of...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.09.034
更新日期:2013-12-01 00:00:00
abstract::Poly-γ-glutamic acid (γ-PGA) is an anionic polypeptide secreted by Bacillus sp. that has been shown to activate immune cells through interactions with toll-like receptor 4 (TLR4). However, its ability to induce the type I interferon (IFN) response has not yet been characterized. Here, we demonstrate that γ-PGA induces...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.08.067
更新日期:2013-12-01 00:00:00
abstract::Temperature-responsive polymers are attractive candidates for applications related to injectable delivery of biologically active therapeutics, such as stem cells. In this study, we evaluate the potential of thermosensitive hydroxybutyl chitosan (HBC) as a biomaterial for the culture of human mesenchymal stem cells (hM...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.07.033
更新日期:2006-01-01 00:00:00
abstract::Bioactive glass granules were implanted in the tibiae of rabbits in order to determine the pathway of the silicon released from bioactive glass in vivo. We traced and quantified the silicon released by obtaining 24-h urine samples and blood samples for up to 7 months after implantation. Bone tissue as well as the foll...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00097-7
更新日期:2002-01-01 00:00:00
abstract::Cutaneous melanoma is an increasingly common and potentially lethal malignancy of melanocytes, the melanin producing cells normally located in the basal layer of the skin epidermis. Despite major advances in cancer chemotherapeutics and immunotherapy, the success in treating metastatic melanoma remains poor. The notio...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.08.056
更新日期:2011-12-01 00:00:00
abstract::Lymph nodes (LNs) are immune organs housing high concentrations of lymphocytes, making them critical targets for therapeutic immunomodulation in a wide variety of diseases. While there is great interest in targeted drug delivery to LNs, many nanoscale drug delivery carriers have limited access to parenchymal resident ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120411
更新日期:2021-01-01 00:00:00