Abstract：
:In the present study we compare LPEI and BPEI characteristics related to DNA condensation and their role as free polycation chains in gene transfection. Using radioactive (32)P labeled DNA, we investigated the effect of free PEI chains on the cellular uptake of polyplexes. Our investigations show different properties of BPEI and LPEI polyplexes in condensation and de-condensation processes as well as in cellular uptake, which was tightly correlated with transfection efficiency. In agreement with earlier reports we find all DNA to be condensed at N/P = 3. Further added PEI chains remain free in solution. We found that both the cellular uptake and gene transfection of BPEI polyplexes is much more efficient than LPEI polyplexes at a low N/P ratio of 3 (i.e., without free PEI chains). When N/P is high (10, with 7 portions of free PEI), the LPEI and BPEI polyplexes have similar transfection efficiency even though the cellular uptake of the LPEI polyplexes is significantly lower. In addition, we found that addition of free short or long PEI chains (2.5 and 25 kDa) leads to a comparable gene transfection efficiency.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Dai Z,Gjetting T,Mattebjerg MA,Wu C,Andresen TLdoi
10.1016/j.biomaterials.2011.07.044subject
Has Abstractpub_date
2011-11-01 00:00:00pages
8626-34issue
33eissn
0142-9612issn
1878-5905pii
S0142-9612(11)00827-1journal_volume
32pub_type
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