Abstract:
:Primary canine oral mucosal epithelial cells were cultured on temperature-responsive dishes and cell culture inserts to fabricate transplantable epithelial cell sheets. When 3T3 feeder layers and fetal bovine serum were eliminated from dish culture, the harvested cell sheets became significantly more fragile. In contrast, when epithelial cells were cultured on inserts having submicron-scale pores, cell sheet fragility was eliminated. Keratin expression profiles showed no differences among the harvested cell sheets, but the expression of p63, a putative stem/progenitor marker, was strongly dependent on the presence of 3T3 feeder layers and serum. These results suggest that the maintenance of stem/progenitor cells is influenced by the apical/basal supply of nutrients as well as culture supplements.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Murakami D,Yamato M,Nishida K,Ohki T,Takagi R,Yang J,Namiki H,Okano Tdoi
10.1016/j.biomaterials.2006.05.042subject
Has Abstractpub_date
2006-11-01 00:00:00pages
5518-23issue
32eissn
0142-9612issn
1878-5905pii
S0142-9612(06)00470-4journal_volume
27pub_type
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