Abstract:
:We show here, that the proapoptotic peptide, KLAKLAKKLAKLAK (KLA), which by itself does not penetrate cell membranes, can do so when fused to a protein transduction domain derived from NH(2)-terminus of translationally controlled tumor protein (TCTP-PTD, MIIYRDLISH). Once inside the cell, the conjugated KLA exerts its proapoptotic activity to inhibit tumor growth. We evaluated the cellular uptake of KLA fused to TCTP-PTD (hereafter called TCTP-KLA) and its effect on cancer cell viability. The IC(50) of TCTP-KLA was between 7 and 10 μmol/L. We also evaluated its anti-tumor activity in vivo by injecting it into xenografts of lung carcinoma in Balb/c nude mice. Tumor growth inhibition resulting from treatment with TCTP-KLA was better than that of TAT-KLA. These results suggest that TCTP-KLA can be applied to design cancer therapeutics.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Kim HY,Kim S,Youn H,Chung JK,Shin DH,Lee Kdoi
10.1016/j.biomaterials.2011.03.074subject
Has Abstractpub_date
2011-08-01 00:00:00pages
5262-8issue
22eissn
0142-9612issn
1878-5905pii
S0142-9612(11)00372-3journal_volume
32pub_type
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