Immunoreactivity of boronated antibodies.

abstract：:CD3-specific antibodies have shown clinical efficacy in both transplantation and autoimmunity. However, targeting CD3 in this way can lead to T-cell activation and a serious cytokine release syndrome mediated by Fcγ receptor binding. An in vivo mouse model has been developed using severe combined immunodeficient (SCID...

journal_title：Journal of immunological methods

authors： Malcolm SL,Smith EL,Bourne T,Shaw S

更新日期：2012-10-31 00:00:00

abstract：:Upon treatment with protein therapeutics, a subset of patients will typically develop antibodies against the drug. These anti-drug antibodies can be of concern because they have the potential to alter the drug's therapeutic activity. In the case of relapsing-remitting multiple sclerosis (RRMS) patients receiving recom...

journal_title：Journal of immunological methods

authors： Brickelmaier M,Hochman PS,Baciu R,Chao B,Cuervo JH,Whitty A

更新日期：1999-07-30 00:00:00

abstract：:The length distributions of the third complementarity determining region (CDR3) of the T cell receptor (TCR) beta chain were determined quantitatively from peripheral blood lymphocytes of healthy donors. RT-PCR products of 26 V beta families and subfamilies were analyzed by an A.L.F. DNA sequencer and Fragment Manager...

journal_title：Journal of immunological methods

authors： Liu D,Callahan JP,Dau PC

更新日期：1995-11-16 00:00:00

abstract：:An enzyme linked immunosorbent assay (ELISA) method for the detection of antibodies to platelets and leucocytes is presented. The method can be used for large numbers of samples. The method is objective when photometers are used. Approximately 50% of all cases of possible autoimmune thrombocytopenic purpura (A.T.P.) a...

journal_title：Journal of immunological methods

authors： Doughty R,James V,Magee J

更新日期：1981-01-01 00:00:00

abstract：:Non-specific radioactive counts retained on the glass fiber filters from a multiple automatic sample harvester were reduced markedly by 3 simple changes in the procedure for harvesting thymidine-labeled whole blood cultured from microtiter plates. These modifications increased the sensitivity, precision and accuracy o...

journal_title：Journal of immunological methods

authors： Kaplan JH,Razzano AF,Bergeron JA

更新日期：1981-01-01 00:00:00

abstract：:The antigen capture capacity of antibodies covalently immobilized on injection-molded polystyrene beads was evaluated. Bromoacetyl groups on the bead surfaces rendered them reactive to protein nucleophilic groups. The bromoacetyl surface exhibited up to a ten-fold greater capacity for protein compared to unmodified po...

journal_title：Journal of immunological methods

authors： Peterman JH,Tarcha PJ,Chu VP,Butler JE

更新日期：1988-07-22 00:00:00

abstract：:A separation procedure based on Percoll gradient centrifugation is described here that allows enrichment for two main populations of granules, with average densities of 1.05 and 1.1 g/ml. The heavy-density granules, which comprise 5-20% of the total amount of membrane-lytic activity, are morphologically and biochemica...

journal_title：Journal of immunological methods

authors： Persechini PM,Liu CC,Detmers PA,Young JD

更新日期：1989-11-13 00:00:00

abstract：:Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial ...

journal_title：Journal of immunological methods

authors： De Plano LM,Fazio E,Rizzo MG,Franco D,Carnazza S,Trusso S,Neri F,Guglielmino SPP

更新日期：2019-02-01 00:00:00

abstract：:A method is described for the isolation of filamentous phage-displayed human monoclonal antibodies directed at unpurifiable cell surface-expressed molecules. To optimize the capture of antigen-specific phage and minimize the binding of irrelevant phage antibodies, a simultaneous positive and negative selection strateg...

journal_title：Journal of immunological methods

authors： Siegel DL,Chang TY,Russell SL,Bunya VY

更新日期：1997-08-07 00:00:00

abstract：:CFSE analysis of a proliferating cell population is a popular tool for the study of cell division and divisionlinked changes in cell behavior. Recently Banks et al. (2011), Luzyanina et al. (2009), Luzyanina et al. (2007), a partial differential equation (PDE) model to describe lymphocyte dynamics in a CFSE proliferat...

journal_title：Journal of immunological methods

authors： Banks HT,Sutton KL,Thompson WC,Bocharov G,Doumic M,Schenkel T,Argilaguet J,Giest S,Peligero C,Meyerhans A

更新日期：2011-10-28 00:00:00

abstract：:Historically, tumour markers are substances selectively released by tumour cells in the blood stream so that they can be detected in the serum or other body fluids for clinical monitoring of various malignancies. The term is now extended to cell or tissue characteristics, such as cytogenetic markers, oncogenes or abno...

journal_title：Journal of immunological methods

authors： Magdelénat H

更新日期：1992-06-24 00:00:00

abstract：:A standardized, reproducible two-step method for separation of human peripheral blood monocytes on continuous Percoll gradients has been developed. The first step involves separation of mononuclear cell on Percoll of density 1.075 g/ml and the second step separation of monocytes from lymphocytes on a continuous Percol...

journal_title：Journal of immunological methods

authors： Brandslund I,Rasmussen JM,Fisker D,Svehag SE

更新日期：1982-01-01 00:00:00

abstract：:In order to isolate monoclonal antibodies (mAbs) that bind to tumor-associated antigens (Ags) we developed the following strategy. Using the phage-display Ab library we isolated a large number of mAbs that bind to the surface of human tumor cells. The mAbs were individually screened by immunostaining, and clones that ...

journal_title：Journal of immunological methods

authors： Kurosawa G,Sumitomo M,Akahori Y,Matsuda K,Muramatsu C,Takasaki A,Iba Y,Eguchi K,Tanaka M,Suzuki K,Morita M,Sato N,Sugiura M,Sugioka A,Hayashi N,Kurosawa Y

更新日期：2009-12-31 00:00:00

abstract：:Antigen-specific lymphocytes are important in the immune response to viral infection. Peripheral blood mononuclear cells (PBMC) are traditionally used as a source of effector cells in most immunological studies. We described here the use of the bispecific monoclonal antibodies (BSMAB) anti CD3:CD8 (CD3,8) and anti CD3...

journal_title：Journal of immunological methods

authors： Jones N,Agrawal D,Elrefaei M,Hanson A,Novitsky V,Wong JT,Cao H

更新日期：2003-03-01 00:00:00

abstract：:An assay has been developed in which the degree of in vitro spleen cell-mediated sheep red blood cell (SRBC) hemolysis is quantitated spectrophotometrically (QHS). Immune mouse spleen cells, SRBC and guinea pig complement were incubated at 37 degrees C for 1 h in buffer, centrifuged, and the supernatant solutions read...

journal_title：Journal of immunological methods

authors： Simpson MA,Gozzo JJ

更新日期：1978-01-01 00:00:00

abstract：:Western blotting is one of the most commonly and widely used methods for identifying the presence of an antigen in biochemical studies. The present study describes a novel 'coupled blotting' approach that allows antigen on dot and Western blots to be simultaneously probed with primary and secondary probing reagents. T...

journal_title：Journal of immunological methods

authors： Sundaram P

更新日期：1996-09-09 00:00:00

abstract：:A simple and sensitive liposome immunoassay for gentamicin was developed using the cytolytic agent, phospholipase C, instead of complement. Liposomes entrapping a fluorescent marker, calcein, were prepared by the reverse-phase evaporation method from a mixture of dimyristoylphosphatidylcholine and cholesterol (molar r...

journal_title：Journal of immunological methods

authors： Kim CK,Park KM

更新日期：1994-04-15 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for determination of antibodies against the zona pellucida was developed and compared with the already available indirect immunofluorescence (IIF) technique. Sera from 100 women with explained and unexplained infertility were screened for the presence of autoantibodies to t...

journal_title：Journal of immunological methods

authors： Singh J,Mhaskar AM

更新日期：1985-05-10 00:00:00

abstract：:An improved microassay for quantitation of murine macrophage-mediated antibody-dependent cellular cytotoxicity (ADCC) has been developed. The method is based on the turbidimetric measurement of sheep erythrocyte or nucleated (L1210) target cell suspensions at 630 nm with an automatic microtiter plate densitometer. The...

journal_title：Journal of immunological methods

authors： Rummage JA,Chang NS,Leu RW

更新日期：1986-01-22 00:00:00

abstract：:The identification of T cell epitopes that elicit a weak T cell response is technically challenging due to the relatively low resolution of many available screening assays. Peptide immunization can confirm the immunogenicity of a given peptide, however, this also often induces a low frequency response in lymphoid orga...

journal_title：Journal of immunological methods

authors： Usherwood EJ

更新日期：2002-08-01 00:00:00

abstract：:An in situ immunoassay technique is described which is sensitive to specific binding to surface immobile antigen or antibody. The phenomena of surface plasmon oscillations are shown to be sensitive to antigen-antibody binding on a substrate. An advantage of the technique is that no molecular labeling is required. The ...

journal_title：Journal of immunological methods

authors： Mayo CS,Hallock RB

更新日期：1989-06-02 00:00:00

abstract：:The human serine proteases granzymes A and B are expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise specific mouse monoclonal antibodies. Seven monoclonal antibodies (...

journal_title：Journal of immunological methods

authors： Kummer JA,Kamp AM,van Katwijk M,Brakenhoff JP,Radosević K,van Leeuwen AM,Borst J,Verweij CL,Hack CE

更新日期：1993-07-06 00:00:00

abstract：:The detection of specifically internal immunoglobulin by staining of fixed preparations of lymphoid cells with appropriate fluorescein-conjugated antisera can be hampered by binding of the conjugates to membrane-bound immunoglobulin. Without preventing staining through membrane-bound immunoglobulin, it is impossible ...

journal_title：Journal of immunological methods

authors： Mayrhofer G

更新日期：1979-01-01 00:00:00

abstract：:The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect different pathophysio...

journal_title：Journal of immunological methods

authors： Rasmussen KJ,Skjoedt MO,Vitved L,Skjoedt K,Palarasah Y

更新日期：2017-05-01 00:00:00

abstract：:A polyethylene glycol (PEG) radioimmunoprecipitation assay for human IgG is described that is sufficiently sensitive to detect 0.5 ng of IgG. This model antibody-antigen system was also used to study the stoichiometries of PEG-precipitated complexes. Our results suggest that the presence of PEG may affect the stoichio...

journal_title：Journal of immunological methods

authors： Waller SJ,Taylor RP,Andrews BS

更新日期：1979-01-01 00:00:00

abstract：:Intracellular DNA triggers interferon release during the innate immune response. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation ...

journal_title：Journal of immunological methods

authors： Miyakawa S,Okui T,Shiraishi T,Yoshihara T,Hirayama M,Satomi Y,Hamada T,Nishida M,Akimoto C,Sato S

更新日期：2020-05-01 00:00:00

abstract：:The mouse anti-human CD3 monoclonal antibody OKT3 is a potent immunosuppressive agent used in clinical transplantation. However, OKT3 therapy is associated with unpleasant and often serious side effects which appear to result from cytokine release, complement activation and a human anti-mouse antibody (HAMA) response....

journal_title：Journal of immunological methods

authors： Le Gall F,Reusch U,Moldenhauer G,Little M,Kipriyanov SM

更新日期：2004-02-01 00:00:00

abstract：:The sandwich solid phase radioimmunoassay procedure has been adapted to the serological diagnosis of tuberculosis by the use of disposable plastic haemagglutination trays in which the wells were sensitized with soluble mycobacterial antigen and the subsequent uptake of globulin from test sera detected with radioiodina...

journal_title：Journal of immunological methods

authors： Nassau E,Parsons ER,Johnson GD

更新日期：1975-01-01 00:00:00

abstract：:In this report the use of surface-linkage to expand the potential experimental and therapeutic applications of single chain antibody (scFv) constructs is reviewed. A strategy for the generation and functional characterization of surface-linked scFvs that bind selectively to the T-cell proteins CD3epsilon, CD28, and CD...

journal_title：Journal of immunological methods

authors： Griffin MD,Holman PO,Tang Q,Ashourian N,Korthäuer U,Kranz DM,Bluestone JA

更新日期：2001-02-01 00:00:00

abstract：:Many normal and pathological processes have been shown to be influenced by the quantitative and qualitative nature of cytokine production. Cytokines have also been shown to be central in modulating host responses to invasion by pathogens. Therefore detection and analysis of cytokine production are critical in the unde...

journal_title：Journal of immunological methods

authors： Walker KB

更新日期：1998-03-01 00:00:00