Abstract:
:Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial infections during the time, also reducing the cost. In this study, from random M13 phage display libraries, we selected phage clones that specifically bind surface of Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli. Then, commercial magnetic beads were functionalized with phage clones through covalent bond and used as capture and concentrating of pathogens from blood. We found that phage-magnetic beads complex represents a network which enables a cheap, high sensitive and specific detection of the bacteria involved in sepsis by micro-Raman spectroscopy. The enter process required 6 h and has the limit of detection of 10 Colony Forming Units on 7 ml of blood (CFU/7 ml).
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
De Plano LM,Fazio E,Rizzo MG,Franco D,Carnazza S,Trusso S,Neri F,Guglielmino SPPdoi
10.1016/j.jim.2018.12.004subject
Has Abstractpub_date
2019-02-01 00:00:00pages
45-52eissn
0022-1759issn
1872-7905pii
S0022-1759(18)30386-7journal_volume
465pub_type
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