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A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product.

Abstract：

:The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect different pathophysiological mechanisms. We have developed a rat antihuman C3d monoclonal antibody with specificity to the end sequence of the N-terminal region of C3d. The antibody can therefore only bind to C3d when it manifests itself as the final end product of cleaved C3. We believe that this specificity is it first of its kind, and predicts that it can be used as a detection tool in several immunological methods with great value in diagnostics.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Rasmussen KJ,Skjoedt MO,Vitved L,Skjoedt K,Palarasah Y

doi

10.1016/j.jim.2017.02.002

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

51-55

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(16)30282-4

journal_volume

444

pub_type

杂志文章

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