Abstract:
:Electroporation-based mono-gene therapy has received great interest in recent years but coadministration of different therapeutic genes for treatment of tumors has not been well explored. We hypothesize that electroporation is capable of delivering multiple genes that induce an additive or synergistic antitumor effect. To test this hypothesis, we used mice that were bearing SCCVII or TRAMP tumors. Established tumors with a diameter of 4-5 mm were injected with control plasmid DNA or plasmid DNA encoding B7.1, IL-12 or both via electroporation. Tumor regression, CTL activity and the level of B7.1, IL-12 and Stat1 expression were determined in both wild-type mice and in mice with a knock-out of the Stat1 gene. Remarkably, a single coadministration of the plasmids that encoded IL-12 and B7.1 eradicated tumors in 80% of mice. The therapeutic effect was associated with high levels of endogenous B7.1 expression, activity of cytotoxic lymphocytes, and activation of Stat1. Both exogenous B7.1 and IL-12 were required for inducing a high level of Stat1 activation in tumors, which occurred through a mechanism that was independent of the host Stat1. Both stimulators were also required for inducing the strong cytotoxic lymphocyte activity and for increasing the level and extending the duration of endogenous B7.1 expression. We therefore propose a 2-signal stimulation model to explain the synergistic effect of the coadministration of IL-12 and B7.1 on the regression of the tumors.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Liu J,Xia X,Torrero M,Barrett R,Shillitoe EJ,Li Sdoi
10.1002/ijc.22100subject
Has Abstractpub_date
2006-11-01 00:00:00pages
2113-8issue
9eissn
0020-7136issn
1097-0215journal_volume
119pub_type
杂志文章abstract::Studies of the MLTI were performed in a primary MSV-induced tumor system. Reactivity against mitomycin-C-treated RBL-5 cells could be detected during a limited period at about 14 days after virus injection. The degree of reactivity of MSV 14 spleen cells was quite variable; stimulation indices ranged from 2.2 to 9.4. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170313
更新日期:1976-03-15 00:00:00
abstract::Initial studies have investigated the association between inflammation and colorectal cancer (CRC) using C-reactive protein (CRP) as a proinflammatory biomarker and have noted inconsistent results among women. We here report the findings from a large prospective study with repeat measurements of CRP, as well as serum ...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
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abstract::An investigation was carried out to establish whether the reduction in catalase, glutathione peroxidase and superoxide dismutase activity, normally observed in liver tumours, is an early event and therefore of pathogenetic importance, or whether it is a late occurrence. Experiments performed on dimethylnitrosamine-tre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420529
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abstract::Marked variation in phenotypic expression among BRCA1 and BRCA2 mutation carriers may be partly explained by modifier genes that influence mutation penetrance. Variation in CAG/CAA repeat lengths coding for stretches of glutamines in the C-terminus of the AIB1 protein (amplified in breast cancer 1, a steroid receptor ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21176
更新日期:2005-11-01 00:00:00
abstract::We have investigated the effect of pre-treatment with the anti-cancer drugs cisplatin and etoposide on the susceptibility of P815 murine mastocytoma cells to lysis by murine spleen-derived anti-CD3-activated killer-T (AK-T) cells. A 20 hr pre-treatment with cisplatin (0.2-2 microg/ml) or etoposide (0.01-1 microg/ml) r...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971104)73:3<416::aid-ijc
更新日期:1997-11-04 00:00:00
abstract::The mounting of a specific immune response against the human papillomavirus type 16 E7 protein (HPV16 E7) is important for eradication of HPV16 E7-expressing cancer cells from the cervical mucosa. To induce a mucosal immune response by oral delivery of the E7 antigen, we expressed the HPV16 E7 antigen on the surface o...
journal_title:International journal of cancer
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doi:10.1002/ijc.22035
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abstract::There is good evidence for an association between Epstein-Barr virus (EBV) and Hodgkin's disease (HD). In approximately one-third of cases, the EBV genome is detectable in Reed-Sternberg (RS) cells and there is expression of the viral nuclear antigen EBNA-1 and the latent membrane protein LMP-1. Expression of LMP-2 ha...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970807)72:4<614::aid-ijc
更新日期:1997-08-07 00:00:00
abstract::We obtained Thy-I-positive cells directly from growing methylcholanthrene-induced (MCA-1510) sarcomas using fluorescence-activated cell sorting, then cultured these lymphocytes in medium containing Interleukin-2 and tested their activity in vivo against various MCA-sarcoma lines with the Winn assay. We found that cult...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280513
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abstract::Epigenetic modification of gene expression plays an important role in the development of human cancers. The inactivation of SPARC through CpG island methylation was studied in colon cancers using oligonucleotide microarray analysis and methylation specific PCR (MSP). Gene expression of 7 colon cancer cell lines was ev...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22706
更新日期:2007-08-01 00:00:00
abstract::The relation between physical activity and renal cell cancer is unclear. High occupational physical activity has been associated with a decreased risk of renal cell cancer among men-but not among women-in two previous studies, while no association has been found for leisure time physical activity. Our aim was to inves...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
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abstract::Chronic gastritis caused by Helicobacter pylori (H. pylori) infection could lead to the development of gastric cancer. The finding that multiple gastric cancers can develop synchronously and/or metachronously suggests the development of field cancerization in chronically inflamed, H. pylori-infected gastric mucosa. Th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31578
更新日期:2018-10-15 00:00:00
abstract::A hospital-based case-control study of renal-cell carcinoma was conducted in France from 1987 to 1991. A total of 196 histologically confirmed cases (138 males and 58 females) and 347 controls (235 males and 112 females), matched for sex, age at interview, hospital and interviewer, were included. The risk of renal-cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550107
更新日期:1993-08-19 00:00:00
abstract::Intestinal mucositis is a common side effect of chemotherapy leading to diarrhea, abdominal pain and increased risk of infections. The intestinal microbiota has been recognized as a key regulator of mucosal immune responses. Therefore, we hypothesized that intestinal microbial changes would be associated with enterocy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32942
更新日期:2020-10-01 00:00:00
abstract::Recent analyses have suggested decreases over time in colorectal cancer incidence at older ages (≥55 years) but increases at younger ages (20-54 years). Understanding the geographic heterogeneity of incidence facilitates resource allocation for potential interventions and advances our knowledge of differential etiolog...
journal_title:International journal of cancer
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doi:10.1002/ijc.31776
更新日期:2019-04-15 00:00:00
abstract::The identification of high-risk patients with thyroid cancer and the preoperative differentiation between follicular adenoma and carcinoma remain clinically challenging. Our study was conducted to analyze whether the quantification of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator receptor (...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24462
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abstract::This paper deals with a spontaneous malignant transformation in one of our XP fibroblast lines. This cell line, designated XP29MA, was derived from a 14-year-old boy who did not show skin tumors or precancerous alterations either at the time of clinical examination or when the biopsy was taken. We have compared the fo...
journal_title:International journal of cancer
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更新日期:1983-06-15 00:00:00
abstract::Interleukin-12 (IL-12) has potent antitumor activities via natural killer cells and cytotoxic T lymphocytes. However, the molecular mechanisms whereby IL-12 induces tumoricidal activities are poorly understood. Here, we report the genome-wide analysis of gene expression in a primary murine mammary carcinoma model that...
journal_title:International journal of cancer
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doi:10.1002/ijc.20145
更新日期:2004-07-01 00:00:00
abstract::Tumor cells express the glycolytic regulator pyruvate kinase subtype M2 (M2-PK), which can occur in a tetrameric form with high affinity to its substrate phosphoenolpyruvate (PEP) and a dimeric form with a low PEP affinity. The transition between both conformations contributes to the control of glycolysis and is impor...
journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:2008-07-15 00:00:00
abstract::To investigate the genetic changes that occur during establishment of gastric cancer cell lines, 4 gastric cancer cell lines and their original tumor tissues were examined for microsatellite instability (MSI), loss of heterozygosity (LOH), and p53 mutation. MSI status did not change during the establishment, though th...
journal_title:International journal of cancer
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doi:10.1002/1097-0215(20000815)87:4<512::aid-ijc8>3.0.
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abstract::Peripheral blood T cells from a patient with T-cell chronic lymphocytic leukemia (T-CLL) failed to respond to mitogenic lectins or alloantigens but could be induced to proliferate by the addition of exogenous interleukin-2 (IL-2). The T-CLL cells also proliferated in response to 12-O-tetradecanoyl phorbol-13-acetate (...
journal_title:International journal of cancer
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abstract::It is considered that fruit and vegetable (F&V) protect against oesophagus and gastric cancer (GC). However, 2 recent meta-analyses suggest that the strength of association on GC seems to be weaker for vegetables than for fruit and weaker in cohort than in case-control studies. No evidence exists from cohort studies a...
journal_title:International journal of cancer
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journal_title:International journal of cancer
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abstract::We have previously shown that anti-Ly-6C monoclonal antibody (MAb) 2B6-F2 identifies a subset of (CBA +/- C57BL/6)F1 splenic NK-1.1+ natural T (NT) cells which kill the NK-sensitive YAC-1 target in vitro. Furthermore, these Ly-6C+ cells are responsible for 40-50% of in vitro YAC-1 killing in all mouse strains tested. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960516)66:4<532::AID-IJC
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971009)73:2<240::aid-ijc
更新日期:1997-10-09 00:00:00
abstract::A novel non-phorbol-ester-like tumor promoter, okadaic acid (OA) has been shown to be an inhibitor of protein phosphatase I and IIA and, thus, to cause an "apparent activation" of protein kinase C (PKC). We previously showed that cis-diamminedichloroplatinum(II) (CDDP)-resistant cells, PC-9/CDDP, were cross-resistant ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500319
更新日期:1992-02-01 00:00:00
abstract::Hypoxia is known to play important roles in the development and progression of tumors. We previously demonstrated that S100A4, a critical molecule for metastasis, was upregulated in ovarian cancer cells. Therefore, we examined the mechanisms of the upregulation of S100A4 expression in ovarian carcinoma cells, with par...
journal_title:International journal of cancer
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更新日期:2012-10-15 00:00:00
abstract::Human blood monocytes are very potent to take up antigens. Like macrophages in tissue, they efficiently degrade exogenous protein and are less efficient than dendritic cells (DCs) at cross-presenting antigens to CD8+ T cells. Although it is generally accepted that DCs take up tissue antigens and then migrate to lymph ...
journal_title:International journal of cancer
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更新日期:2018-01-01 00:00:00
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journal_title:International journal of cancer
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abstract::Pathological deregulation of the transforming growth factor, beta 1 (TGFB1) pathway has been implicated in the development of several major diseases, including cancers. Regulatory variation in the TGFB1 gene may lead to altered TGFB1 expression and activity, and thus, modulate an individual's susceptibility to disease...
journal_title:International journal of cancer
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更新日期:2009-09-15 00:00:00
abstract::The Swedish Family-Cancer Database was used to analyze second cancers after oral, esophageal, rectal, cervical, genital and skin (squamous cell carcinoma) cancers. A strong and consistent association of second cancers was observed at all these sites, in men and women. As a novel finding, an association of rectal cance...
journal_title:International journal of cancer
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