Abstract：
:The mounting of a specific immune response against the human papillomavirus type 16 E7 protein (HPV16 E7) is important for eradication of HPV16 E7-expressing cancer cells from the cervical mucosa. To induce a mucosal immune response by oral delivery of the E7 antigen, we expressed the HPV16 E7 antigen on the surface of Lactobacillus casei by employing a novel display system in which the poly-gamma-glutamic acid (gamma-PGA) synthetase complex A (PgsA) from Bacillus subtilis (chungkookjang) was used as an anchoring motif. After surface expression of the HPV16 E7 protein was confirmed by Western blot, flow cytometry and immunofluorescence microscopy, mice were orally inoculated with L. casei-PgsA-E7. E7-specific serum IgG and mucosal IgA productions were enhanced after oral administration and significantly enhanced after boosting. Systemic and local cellular immunities were significantly increased after boosting, as shown by increased counts of lymphocytes (SI = 9.7 +/- 1.8) and IFN-gamma secreting cells [510 +/- 86 spot-forming cells/10(6)cells] among splenocytes and increased IFN-gamma in supernatants of vaginal lymphocytes. Furthermore, in an E7-based mouse tumor model, animals receiving orally administered L. casei-PgsA-E7 showed reduced tumor size and increased survival rate versus mice receiving control (L. casei-PgsA) immunization. These results collectively indicate that the oral administration of E7 displayed on lactobacillus induces cellular immunity and antitumor effects in mice.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Poo H,Pyo HM,Lee TY,Yoon SW,Lee JS,Kim CJ,Sung MH,Lee SHdoi
10.1002/ijc.22035subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1702-9issue
7eissn
0020-7136issn
1097-0215journal_volume
119pub_type
杂志文章abstract：:Recent genome-wide scans identified several novel breast cancer risk alleles, including variants of the FGFR2, MAP3K1 and LSP1 genes, and a study of associations between these alleles and characteristics of breast cancer patients reported a borderline significant correlation between the number of FGFR2 minor alleles a...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23924
更新日期：2009-02-01 00:00:00
abstract：:The effects on breast cancer mortality seen after 16 years of biennial screening of younger women are assessed in this prospective cohort study. Since 1975 some 13,500 women, aged 35-49 in 1975, were invited to participate in the Nijmegen screening programme comprising a mammographic examination every 2 years. By the ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910600614
更新日期：1995-03-16 00:00:00
abstract：:Although the incidence of, and deaths due to, malignant melanoma are rising at a rapid rate, few experimental models mimic the highly metastatic properties associated with the pathogenesis of the human disease, making study of the disease difficult. Thus, new human models are required to understand melanoma biology, e...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910470211
更新日期：1991-01-21 00:00:00
abstract：:Angiogenesis and immunosuppression work hand-in-hand in the renal cell carcinoma (RCC) microenvironment. Tumor growth is associated with impaired antitumor immune response in RCC, which involves T cells, natural killer cells, dendritic cells (DCs) and macrophages. Vascular endothelial growth factor receptor (VEGFR), s...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.28503
更新日期：2014-06-15 00:00:00
abstract：:Cytogenetic deletions of the short arm of chromosome 9, 9p, have been detected in cell lines of malignant mesothelioma as well as in tumor material. Many tumor types carry deletions of chromosome 9 or more specifically of 9p21. The tumor-suppressor genes, CDKN2A and CDKN2B, each of which encodes a structurally and fun...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980209)75:4<649::aid-ijc
更新日期：1998-02-09 00:00:00
abstract：:Cytogenetic analysis of short-term cultures from 26 primary bronchial large cell carcinomas and 1 metastasis from a primary large cell carcinoma revealed clonal chromosome abnormalities in 20 tumors and a normal karyotype in 6. No outgrowth was obtained in 1 case. Simple aberrations were present in 3 tumors; in 1, the...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910570404
更新日期：1994-05-15 00:00:00
abstract：:Using individual subject data from 10 case-control studies, comprising over 3000 cases and almost 4000 controls, we have estimated the relative risk of melanoma associated with aspects of complexion, namely, hair, eye and skin colour and freckling in adulthood, and have examined the relationships between these factors...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910620402
更新日期：1995-08-09 00:00:00
abstract：:The influence of polyunsaturated fatty acids (PUFAs) on risk of colorectal cancer precursors remains largely unknown. We examined the associations of erythrocyte PUFAs, including n-3 and n-6 PUFAs, with risk of colorectal conventional adenomas and serrated polyps in 4517 participants from three US prospective cohorts ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.33190
更新日期：2021-01-01 00:00:00
abstract：:The incidence of esophageal cancer is extremely high in Linxian County and certain other regions of the People's Republic of China. Epidemiologic and laboratory studies suggest that N-nitroso carcinogens and other environmental factors play a causative role. In the present study, employing over 100 DNA samples obtaine...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910420406
更新日期：1988-10-15 00:00:00
abstract：:Human papilloma virus is a causative factor in the etiology of cervical cancer with HPV16 being the most prevalent genotype associated with it. Intratype variations in oncogenic E6/E7 and capsid L1 proteins of HPV 16 besides being of phylogenetic importance, are associated with risk of viral persistence and progressio...
journal_title：International journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1002/ijc.24322
更新日期：2009-07-01 00:00:00
abstract：:In order to update the findings of relative risk associated with cigarette smoking for lung cancer by histologic type in Japan, the data from a population-based cohort study of 91,738 men and women were analyzed. During 1990-1999, 422 lung cancer incident cases were identified. The relative risk for all incident cases...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.10308
更新日期：2002-05-10 00:00:00
abstract：:We have quantified the expression of all 4 isoforms of vascular endothelial growth factor (VEGF) mRNA in non-small-cell lung cancer (NSCLC) using a new kinetic quantitative PCR method, real-time quantitative (RTQ) RT-PCR, and investigated the association between VEGF expression at the mRNA and protein levels and the c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/1097-0215(20001120)89:6<475::aid-ijc2>3.0.
更新日期：2000-11-20 00:00:00
abstract：:EPLIN is frequently downregulated or lost in various cancers. The purpose of this study was to evaluate the importance of EPLIN in prostate cancer progression, with particular focus on the mechanistic implications to elucidate EPLIN's tumor suppressive function in cancer. EPLIN expression was evaluated in prostate can...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31786
更新日期：2018-11-15 00:00:00
abstract：:The Swedish Family-Cancer Database was used to analyze concordant (same site) and discordant (different site) second primary neoplasms in 633,964 cancer patients diagnosed from 1958 to 1996. Cases of second malignant neoplasms were extracted from the Database if the diagnosis date of the first and second cancer differ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.1317
更新日期：2001-07-15 00:00:00
abstract：:This study was aimed at comparing screen-detected and symptomatic breast carcinomas with regard to the prevalence of positive immunostaining for c-erb-B-2 oncoprotein. Paraffin sections of 81 breast carcinomas detected through the National Screening Programme were examined for the over-expression of c-erbB-2 oncoprote...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910550112
更新日期：1993-08-19 00:00:00
abstract：:Suramin has been shown to have antiproliferative activity, either by blocking the binding of growth factors to their receptors or by inhibiting critical cellular enzymes. In 6 different cell lines from 5 tumour types (MCF-7, MCF-7/ADRR, PC3, HT-29, UM-SCC-11B and SW-1573/IR500), we studied the effect of scheduling of ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910510615
更新日期：1992-07-30 00:00:00
abstract：:In normal somatic cells, the methylation pattern of DNA is stably maintained by DNA (cytosine-5-)-methyltransferase (DNA methyltransferase). Increased expression of DNA methyltransferase has been detected in many types of human cancer and has been thought to play an important role in tumorigenesis. In our study, we de...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19990924)83:1<10::aid-ijc3
更新日期：1999-09-24 00:00:00
abstract：:Serum samples from feral populations of African green monkeys (Cercopithecus aethiops) were screened for antibodies to the simian T-lymphotropic virus, type I (STLV-I). Blood samples had been collected from 336 monkeys in 4 regions of central and southern Kenya in 1978 and 1979, from 114 monkeys in central Ethiopia in...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910380412
更新日期：1986-10-15 00:00:00
abstract：:Tyrosine kinase inhibitors are effective treatments for cancers. Knowing the specific kinase mutants that drive the underlying cancers predict therapeutic response to these inhibitors. Thus, the current protocol for personalized cancer therapy involves genotyping tumors in search of various driver mutations and subseq...
journal_title：International journal of cancer
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更新日期：2018-01-01 00:00:00
abstract：:Data on the incidence of childhood cancer in Queensland during the 10-year period 1979-88 are presented. During this period, 786 cases were registered. The average annual crude and age-standardized (to the world population) incidence rates for both sexes were 12.63 and 13.30 per 100,000 respectively. The incidence of ...
journal_title：International journal of cancer
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更新日期：1990-06-15 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) is a cell plasticity process required for metastasis and chemoresistance of carcinoma cells. We report a crucial role of the signal adaptor proteins CRK and CRKL in promoting EMT and tumor aggressiveness, as well as resistance against chemotherapy in colorectal and pancreatic ca...
journal_title：International journal of cancer
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abstract：:The association between subtypes of hepatitis C virus (HCV) and risk of hepatocellular carcinoma (HCC) remained inconclusive and evaluated in both case-control and cohort studies. In the case-control study, 397 HCC cases from medical centers were compared with 410 community-based non-HCC controls. All of them were ant...
journal_title：International journal of cancer
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更新日期：2014-09-01 00:00:00
abstract：:The mode of transmission of human herpesvirus 8 (HHV8) was investigated in two seroepidemiological studies of Swedish women who completed a questionnaire about sexual behavior. Seropositivity for HHV8 antibodies, measured using an indirect immunofluorescence assay, was linked to a high number (>10) of sexual partners ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：
更新日期：2000-07-15 00:00:00
abstract：:We previously determined that human papillomavirus (HPV) virus-like particles (VLPs) and pseudovirions (PsV) did not, respectively, bind to or infect intact epithelium of the cervicovaginal tract. However, they strongly bound heparan sulfate proteoglycans (HSPG) on the basement membrane of disrupted epithelium and inf...
journal_title：International journal of cancer
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更新日期：2016-02-15 00:00:00
abstract：:Neuron-specific src mRNA, which is expressed in human brain tissue by alternative splicing, is associated with neural differentiation. Neuronal c-srcNI expression may be associated with the ability of neuroblastomas to mature; furthermore, c-srcN2 mRNA is induced in chemically differentiated neuroblastoma cells in vit...
journal_title：International journal of cancer
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更新日期：1994-09-15 00:00:00
abstract：:The aim of our study was to further explore the use of anti-angiogenic therapy targeting the vascular endothelial growth factor receptor (VEGFR) on endothelial cells while simultaneously targeting platelet-derived growth factor receptors (PDGFRs) on adjacent pericytes. B16 mouse melanoma tumors exogenously expressing ...
journal_title：International journal of cancer
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更新日期：2007-12-15 00:00:00
abstract：:The role of spontaneous and induced abortion on breast cancer risk is examined among 267,361 women recruited into the European Prospective Investigation into Cancer and nutrition between 1992 and 2000. The data were collected from 20 centers, across 9 countries, and included information on a total of 4,805 women with ...
journal_title：International journal of cancer
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abstract：:Transforming growth factor-beta (TGF-beta) signaling is initiated following heterodimerization of the type II TGF-beta receptor (TbetaRII) with the type I TGF-beta receptor (TbetaRI). Both receptors are required for TGF-beta responsiveness. In the present study, we characterized the actions of TGF-beta1 in T3M4 human ...
journal_title：International journal of cancer
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abstract：:The role of alcoholic beverages in the etiology of gastric cancer is unclear. Recent summaries showed a positive association between higher alcohol intake and gastric cancer risk, but the magnitude of association is small, there is moderate heterogeneity among studies, and most cases were from Asian populations. We pr...
journal_title：International journal of cancer
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abstract：:The incidence of cutaneous melanoma in children aged 0-14 years was examined in Queensland, Australia. Details of notifications were collected from the population-based Australian Paediatric Cancer Registry. Between 1987 and 1994, the age-adjusted incidence rates of invasive cutaneous melanoma occurring in Queensland ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910630602
更新日期：1995-12-11 00:00:00
