Genetic alterations in gastric cancer cell lines and their original tissues.

Abstract:

:To investigate the genetic changes that occur during establishment of gastric cancer cell lines, 4 gastric cancer cell lines and their original tumor tissues were examined for microsatellite instability (MSI), loss of heterozygosity (LOH), and p53 mutation. MSI status did not change during the establishment, though the cell lines gained frameshift mutation in some of the genes with polytract coding sequences. There was no difference in p53 mutation between the cell lines and the original tumors. The frequency of LOH was similar between the cell lines and each original tumor, in the range 30.8% to 85.7%. The difference in the LOH results between the cell lines and the original tumors was not greater than the difference between the different areas of the original tumors. The above results suggested that most of the genetic alterations, such as MSI, LOH, and p53 gene mutation, were sustained during the establishment of gastric cancer cell lines. Minor genetic differences between original tumor tissues and cancer cell lines could be explained as a tumor heterogeneity because separate areas of the original tumor tissues manifest similar variations.

journal_name

Int J Cancer

authors

Bae SI,Park JG,Kim YI,Kim WH

doi

10.1002/1097-0215(20000815)87:4<512::aid-ijc8>3.0.

keywords:

subject

Has Abstract

pub_date

2000-08-15 00:00:00

pages

512-6

issue

4

eissn

0020-7136

issn

1097-0215

pii

10.1002/1097-0215(20000815)87:4<512::AID-IJC8>3.0.

journal_volume

87

pub_type

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