Abstract:
:KDR (kinase insert domain receptor) phosphorylation induces several effects which lead eventually to cell proliferation and survival. The precise mechanisms by which KDR, once it is activated, communicates with the nucleus are starting to be understood but have not yet been completely unravelled. Two in vitro studies on animal cell lines reported in the literature have demonstrated that, following stimulation with VEGF, KDR is actually translocated within the nucleus. Our aim was to investigate whether this translocation occurs in human cells both in vitro and in vivo. Using laser scanning confocal microscopy, a variable nuclear localization of phosphorylated and total KDR in cell lines and tumour samples was found. In human neoplastic cell lines, hypoxic stimulation greatly increased the nuclear amount of total KDR but less so that of the phosphorylated form. Only after hypoxia and VEGF stimulation there was a comparably increased expression of phosphorylated and total KDR observed in the nuclei of these cells. We conclude that neoplastic cells show a variable expression of total and phosphorylated KDR in the nucleus. The precise functional meaning of nuclear location remains to be established.
journal_name
Cell Resjournal_title
Cell researchauthors
Blazquez C,Cook N,Micklem K,Harris AL,Gatter KC,Pezzella Fdoi
10.1038/sj.cr.7310012keywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
93-8issue
1eissn
1001-0602issn
1748-7838pii
7310012journal_volume
16pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract::While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2 ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-018-0113-8
更新日期:2019-01-01 00:00:00
abstract::Differentiation of inflammatory macrophages from monocytes is characterized by an orderly integration of epigenetic and transcriptional regulatory mechanisms guided by lineage-determining transcription factors such as PU.1. Further activation of macrophages leads to a stimulus- or microenvironment-specific signal inte...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.1
更新日期:2016-02-01 00:00:00
abstract::The term epigenetics refers to heritable changes not encoded by DNA. The organization of DNA into chromatin fibers affects gene expression in a heritable manner and is therefore one mechanism of epigenetic inheritance. Large parts of eukaryotic genomes consist of constitutively highly condensed heterochromatin, import...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2009.13
更新日期:2009-03-01 00:00:00
abstract::A wide variety of neurons and glial cells differentiate from common precursor cells in the developing nervous system. During this process, Notch-mediated cell-cell interaction is essential for maintenance of dividing cells and subsequent generation of cell type diversity. Activation of Notch inhibits cellular differen...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290016
更新日期:1999-09-01 00:00:00
abstract::We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspension-cultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA fragments. The effects of different divalent cations and cycloheximide on DNA cleavage in this system ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290008
更新日期:1999-06-01 00:00:00
abstract::In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression subtractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88 genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, inc...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290127
更新日期:2002-09-01 00:00:00
abstract::The TGF-beta superfamily members have important roles in controlling patterning and tissue formation in both invertebrates and vertebrates. Two types of signal transducers, receptors and Smads, mediate the signaling to regulate expression of their target genes. Despite of the relatively simple signal transduction path...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290246
更新日期:2004-12-01 00:00:00
abstract::The human CD14(+) monocyte compartment is composed by two subsets based on CD16 expression. We previously reported that this compartment is perturbed in tuberculosis (TB) patients, as reflected by the expansion of CD16(+) monocytes along with disease severity. Whether this unbalance is beneficial or detrimental to hos...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2015.123
更新日期:2015-12-01 00:00:00
abstract::While studying the neural precursor cell intermediate filament protein known as nestin in the developing mouse brain, we observed a strong cross-reaction of our nestin antibody with a 50 kDa protein that appeared on embryonic day 10 and continued to accumulate until postnatal day 1. Here we report evidence that this p...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290030
更新日期:1999-12-01 00:00:00
abstract::Long-range chromatin interactions between enhancers and promoters are essential for transcription of many developmentally controlled genes in mammals and other metazoans. Currently, the exact mechanisms that connect distal enhancers to their specific target promoters remain to be fully elucidated. Here, we show that t...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2018.1
更新日期:2018-02-01 00:00:00
abstract::Wor1 (white-opaque switching regulator 1) is a master regulator of the white-opaque switching in Candida albicans, an opportunistic human fungal pathogen, and is associated with its pathogenicity and commensality. Wor1 contains a conserved DNA-binding region at the N-terminus, consisting of two conserved segments (WOP...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2014.102
更新日期:2014-09-01 00:00:00
abstract::Oct4 is a well-known transcription factor that plays fundamental roles in stem cell self-renewal, pluripotency, and somatic cell reprogramming. However, limited information is available on Oct4-associated protein complexes and their intrinsic protein-protein interactions that dictate Oct4's critical regulatory activit...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.179
更新日期:2012-01-01 00:00:00
abstract::Elimination of misfolded proteins of the endoplasmic reticulum (ER) requires their retrotranslocation from the ER to the cytosol via membrane-bound ubiquitin ligase complexes. Baldridge and Rapoport now reconstitute a key step of retrotranslocation, demonstrating a protein conduit gated by ubiquitination. ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.92
更新日期:2016-10-01 00:00:00
abstract::Aging is associated with many complex diseases. Reliable prediction of the aging process is important for assessing the risks of aging-associated diseases. However, despite intense research, so far there is no reliable aging marker. Here we addressed this problem by examining whether human 3D facial imaging features c...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2015.36
更新日期:2015-05-01 00:00:00
abstract::Directional migration of leukocytes is indispensable to innate immunity for host defense. However, recruitment of leukocytes to a site of tissue injury also constitutes a leading cause for inflammatory responses. Mechanistically, it involves a cascade of cellular events precisely regulated by temporal and spatial pres...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290071
更新日期:2001-06-01 00:00:00
abstract::Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, bud2, which results from the complete deletion of one member of the small gene family that encodes S-a...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310056
更新日期:2006-05-01 00:00:00
abstract::Carotenoids, a class of natural pigments found in all photosynthetic organisms, are involved in a variety of physiological processes, including coloration, photoprotection, biosynthesis of abscisic acid (ABA) and chloroplast biogenesis. Although carotenoid biosynthesis has been well studied biochemically, the genetic ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2007.37
更新日期:2007-05-01 00:00:00
abstract::New plant type (NPT) or ideal plant architecture (IPA) is an attractive way of increasing yield potential by promoting high resource use efficiency combined with better lodging resistance. In a recent paper in Cell Research, Wang et al. describe how a QTL they identified could bring about the desired NPT architecture ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2017.115
更新日期:2017-10-01 00:00:00
abstract::This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for beta-glucuroni...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290092
更新日期:2001-09-01 00:00:00
abstract::There is strong relationship between melanocortin-1 receptor (MC1R) gene variants and human hair color and skin type. Based on a sequencing study of MC1R gene in 50 individuals from the Uygur, Tibetan, Wa and Dai ethnic populations, we discuss the occurrence of 7 mc1r variants consisting of 5 nonsynonymous sites (Val6...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290070
更新日期:2001-03-01 00:00:00
abstract::CCAAT/enhancer binding protein alpha (C/EBPalpha) is a transcriptional regulatory factor that inhibits cell proliferation, and alternative translational initiation produces two polypeptides, C/EBPalphap30 and C/EBPalphap42. By expression profiling, it was revealed that C/EBPalphap30 specifically inhibited a unique set...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310121
更新日期:2007-04-01 00:00:00
abstract::Brain tumors are dynamic complex ecosystems with multiple cell types. To model the brain tumor microenvironment in a reproducible and scalable system, we developed a rapid three-dimensional (3D) bioprinting method to construct clinically relevant biomimetic tissue models. In recurrent glioblastoma, macrophages/microgl...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-020-0338-1
更新日期:2020-10-01 00:00:00
abstract::UV-B (280-315 nm) is an integral part of solar radiation and can act either as a stress inducer or as a developmental signal. In recent years, increasing attention has been paid to the low-fluence UV-B-induced photomorphogenic response and several key players in this response have been identified, which include UVR8 (...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2012.34
更新日期:2012-06-01 00:00:00
abstract::Ruptured and intact plasma membranes are classically considered as hallmarks of necrotic and apoptotic cell death, respectively. As such, apoptosis is usually considered a non-inflammatory process while necrosis triggers inflammation. Recent studies on necroptosis and pyroptosis, two types of programmed necrosis, reve...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2017.133
更新日期:2018-01-01 00:00:00
abstract::In 2006, the "wall came down" that limited the experimental conversion of differentiated cells into the pluripotent state. In a landmark report, Shinya Yamanaka's group described that a handful of transcription factors (Oct4, Sox2, Klf4 and c-Myc) can convert a differentiated cell back to pluripotency over the course ...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2011.28
更新日期:2011-03-01 00:00:00
abstract::In order to analyze the mechanism of immuno-modulation by LPS on murine peritoneal suppressor macrophages, we have, using RNase protection assay, checked the changes of mRNA expression pattern of several cytokine genes during the immuno-modulation. It has been found that, after treating peritoneal suppressor macrophag...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.1998.32
更新日期:1998-12-01 00:00:00
abstract::Recent evidence indicates that codon optimality is a broad determinant of mRNA stability. A study by Radhakrishnan et al. in Cell raises the possibility that the conserved DEAD-box protein Dhh1 underlies the phenomenon. ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.127
更新日期:2016-12-01 00:00:00
abstract::Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig, namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290156
更新日期:2003-04-01 00:00:00
abstract::Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are s...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2008.282
更新日期:2008-10-01 00:00:00
abstract::Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290151
更新日期:2003-02-01 00:00:00