Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Abstract:

:Brucellosis is a worldwide zoonosis causing reproductive failures in livestock and a severe multi-organ disease in humans. The genus Brucella is divided into seven species and various biotypes differing in pathogenicity and host specificity. Although Brucella spp. represent a highly homogenous group of bacteria, RFLPs of selected genes display sufficient polymorphism to distinguish Brucella species and biovars. PCR-RFLP analysis shows excellent typeability, reproducibility, stability, and epidemiological concordance. Consequently, PCR-RFLP assays of specific gene loci can serve as tools for diagnostic, epidemiological, taxonomic, and evolutionary studies. Various PCR-RFLPs used for the identification of Brucella species and biotypes are reviewed.

journal_name

Crit Rev Microbiol

authors

Al Dahouk S,Tomaso H,Prenger-Berninghoff E,Splettstoesser WD,Scholz HC,Neubauer H

doi

10.1080/10408410500304041

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

191-6

issue

4

eissn

1040-841X

issn

1549-7828

journal_volume

31

pub_type

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