Abstract:
:Brucellosis is a worldwide zoonosis causing reproductive failures in livestock and a severe multi-organ disease in humans. The genus Brucella is divided into seven species and various biotypes differing in pathogenicity and host specificity. Although Brucella spp. represent a highly homogenous group of bacteria, RFLPs of selected genes display sufficient polymorphism to distinguish Brucella species and biovars. PCR-RFLP analysis shows excellent typeability, reproducibility, stability, and epidemiological concordance. Consequently, PCR-RFLP assays of specific gene loci can serve as tools for diagnostic, epidemiological, taxonomic, and evolutionary studies. Various PCR-RFLPs used for the identification of Brucella species and biotypes are reviewed.
journal_name
Crit Rev Microbioljournal_title
Critical reviews in microbiologyauthors
Al Dahouk S,Tomaso H,Prenger-Berninghoff E,Splettstoesser WD,Scholz HC,Neubauer Hdoi
10.1080/10408410500304041keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
191-6issue
4eissn
1040-841Xissn
1549-7828journal_volume
31pub_type
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