Abstract：
:Fibroblast growth factor 2 (FGF2) exhibits pleiotropic functions during embryogenesis. In neural development, both pro- and antineurogenic activities of FGF2 have been described in the differentiation of neuronal progenitors into postmitotic neurons. We used cultured neural stem cells (NSCs) derived from rat embryonic day 14.5 cortex to determine the FGF2 effect on the induction of early neuronal progenitors. Our data showed that the presence of FGF2 during serum-induced differentiation of NSCs reduced the number of Tuj1(+) neurons. A bromodeoxyuridine (BrdU)/Tuj1 double-labeling assay and expression analyses of the pro- and antineurogenic basic helix-loop-helix (bHLH) factors showed that FGF2 blocked the generation of early neuronal progenitors, but not the cell-cycle exit of dividing neurons. This negative regulation of neuronal induction by FGF2 was associated with the persistent expression of an antineurogenic bHLH, hairy and enhancer of split (HES)-1. A gene-profiling study demonstrated that the developmental programs underlying neuronal differentiation were altered as a whole and identified several developmentally regulated, neural-enriched genes. This work shows that FGF2 exerts an antineurogenic effect during the developmental window when neuronal progenitors are first induced from NSCs. It also provides a novel experimental system that can be used to prospectively identify genes expressed at different stages of neuronal differentiation.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Tsai RY,Kim Sdoi
10.1002/jnr.20627keywords:
subject
Has Abstractpub_date
2005-10-15 00:00:00pages
149-59issue
2eissn
0360-4012issn
1097-4547journal_volume
82pub_type
杂志文章abstract：:The effects of glutamate on intracellular free Ca2+, [Ca2+]i, and neurotoxicity were compared in cerebellar granule neurons in vitro. [Ca2+]i was measured with fura-2 and digital fluorescence imaging microscopy; neurotoxicity was monitored using a vital dye and colorimetric analysis. Glutamate produced dose-dependent ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490280317
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20743
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abstract：:Pharmacological characterization of the action of the novel non-N-methyl-D-aspartate (non-NMDA) antagonist AMOA (2-amino-3-[3-(carboxymethoxy)-5-methylisoxazol-4-yl]propionate) on glutamate receptors was investigated in Xenopus oocytes injected with mouse brain mRNA. AMOA (150 microM) produced a nearly parallel shift ...
journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490330305
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abstract：:To scale up human neural stem/progenitor cell (NSPC) cultures for clinical use, we need to know how long these cells can live ex vivo without losing their ability to proliferate and differentiate; thus, a convenient method is needed to estimate the proliferative activity of human NSPCs grown in neurosphere cultures, a...
journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490370310
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pub_type： 杂志文章,评审
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更新日期：2016-11-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10756
更新日期：2003-10-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：1996-01-15 00:00:00
abstract：:The incubation of murine thymocytes for 24 hr in the presence of cholinergic agonists induces selective proliferation of PNA- cells as measured by thymidine incorporation. The response of PNA- cells is mediated by the activation of nicotinic receptors. This conclusion is suggested by the inability of the muscarinic ag...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490240305
更新日期：1989-11-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21867
更新日期：2009-02-01 00:00:00
abstract：:Previous studies have shown that treating rat cortical neurons in primary culture with apolipoprotein E (apoE) peptide increased cytoplasmic Ca2+ by 2 mechanisms: 1) an influx of extracellular Ca2+ resulting from the activation of a cell surface Ca2+ channel; and 2) release of Ca2+ from internal Ca2+ stores via a G-pr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980301)51:5<658::AID-JNR
更新日期：1998-03-01 00:00:00
abstract：:Previous studies have shown that the mechanical wounding of 3-week-old cultured rat astrocytes results in cell proliferation and hypertrophy resembling astrocyte responses to a brain injury in vivo. We now report the effects of basic fibroblast growth factor (bFGF) and an anti-bFGF antibody on astrocyte morphology, pr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490400310
更新日期：1995-02-15 00:00:00
abstract：:Granulocyte colony-stimulating factor (G-CSF) and its related mechanisms were investigated to assess the potential for this factor to exert neuroprotective effects against spinal cord injury in mice. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was injected into mice spinal cord hemisection models...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23362
更新日期：2014-06-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10074
更新日期：2002-01-01 00:00:00
abstract：:Nicotine has been reported to be neuroprotective in experimental and epidemiological studies. In addition to nicotine, tobacco and cigarette smoke contain cembranoids, which are antagonists of neuronal nicotinic receptors (nAChR). Exposure of hippocampal slices to N-methyl-D-aspartate (NMDA) decreases the population s...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20666
更新日期：2005-12-01 00:00:00
abstract：:The phosphorylative neuromodulation of the regulatory subunit of protein kinase type II (R-II) in cytosolic fractions from denervated and sham-operated, contralateral soleus muscles of the rat was evaluated. The denervation-induced increase in the 32P-phosphorylation of R-II is not related to an increased dephosphoryl...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490140208
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abstract：:The neuron-glia cell adhesion molecule (Ng-CAM) mediates neuron-neuron adhesion by a homophilic mechanism and neuron-astrocyte adhesion by a heterophilic mechanism. The protein is expressed on neurons and Schwann cells but not on astrocytes. It is most prevalent during development on cell bodies of migrating neurons a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.490310102
更新日期：1992-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10694
更新日期：2003-09-01 00:00:00
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360408
更新日期：1993-11-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20329
更新日期：2004-12-15 00:00:00
abstract：:N-cadherin and beta-catenin are involved in cell adhesion and cell cycle in tumor cells and neural crest. Both are expressed at key stages of Schwann cell (SC) development, but little is known about their function in the SC lineage. We studied the role of these molecules in adult rat derived SC-embryonic dorsal root g...
journal_title：Journal of neuroscience research
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更新日期：2008-03-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490060403
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abstract：:Production of hydrogen peroxide as a by-product of the breakdown of catecholamines by the enzyme monoamine oxidase (MAO) has been hypothesized to contribute to the increased proclivity of dopaminergic neurons for oxidative injury. We established clonal dopaminergic PC12 cell lines which have elevated MAO activity leve...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19961215)46:6<666::AID-JNR
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abstract：:Recent findings suggest that exogenous gangliosides improve recovery of a learned behavior (alternation in a T-maze) which is thought to be related to sprouting after lesions of the entorhinal cortex. In the present investigation, we studied an unlearned behavior (the open-field hyperactivity resulting from bilateral ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490120228
更新日期：1984-01-01 00:00:00
abstract：:Cultures of chicken day 8 embryo retinal cells, essentially free of contaminating non-neuronal elements, were used to examine the neurotoxicity of various excitatory amino acid transmitter receptor agonists. At 7 days in vitro, N-methyl-D-aspartate (NMDA), following 24 hr exposure to 0.1-1.0 mM, destroyed 60-70% of th...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270210
更新日期：1990-10-01 00:00:00
abstract：:The effect of the potent soluble guanylyl cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) on neurite outgrowth and retraction was investigated in PC12 cells and SH-SY5Y human neuroblastoma cells. ODQ inhibited neurite outgrowth and triggered neurite retraction in the cells stimulated with ner...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21838
更新日期：2009-01-01 00:00:00
