Abstract：
:Granulocyte colony-stimulating factor (G-CSF) and its related mechanisms were investigated to assess the potential for this factor to exert neuroprotective effects against spinal cord injury in mice. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was injected into mice spinal cord hemisection models. Locomotor activity was assessed by using the Basso-Bettie-Bresnahan scale. Neurons isolated from spinal cords were cultured in vitro and used in a neuronal mechanical injury model. Three treatment groups were compared with this model, 1) G-CSF, 2) G-CSF + NSC348884 (a nucleophosmin 1-specific inhibitor), and 3) NSC348884. Immunofluorescence staining and Western blotting were performed to analyze the expression of G-CSF and nucleophosmin 1 (Npm1). TUNEL staining was performed to analyze apoptosis after G-CSF treatment. We found that the G-CSF receptor (G-CSFR) and Npm1 were expressed in neurons and that Npm1 expression was induced after G-CSF treatment. G-CSF inhibited neuronal apoptosis. NSC348884 induced p53-dependent cell apoptosis and partially blocked the neuroprotective activity of G-CSF on neurons in vitro. G-CSF promoted locomotor recovery and demonstrated neuroprotective effects in an acute spinal cord injury model. The mechanism of G-CSF's neuroprotection may be related in part to attenuating neuronal apoptosis by NPM1.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Guo Y,Liu S,Wang P,Zhang H,Wang F,Bing L,Gao J,Yang J,Hao Adoi
10.1002/jnr.23362subject
Has Abstractpub_date
2014-06-01 00:00:00pages
751-60issue
6eissn
0360-4012issn
1097-4547journal_volume
92pub_type
杂志文章abstract：:Despite the accumulating data on the molecular and cell biological characteristics of neural stem/progenitor cells, their electrophysiological properties are not well understood. In the present work, changes in the membrane properties and current profiles were investigated in the course of in vitro-induced neuron form...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21282
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abstract：:Histology, immunohistochemistry, and Western blotting were used to characterize the changes in morphology, distribution pattern, and marker protein expression of striatal interneurons in the transition zone of striatal injury induced by 3-NP. The 3-NP treatment in rats yielded movement, motor coordination, and cogniti...
journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490420316
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doi：10.1002/jnr.490290409
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journal_title：Journal of neuroscience research
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pub_type： 杂志文章
doi：10.1002/jnr.490090309
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pub_type： 杂志文章
doi：10.1002/jnr.490060602
更新日期：1981-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980601)52:5<538::AID-JNR
更新日期：1998-06-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490290304
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10567
更新日期：2003-04-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23677
更新日期：2016-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490220411
更新日期：1989-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490150312
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490370512
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
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journal_title：Journal of neuroscience research
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pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300117
更新日期：1991-09-01 00:00:00
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19971015)50:2<202::AID-JNR
更新日期：1997-10-15 00:00:00
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490170316
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