Abstract:
:Cultured purified oligodendroglia and astroglia exposed to heat stress (45 degrees C, 10 or 20 min) synthesized a 68-kDa heat-stress protein, which migrates on two-dimensional gel electrophoresis and reacts with a specific monoclonal antibody suggesting it is similar to a major 72-kDa heat-shock protein previously reported in other cell types. This protein was not detected in control glial cultures. Actinomycin D prevented synthesis of this protein demonstrating an absolute requirement for newly synthesized mRNA. The response was prolonged by increasing the period of heat stress from 10 to 20 min. In addition to the 68-kDa HSP protein, the incorporation of radioactivity into 70-, 89-, and 97-kDa proteins was also increased after heating, but in contrast to the 68 kDa protein these proteins appeared to be made in control glial cultures.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Nishimura RN,Dwyer BE,Welch W,Cole R,de Vellis J,Liotta Kdoi
10.1002/jnr.490200103subject
Has Abstractpub_date
1988-05-01 00:00:00pages
12-8issue
1eissn
0360-4012issn
1097-4547journal_volume
20pub_type
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