Abstract:
:Neurofibromatosis type 2 is a hereditary cancer syndrome characterized by the development of bilateral vestibular schwannomas. Underlying the disease are inactivating mutations of the NF2 tumor suppressor gene, located on chromosome 22, encoding a 595-amino-acid protein. The NF2 protein, also known as merlin or schwannomin, is reported to act as a membrane-cytoskeleton linking protein. This assumption is based on the homology of the NF2 protein to a group of band 4.1-related proteins, ezrin, radixin, and moesin. The cytoskeletal association of the NF2 protein has in part been confirmed by its ability to resist extraction from cells by nonionic detergents. We performed detergent extraction on COS cells transfected with NF2 cDNA constructs. The extracts were analyzed by Western blotting and immunofluorescent staining with monoclonal anti-NF2 antibodies. The results provide evidence for a high-affinity cytoskeleton attachment domain at amino acids 29-131 and a putative lower affinity domain between amino acids 321 and 470.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
den Bakker MA,Riegman PH,Suurmeijer AP,Vissers CJ,Sainio M,Carpen O,Zwarthoff ECdoi
10.1002/1097-4547(20001215)62:6<764::AID-JNR2>3.0.keywords:
subject
Has Abstractpub_date
2000-12-15 00:00:00pages
764-71issue
6eissn
0360-4012issn
1097-4547pii
10.1002/1097-4547(20001215)62:6<764::AID-JNR2>3.0.journal_volume
62pub_type
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