Abstract:
:A study was conducted on the adsorption of 14C-ATP to a surface film of a hydrophobic protein derived from synaptic membranes isolated from bovine cerebellum. The adsorption of ATP to the protein film followed a rate law based on diffusion and an energy barrier to adsorption, the rate law being generally applicable to the adsorption of ions to a charged interface. Studies were also carried out on the displacement of ATP from the film by the injection of other nucleotides, inorganic phosphates, and other anions. The kinetics conformed to a rate law based on diffusion and displacement. The most important factor in the displacement of ATP was the magnitude of charge of the anion, while steric factors were relatively minor. However, from a consideration of the energy barrier to adsorption it appeared that steric factors play a greater role in the adsorption of ATP. The results are discussed in relationship to the configurational aspects of the surface film as well as their possible significance in synaptic function.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Hoss W,Abood LGdoi
10.1002/jnr.490020109subject
Has Abstractpub_date
1976-01-01 00:00:00pages
71-81issue
1eissn
0360-4012issn
1097-4547journal_volume
2pub_type
杂志文章abstract::Relationships among different intensities of electroshock treatment (EST), EEG-recordable primary after discharge (PAD), and beta-adrenergic binding in various brain regions were investigated in adult rats. Different intensities of EST (1-2, 6-8, 20-30 mamp) were applied through ear-clip electrodes for 7 consecutive d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490070306
更新日期:1982-01-01 00:00:00
abstract::An increased level of myelin basic protein (MBP) degradation peptide 80-89, representative of myelin breakdown, is detected in myelinating foetal rat brain aggregate cultures supplemented with peritoneal macrophages at a time coinciding with the onset of myelination. During the period of myelination, the proportion of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10026
更新日期:2001-12-15 00:00:00
abstract::Cerebrospinal fluid was collected from the lateral ventricles and cisternal space from beagle dogs under light pentobarbital anesthesia. Homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglyco-sulfate (MOPEG-SO4) levels in the liquor taken from these two regions were evaluated,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010513
更新日期:1975-01-01 00:00:00
abstract::Bioassays of factors promoting nerve growth in embryonic ganglia are described. Chick embryo extract evokes fiber outgrowth in both sympathetic and ciliary ganglia explanted into a collagen gel. The response is not suppressed by antibodies directed against mouse nerve growth factor (NGF). Chick embryo extract also sup...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490080206
更新日期:1982-01-01 00:00:00
abstract::Chondroitinase ABC (Ch'ase ABC) is a bacterial lyase that degrades chondroitin sulfate (CS), dermatan sulfate, and hyaluronan glycosaminoglycans (GAGs). This enzyme has received significant attention as a potential therapy for promoting central nervous system and peripheral nervous system repair based on its degradati...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21199
更新日期:2007-04-01 00:00:00
abstract::An inflammatory response involving activated microglia in neuritic beta-amyloid plaques is found in Alzheimer's disease (AD) brain. Because HDL lipoproteins have been shown to carry the beta-amyloid peptide (Abeta) in plasma and CSF, we have investigated the influence of plasma high-density lipoprotein (HDL) and lipid...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-08-15 00:00:00
abstract::Production of hydrogen peroxide as a by-product of the breakdown of catecholamines by the enzyme monoamine oxidase (MAO) has been hypothesized to contribute to the increased proclivity of dopaminergic neurons for oxidative injury. We established clonal dopaminergic PC12 cell lines which have elevated MAO activity leve...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19961215)46:6<666::AID-JNR
更新日期:1996-12-15 00:00:00
abstract::The changes in ganglioside composition and metabolism of deafferentiated rat hippocampus were estimated after septal lesion. A significant decrease in total ganglioside concentration was found 7 days after the lesion. The reduced level of total gangliosides persisted at 17 and 25 days. Relative increase in the proport...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490210107
更新日期:1988-09-01 00:00:00
abstract::In order to study the voltage-dependent ion channels in microglia, and their possible modulation by pro-inflammatory substances like lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) we employed the patch-clamp technique on purified rat microglial cell subcultures grown for 1 - 5 days in control condition or a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420402
更新日期:1995-11-01 00:00:00
abstract::A novel approach was developed to raise a panel of monoclonal antibodies (mAb) against brain antigens using Xenopus oocytes as immunological vectors. Xenopus oocytes were injected to express proteins encoded by brain-derived mRNA extracted from rat cerebral cortex. A crude membrane preparation from mRNA-injected oocyt...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290109
更新日期:1991-05-01 00:00:00
abstract::Plexiform layer synaptic and photoreceptor cell components were investigated in retinas of Royal College of Surgeons (RCS) dystrophic rats transplanted with normal retinal pigment epithelial (RPE) cells by immunocytochemistry using previously characterized monoclonal antibodies. In retinas of normal adult rats and RPE...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490360408
更新日期:1993-11-01 00:00:00
abstract::An early and essential step in the formation of functional neuronal circuits is the establishment of cell polarity, a process involving the morphological and functional differentiation of the axon (axonogenesis). We now report that treatment of cultured embryonic hippocampal neurons with ethidium bromide (EtBr; an age...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990401)56:1<8::AID-JNR2>
更新日期:1999-04-01 00:00:00
abstract::The induction of an inflammatory response and release of cytokines such as TNF may be involved in the age-related etiology of Alzheimer disease (AD). In the brain, microglia have been shown to produce a wide variety of immune mediators, including the pro-inflammatory cytokine tumor necrosis factor (TNF). We hypothesiz...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1097
更新日期:2001-06-01 00:00:00
abstract::Alpha2-adrenergic receptors control norepinephrine (NE) release and tumor necrosis factor-alpha (TNF) production from neurons. TNF regulates NE release, depending on alpha2-adrenergic receptor functioning. The relationship between TNF production in the brain and alpha2-adrenergic receptor activation could have profoun...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20407
更新日期:2005-03-15 00:00:00
abstract::Cross-linking of alpha-synuclein and Lewy body formation have been implicated in the dopaminergic neuronal cell death observed in Parkinson's disease (PD); the mechanisms responsible, however, are not clear. Reactive oxygen species and advanced glycation end products (AGEs) have been found in the intracellular, alpha-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21644
更新日期:2008-07-01 00:00:00
abstract::The postmortem stability of brain RNA was measured in rat and human samples for up to 48 hr. Whole rat brains, cooled at a rate approximating that of human brains awaiting autopsy, were collected at intervals from 0 to 48 hr after death and frozen. These samples were compared with freshly obtained, unfrozen rat brains...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490160123
更新日期:1986-01-01 00:00:00
abstract::Fetal low brain oxygenation may be an outcome of maternal complications during pregnancy and is associated with increased risk of cerebral palsy and periventricular leukomalacia in newborns. One treatment used for prevention of fetal brain damage is maternal treatment with MgSO(4). Although this treatment is indicated...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20269
更新日期:2004-11-01 00:00:00
abstract::Ischemia elicits a variety of stress responses in neuronal cells, which result in cell death. wld(S) Mice bear a mutation that significantly delays Wallerian degeneration. This mutation also protects all neuronal cells against other types of stresses resulting in cell death, including ischemia. To clarify the types of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22792
更新日期:2012-03-01 00:00:00
abstract::Primary fibroblasts initiated from skin biopsies of Wistar rats were transfected with a plasmid that encodes the human growth hormone and the neomycin resistance genes. Cell clones selected for G418 resistance and expressing high levels of human growth hormone were propagated in vitro and subsequently transplanted int...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290304
更新日期:1991-07-01 00:00:00
abstract::The mechanisms underlying brain seizure tolerance, a phenomenon in which brief periods of seizures protect brain against the lethal effects of subsequent sustained seizures, are poorly understood. Because brain seizure tolerance and brain ischemia tolerance likely share certain common mechanisms, the recent evidence t...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20566
更新日期:2005-08-15 00:00:00
abstract::The possibility that neuron-astrocyte communication may be responsible for glutamate (Glu)-stimulated cGMP formation even in relatively homogeneous primary cultures of mouse cerebellar granule cells (7 days in vitro) was investigated. Pharmacological analysis using selective excitatory amino acid (EAA) receptor antago...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960715)45:2<129::AID-JNR
更新日期:1996-07-15 00:00:00
abstract::Multiple sclerosis (MS) is an immune-mediated demyelinating disorder of unknown etiology. Both genetic-susceptibility and environment exposures, including vitamin D deficiency, Epstein-Barr viral and Herpesvirus (HHV-6) infections are strongly implicated in the activation of T cells and MS-pathogenesis. Despite precis...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24322
更新日期:2018-12-01 00:00:00
abstract::We sought to establish a mouse model of subcortical ischemic vascular dementia (SIVD) that develops predominant white matter (WM) injury and cognitive dysfunction induced by chronic cerebral hypoperfusion. Adult C57Bl/6 male (n = 48) mice were subjected to bilateral common carotid artery stenosis with external microco...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21925
更新日期:2009-04-01 00:00:00
abstract::Because there is a strong correlation between tuberous sclerosis and autism, we used a tuberous sclerosis model (Eker rat) to test the hypothesis that these animals would have an altered regional cerebral O2 consumption that might be associated with autism. We also examined whether the altered cerebral O2 consumption ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21378
更新日期:2007-08-15 00:00:00
abstract::In the rat brain, a heteromeric association between adenosine A(1) and purinergic P2Y(1) receptors has been demonstrated. It is suggested that this association plays an important role in the control of purine-mediated responses during pathophysiological conditions. Recently, we have demonstrated that these receptors c...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21727
更新日期:2008-10-01 00:00:00
abstract::We report a study of the characterization of voltage-activated potassium (K+) currents in retinal ON bipolar cells in zebrafish. At single-channels levels, the open probability of the K+ channels increased when the membrane potential was increased. The maximal open proportion was 0.76+/-0.05 under our testing conditio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20637
更新日期:2005-11-01 00:00:00
abstract::Four new monoclonal antibodies to the extracellular domain of the nerve growth factor receptor (NGFR) have been evaluated for their specificity to NGFR and their utility in localizing NGFR in human brain. All four antibodies, as well as Me20.4, show similar cellular localization and patterns of immunoreactivity in bas...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270426
更新日期:1990-12-01 00:00:00
abstract::Persephin (PSP) is the most recently discovered member of the GDNF family of neurotrophic factors. We have used an RT-PCR approach to start addressing the putative functional significance of PSP by determining sites of its synthesis in the neonatal rat brain. Generally, two transcripts were found. Sequence analysis of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980815)53:4<494::AID-JNR
更新日期:1998-08-15 00:00:00
abstract::Neuroepithelial b2T cells were derived from the hindbrain and the spinal cord of mouse transgenic embryos, which expressed SV40 T antigen under the control of a Hoxb2 enhancer. Strikingly, b2T cell lines of either origin exhibit a very similar gene expression pattern, including markers of the hindbrain and the spinal ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10716
更新日期:2003-09-15 00:00:00
abstract::In a nominally calcium-free medium, a toxic phospholipase A2, paradoxin, PDX (1-200nM) was able to significantly decrease glutamate uptake by rat hippocampal mini-slices. Under the same experimental conditions, PDX could also inhibit the reuptake of choline and dopamine, suggesting a nonselective action. Furthermore, ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980201)51:3<349::AID-JNR
更新日期:1998-02-01 00:00:00