Abstract:
:Recent structural studies of epidermal growth factor receptor (EGFR) family extracellular regions have identified an unexpected mechanism for ligand-induced receptor dimerization that has important implications for activation and inhibition of these receptors. Here we describe the 2.8 angstroms resolution X-ray crystal structure of the antigen binding (Fab) fragment from cetuximab (Erbitux), an inhibitory anti-EGFR antibody, in complex with the soluble extracellular region of EGFR (sEGFR). The sEGFR is in the characteristic "autoinhibited" or "tethered" inactive configuration. Cetuximab interacts exclusively with domain III of sEGFR, partially occluding the ligand binding region on this domain and sterically preventing the receptor from adopting the extended conformation required for dimerization. We suggest that both these effects contribute to potent inhibition of EGFR activation.
journal_name
Cancer Celljournal_title
Cancer cellauthors
Li S,Schmitz KR,Jeffrey PD,Wiltzius JJ,Kussie P,Ferguson KMdoi
10.1016/j.ccr.2005.03.003keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
301-11issue
4eissn
1535-6108issn
1878-3686pii
S1535-6108(05)00090-5journal_volume
7pub_type
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