Localization and characterization of glutathione-s-transferase isozymes alpha, mu, and pi within the mouse vomeronasal organ.

Abstract:

:The nasal cavity of vertebrates contains a variety of xenobiotic metabolizing enzymes that possess a broad range of substrate specificity ranging from metabolism of drugs, carcinogens, and steroid hormones, to dietary components and environmental pollutants. This investigation sought to localize the cellular expression and distribution of glutathione-s-transferase (GST) alpha, mu, and pi detoxifying enzymes, and to study GST activity toward different substrates in the mouse vomeronasal organ (VNO). Immunohistochemistry was used to identify GST alpha, mu and pi in the non-sensory and sensory layer of the VNO. Western blot analysis of cytosolic proteins revealed a qualitatively higher enzyme expression of GST alpha and mu in the main olfactory tissue (OE) in comparison to VNO tissue, whereas the GST pi isozyme was equally expressed in both. Total GST metabolism of 1-chloro-2, 4-dinitrobenzene (CDNB) revealed a higher activity level in the OE when compared to the VNO. In contrast, thin-layer chromatographic analysis of GST conjugation of the odorant, trans-2-hexenal (t-hex) (10 mM) showed more conjugate formed per unit protein in the VNO than the OE. The analysis of GST expression and enzyme activity within the VNO parallels the reported localization of phase I metabolizing enzymes and suggests that GST isozymes play independent roles that characterize multiple processes within VNO chemosensitivity.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Green N,Weech M,Walters E

doi

10.1016/j.neulet.2004.11.037

keywords:

subject

Has Abstract

pub_date

2005-03-03 00:00:00

pages

198-202

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(04)01407-7

journal_volume

375

pub_type

杂志文章
  • Circadian rhythm of cortical acetylcholine release as measured by in vivo microdialysis in freely moving rats.

    abstract::Circadian changes in cortical acetylcholine (ACh) release were examined in freely moving rats using long-term in vivo microdialysis and high performance liquid chromatography with electrochemical detection. A microdialysis probe was implanted into the frontal cortex and a dialysate was collected every 30 min for two o...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(91)90316-l

    authors: Kametani H,Kawamura H

    更新日期:1991-11-11 00:00:00

  • Lesions to Schaffer collaterals prevent ischemic death of CA1 pyramidal cells.

    abstract::The contribution of excitatory inputs to CA1 pyramidal cell death after ischemia was examined using rats with unilateral destruction of CA3 pyramidal cells. Intracerebroventricular injection of L-alpha-kainic acid (KA) was performed before the induction of transient forebrain ischemia. Five days after ischemic insult,...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(86)90136-9

    authors: Onodera H,Sato G,Kogure K

    更新日期:1986-07-24 00:00:00

  • NADPH-diaphorase reactivity is reduced in the molecular layer but increased in the granular layer of primate cerebellum after prolonged anaesthesia.

    abstract::To test if nitric oxide (NO) is involved in modulation of neuronal activity after global changes of brain function, nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reactivity in the cerebellar cortex was compared in monkeys maintained under anaesthesia for 24-48 h with others only anaesthetised for pe...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/s0304-3940(97)00497-7

    authors: Yan XX,Garey LJ

    更新日期:1997-07-18 00:00:00

  • Structured movement representations of a phantom limb associated with phantom limb pain.

    abstract::The relation between phantom limb pain (PLP) and the movement representation of a phantom limb remains controversial in several areas of neurorehabilitation, although there are a few studies in which the representation of phantom limb movement was precisely evaluated. We evaluated the structured movement representatio...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2015.08.009

    authors: Osumi M,Sumitani M,Wake N,Sano Y,Ichinose A,Kumagaya S,Kuniyoshi Y,Morioka S

    更新日期:2015-09-25 00:00:00

  • Diversity in localisation of nitric oxide synthase antigen and NADPH-diaphorase histochemical staining in sacral somatic motor neurones of the cat.

    abstract::Nitric oxide synthase (NOS) immunoreactivity occurred in about 60% of ventromedial, ventrolateral, and sphincteric motoneurones in cat sacral spinal cord. Proportions of sacral motoneurones histochemically stained for NADPH-diaphorase, were similar, and equivalent in size to those immunoreactive for NOS, suggesting co...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(95)11831-g

    authors: Pullen AH,Humphreys P

    更新日期:1995-08-18 00:00:00

  • Histochemical demonstration of separate populations of somatostatin and cholinergic neurons in the rat striatum.

    abstract::Striatal neurons containing acetylcholine and somatostatin were examined using a pharmacohistochemical procedure for acetylcholinesterase and NADPH-diaphorase histochemistry respectively. The use of these two histochemical procedures allowed both cholinergic and somatostatin cells to be visualized simultaneously in si...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(83)90536-0

    authors: Vincent SR,Staines WA,Fibiger HC

    更新日期:1983-02-21 00:00:00

  • Dilation of the inferior colliculus and hypersensitivity to sound in Wnt1-cre and Wnt1-GAL4 double-transgenic mice.

    abstract::The processing of sound information is mediated by the cochlea and the central auditory system. Among the central auditory system, the inferior colliculus (IC) has leading roles in the acoustic processing. In a previous study, we demonstrated psychiatric disorder-related behavioral abnormalities in a genetically modif...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2014.02.061

    authors: Nakajima M,Nishikawa C,Miyasaka Y,Kikkawa Y,Mori H,Tsuruta M,Okuyama S,Furukawa Y

    更新日期:2014-04-30 00:00:00

  • Anterior cingulate GABA levels predict whole-brain cerebral blood flow.

    abstract::This study examined the relationship of γ-aminobutyric acid (GABA) and glutamate levels from the anterior and posterior cingulates (AC and PC) with cerebral blood flow (CBF) at rest. (1)H magnetic resonance spectroscopy measurements in the AC and PC and pseudo-continuous arterial spin labeling data were acquired from ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2013.12.062

    authors: Krause BW,Wijtenburg SA,Holcomb HH,Kochunov P,Wang DJ,Hong LE,Rowland LM

    更新日期:2014-02-21 00:00:00

  • Release of proteins during long-term potentiation in the hippocampus of the anaesthetized rat.

    abstract::Using a push-pull device, the release of endogenous proteins in the extracellular space was investigated in the CA1 region of the hippocampus of anaesthetized rats. With low-frequency stimulation of the Schaffer collaterals, there was a relatively stable release of 5 proteins (64, 54, 48, 45 and 16 kDa). A train of hi...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(88)90698-2

    authors: Charriaut-Marlangue C,Aniksztejn L,Roisin MP,Ben-Ari Y

    更新日期:1988-09-12 00:00:00

  • Lack of vascular endothelial growth factor receptor-2/Flk1 signaling does not affect substantia nigra development.

    abstract::Oxygen tension is critical for proliferation of human and murine midbrain-derived neural precursor cells (mNPCs). Lack of hypoxia-inducible factor-1α (HIF1α) impairs midbrain dopaminergic neurogenesis which could be rescued by vascular endothelial growth factor (VEGF) via VEGFR-2 signaling. Here, we conditionally inac...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2013.08.031

    authors: Hermann A,Loewenbrück KF,Boldt Ä,Fiedler C,Maisel M,Kalucka J,Schwarz J,Breier G,Storch A

    更新日期:2013-10-11 00:00:00

  • Perinatal opiate treatment delays growth of cortical dendrites.

    abstract::Basilar dendritic arborizations of layer II-III pyramidal neurons in primary somatosensory cortex of 5-day-old male rats were reconstructed following perinatal morphine, morphine/naltrexone, or saline vehicle administration. Morphine treatment was observed to reduce total dendritic length. This effect was limited to h...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(90)90444-e

    authors: Ricalde AA,Hammer RP Jr

    更新日期:1990-07-31 00:00:00

  • Gene expression and association analysis of the epithelial membrane protein 1 gene in major depressive disorder in the Japanese population.

    abstract::The epithelial membrane protein 1 (EMP1) plays a role in neuronal differentiation and neurite outgrowth, which are involved in the pathogenesis of major depressive disorder (MDD). We sought to determine whether the EMP1 gene is implicated in MDD. We determined the mRNA expression levels of the EMP1 gene in peripheral-...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2010.12.001

    authors: Nakataki M,Iga J,Numata S,Yoshimoto E,Kodera K,Watanabe SY,Song H,Ueno S,Ohmori T

    更新日期:2011-02-04 00:00:00

  • Traumatic brain injury stimulates hippocampal catechol-O-methyl transferase expression in microglia.

    abstract::Outcome following traumatic brain injury (TBI) is in large part determined by the combined action of multiple processes. In order to better understand the response of the central nervous system to injury, we utilized an antibody array to simultaneously screen 507 proteins for altered expression in the injured hippocam...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2006.11.060

    authors: Redell JB,Dash PK

    更新日期:2007-02-08 00:00:00

  • Induction of Fos-like immunoreactivity in rat oxytocin neurons following insulin injections.

    abstract::Double immunostaining for oxytocin (OT) and Fos was used to study the oxytocinergic system of the rat hypothalamic paraventricular nucleus (PVN) following intraperitoneal insulin injections. The expression of c-fos in the PVN appeared about 3 h after insulin treatment and was very high after 5 h while no labelling was...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(94)90304-2

    authors: Griffond B,Deray A,Bahjaoui-Bouhaddi M,Colard C,Bugnon C,Fellmann D

    更新日期:1994-08-29 00:00:00

  • Coexistence of galanin and substance P in the mouse nasal mucosa, including the vomeronasal organ.

    abstract::Immunohistochemical fluorescent double labeling revealed the coexistence of galanin and substance P in nerve fibers in the mouse nasal mucosa. At the base of and in the epithelium, all galanin fibers also contained substance P, but around the blood vessels and glands, most of them did not. Since substance P fibers in ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(94)90148-1

    authors: Matsuda H,Tsukuda M,Kadota T,Kusunoki T,Kishida R

    更新日期:1994-05-23 00:00:00

  • Negative symptoms of schizophrenia could explain discrepant data on the association between the 5-HT2A receptor gene and response to antipsychotics.

    abstract::Pharmacogenetic studies assessing the role of 5-HT(2A) receptor gene in antipsychotic efficacy yielded conflicting data. Phenotypical heterogeneity of schizophrenia might explain such discrepancies. For example, negative symptoms are known to reflect severity of illness and to restrain therapeutic response. On this ba...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2004.11.070

    authors: Hamdani N,Bonnière M,Adès J,Hamon M,Boni C,Gorwood P

    更新日期:2005-03-22 00:00:00

  • Metabolomic analysis to detect urinary molecular changes associated with bipolar depression.

    abstract::Bipolar disorder (BD) is a debilitating mental disorder with complex clinical manifestations and low diagnostic accuracy. Depressive episodes are most common in the course of BD with high comorbidity and suicide rates, which present greater clinical challenges than mania and hypomania episodes. However, there are no o...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2020.135515

    authors: Ren Y,Chen ZZ,Sun XL,Duan HJ,Tian JS,Wang JY,Yang H

    更新日期:2021-01-18 00:00:00

  • Thyrotropin-releasing hormone enhances event-related brain potentials and growth hormone release in man.

    abstract::Changes in contingent negative variation (CNV), a brain-evoked potential related in arousal, as well as in serum triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), prolactin (PRL), cortisol and growth hormone (GH) levels were recorded in 12 male volunteers receiving either thyrotropin-releasing ...

    journal_title:Neuroscience letters

    pub_type: 临床试验,杂志文章

    doi:10.1016/0304-3940(88)90107-3

    authors: Strollo F,Amabile G,Fattapposta F,Strollo G,More M,Riondino G

    更新日期:1988-11-11 00:00:00

  • Alterations in the expression of PSA-NCAM and synaptic proteins in the dorsolateral prefrontal cortex of psychiatric disorder patients.

    abstract::Alterations in the structure and physiology of the prefrontal cortex (PFC) have been found in different psychiatric disorders and some of them involve inhibitory networks, especially in schizophrenia and major depression. Changes in the structure of these networks may be mediated by the polysialylated neural cell adhe...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2012.09.032

    authors: Gilabert-Juan J,Varea E,Guirado R,Blasco-Ibáñez JM,Crespo C,Nácher J

    更新日期:2012-11-14 00:00:00

  • Axotomy affects calcium-sensitive potassium conductance in sympathetic neurones.

    abstract::Following peripheral nerve section, the cell body and proximal axon undergo various physiological and morphological changes which may be associated with regrowth. We found that axotomy reduced the amplitude and duration of the afterhyperpolarization (AHP) of the action potential (AP) of bullfrog sympathetic neurones. ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(86)90391-5

    authors: Kelly ME,Gordon T,Shapiro J,Smith PA

    更新日期:1986-06-18 00:00:00

  • Statistical learning is lasting and consistent over time.

    abstract::Implicit detection of statistical regularities is thought to be a ubiquitous facet of cognition; yet, we know little about statistical learning (SL) over time. A recent study showed that visual SL can be observed at 24 h post stimulus (Kim et al., 2009 [14]). Here we sought to obtain a finer-grained picture of visual ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2012.04.045

    authors: Arciuli J,Simpson IC

    更新日期:2012-05-31 00:00:00

  • The negative influence of endogenous opioid receptor activity on the differentiation of the rat pheochromocytoma PC12 cells induced by nerve growth factor.

    abstract::We determined the effect of naloxone and morphine on the differentiation of pheochromocytoma cell, the PC12 cells, induced by nerve growth factor (NGF). PC12 cells were grown in medium containing NGF with or without the addition of naloxone or morphine for up to 10-day treatments. NGF-induced morphological differentia...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/s0304-3940(98)00528-x

    authors: Yeh GC,Hsieh TH,Chang SF

    更新日期:1998-08-07 00:00:00

  • MDM2 antagonism by nutlin-3 induces death in human medulloblastoma cells.

    abstract::A critical component of the cellular stress response, the p53 tumor suppressor protein must be functional for many cancer therapies to be effective. Adjuvant therapies that augment p53 function are predicted to sensitize tumor cells to cancer therapies that rely upon p53 for their efficacy. Of those strategies current...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2012.02.022

    authors: Ghassemifar S,Mendrysa SM

    更新日期:2012-03-28 00:00:00

  • GSM radiation triggers seizures and increases cerebral c-Fos positivity in rats pretreated with subconvulsive doses of picrotoxin.

    abstract::This study investigated the effects of mobile-phone-type radiation on the cerebral activity of seizure-prone animals. When rats transformed into an experimental model of seizure-proneness by acute subconvulsive doses of picrotoxin were exposed to 2 h GSM-modulated 900 MHz radiation at an intensity similar to that emit...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2005.12.082

    authors: López-Martín E,Relova-Quinteiro JL,Gallego-Gómez R,Peleteiro-Fernández M,Jorge-Barreiro FJ,Ares-Pena FJ

    更新日期:2006-05-01 00:00:00

  • Thyrotropin-releasing hormone (TRH) degradation in NIH:N mouse cerebral cortex and spinal cord.

    abstract::Thyrotropin-releasing hormone (TRH) metabolism in NIH:N mouse cerebral cortex and spinal cord was studied with high pressure liquid chromatography. Degradation of added TRH was significantly (P less than 0.001) slower in the spinal cord (2.19 +/- 0.46%/min) than in the cerebral cortex (7.54 +/- 0.31%/min). Bacitracin,...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(86)90560-4

    authors: Schwartz TM,Turner JG,Brooks BR

    更新日期:1986-11-11 00:00:00

  • Characterization of epithelial V-like antigen in human choroid plexus epithelial cells: potential role in CNS immune surveillance.

    abstract::Prior work demonstrated that immune surveillance of the brain occurs primarily through the blood-cerebrospinal (CSF) fluid barrier rather than the blood-brain barrier endothelium. Recently, we identified epithelial V-like antigen (EVA), an immunoglobulin-like adhesion molecule, as a regulator of blood-CSF barrier inte...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2011.03.051

    authors: Wojcik E,Carrithers LM,Carrithers MD

    更新日期:2011-05-16 00:00:00

  • Deficits of [3H]D-aspartate binding to glutamate uptake sites in striatal and accumbens tissue in patients with schizophrenia.

    abstract::The hypothesis involving glutamate in the neuropathology of schizophrenia has attracted great interest. Several studies report dysfunctions in glutamatergic systems, including alterations in kainate and N-methyl-D-aspartate (NMDA) receptors in various areas, as well as changes in the number of glutamate uptake sites. ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/s0304-3940(97)00563-6

    authors: Aparicio-Legarza MI,Cutts AJ,Davis B,Reynolds GP

    更新日期:1997-08-22 00:00:00

  • Basic fibroblast growth factor enhances cell proliferation in the dentate gyrus of neonatal rats following hypoxic-ischemic brain damage.

    abstract:BACKGROUND:Perinatal hypoxic-ischemic insult is considered a major contributor to child mortality and morbidity and leads to neurological deficits in newborn infants. There has been a lack of promising neurotherapeutic interventions for hypoxic-ischemic brain damage (HIBD) for clinical application in infants. The prese...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2018.01.046

    authors: Zhu H,Qiao L,Sun Y,Yin L,Huang L,Jiang L,Li J

    更新日期:2018-04-23 00:00:00

  • Early maternal deprivation induces alterations in brain-derived neurotrophic factor expression in the developing rat hippocampus.

    abstract::The effects of maternal deprivation (MD) during early postnatal life on the brain-derived neurotrophic factor (BDNF) level were investigated in the present study. Wistar rats were assigned to either maternal deprivation or mother-reared control (MRC) groups. MD manipulation was achieved by separating rat pups from the...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/j.neulet.2004.09.012

    authors: Kuma H,Miki T,Matsumoto Y,Gu H,Li HP,Kusaka T,Satriotomo I,Okamoto H,Yokoyama T,Bedi KS,Onishi S,Suwaki H,Takeuchi Y

    更新日期:2004-11-30 00:00:00

  • Pharmacological investigation on the role of dopamine in the rat locus coeruleus.

    abstract::The role of dopamine (DA) in the rat locus coeruleus (LC) was investigated by determining the levels of 3,4-dihydroxyphenylacetic acid (DOPAC), DA and noradrenaline (NA) in the LC after pharmacological treatments by pargyline, haloperidol, 6-hydroxydopamine (6-OHDA) and desmethylimipramine (DMI). The DA, DOPAC and NA ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章

    doi:10.1016/0304-3940(82)90416-5

    authors: Milon H,McRae-Degueurce A

    更新日期:1982-06-30 00:00:00