Preparation of human hepatocellular carcinoma-targeted liposome microbubbles and their immunological properties.

Abstract:

AIM:To prepare the human hepatocellular carcinoma-(HCC)-targeted liposome microbubbles and to investigate their immunological properties. METHODS:Human hepatocarcinoma specific monoclonal antibody HAb18 was attached to the surface of home-made liposome microbubbles by static attraction to prepare the targeted liposome microbubbles. The combination of HAb18 with liposome microbubbles was confirmed by the slide agglutination test and immunofluorescent assay. Their immunological activity was measured by ELISA. Rosette formation test, rosette formation blocking test and immun-ofluorescent assay were used to identify the specific binding of targeted liposome microbubbles to SMMC-7721 hepatoma cells, and cytotoxicity assay was used to detect their effect on human hepatocytes. RESULTS:The targeted liposome microbubbles were positive in the slide agglutination test and immunofluorescent assay. ELISA indicated that the immunological activity of HAb18 on the liposome microbubbles was similar to that of free HAb18. SMMC-7721 cells were surrounded by the targeting liposome microbubbles to form rosettes, while the control SGC-7901 gastric cancer cells were not. Proliferation of SMMC-7721 cells and normal human hepatocytes was not influenced by the targeted liposome microbubbles. CONCLUSION:The targeted liposome microbubbles with a high specific biological activity have been successfully prepared, which specifically bind to human hepatocarcinoma cells, and are non-cytotoxic to hepatocytes. These results indicate that the liposome microbubbles can be used as a HCC-targeted ultrasound contrast agent that may enhance ultrasound images and thus improve the diagnosis of HCC, especially at the early stage.

journal_name

World J Gastroenterol

authors

Bian AN,Gao YH,Tan KB,Liu P,Zeng GJ,Zhang X,Liu Z

doi

10.3748/wjg.v10.i23.3424

keywords:

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

3424-7

issue

23

eissn

1007-9327

issn

2219-2840

journal_volume

10

pub_type

杂志文章
  • Evaluation of contrast-enhanced computed tomographic colonography in detection of local recurrent colorectal cancer.

    abstract:AIM:To evaluate the diagnostic accuracy, sensitivity, specificity of contrast-enhanced computed tomographic colonography in detecting local recurrence of colorectal cancer. METHODS:From January 2000 to December 2004, 434 patients after potentially curative resection for invasive colorectal cancer were followed up for ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i1.123

    authors: You YT,Chang Chien CR,Wang JY,Ng KK,Chen JS,Tang R,Chiang JM,Yeh CY,Hsieh PS

    更新日期:2006-01-07 00:00:00

  • Mesenteric inflammatory myofibroblastic tumors in an elder patient with early recurrence: a case report.

    abstract::Inflammatory myofibroblastic tumor (IMT) of the alimentary tract often occurs in children or young adults, but may occur at any age. Symptoms are nonspecific and depend on the location of the tumor. The most often involved sites are small bowel mesentery especially the distal ileum, mesotransverse colon, or great omen...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v13.i26.3645

    authors: Chen SS,Liu SI,Mok KT,Wang BW,Yeh MH,Chen YC,Chen IS

    更新日期:2007-07-14 00:00:00

  • EUS-guided drainage is more successful in pancreatic pseudocysts compared with abscesses.

    abstract:AIM:To compare the results for endoscopic ultrasound (EUS)-guided drainage of clear fluid pancreatic pseudocysts with the results for abscess drainage. METHODS:All patients referred for endoscopic drainage of a fluid collection were prospectively included. The outcome was recorded. RESULTS:Altogether 26 pseudocysts o...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v17.i4.499

    authors: Sadik R,Kalaitzakis E,Thune A,Hansen J,Jönson C

    更新日期:2011-01-28 00:00:00

  • Efficacy of docetaxel combined with oxaliplatin and fluorouracil against stage III/IV gastric cancer.

    abstract:AIM:To investigate the clinical efficacy and toxic effects of neoadjuvant chemotherapy using docetaxel combined with oxaliplatin and fluorouracil for treating stage III/IV gastric cancer. METHODS:A total of 53 stage III/IV gastric cancer patients were enrolled into the study and treated with neoadjuvant chemotherapy. ...

    journal_title:World journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3748/wjg.v20.i48.18413

    authors: Yu YJ,Sun WJ,Lu MD,Wang FH,Qi DS,Zhang Y,Li PH,Huang H,You T,Zheng ZQ

    更新日期:2014-12-28 00:00:00

  • Case of familial hyperlipoproteinemia type III hypertriglyceridemia induced acute pancreatitis: Role for outpatient apheresis maintenance therapy.

    abstract::Hypertriglyceridemic pancreatitis (HTGP) accounts for up to 10% of acute pancreatitis presentations in non-pregnant individuals and is the third most common cause of acute pancreatitis after alcohol and gallstones. There are a number of retrospective studies and case reports that have suggested a role for apheresis an...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v23.i40.7332

    authors: Saleh MA,Mansoor E,Cooper GS

    更新日期:2017-10-28 00:00:00

  • Liver-directed therapies for liver metastases from neuroendocrine neoplasms: Can laser ablation play any role?

    abstract::Aggressive cytoreduction can prolong survival in patients with unresectable liver metastases (LM) from neuroendocrine neoplasms (NEN), and minimally invasive, liver-directed therapies are gaining increasing interest. Catheter-based treatments are used in disseminated disease, whereas ablation techniques are usually in...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v26.i23.3118

    authors: Sartori S,Bianchi L,Di Vece F,Tombesi P

    更新日期:2020-06-21 00:00:00

  • Alcohol-induced steatosis in liver cells.

    abstract::Alcohol-induced fatty liver (steatosis) was believed to result from excessive generation of reducing equivalents from ethanol metabolism, thereby enhancing fat accumulation. Recent findings have revealed a more complex picture in which ethanol oxidation is still required, but specific transcription as well as humoral ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v13.i37.4974

    authors: Donohue TM Jr

    更新日期:2007-10-07 00:00:00

  • Evidence of human papilloma virus infection and its epidemiology in esophageal squamous cell carcinoma.

    abstract:AIM:To look for the evidence of human papilloma virus (HPV) infection in esophageal squamous cell carcinomas (ESCC) and to investigate the potential role and epidemiology of HPV infection in the pathogenesis of esophageal carcinomas in Henan emigrants. METHODS:Papilloma virus(PV) and HPV were determined by Ultrasensiv...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i9.1352

    authors: Yao PF,Li GC,Li J,Xia HS,Yang XL,Huang HY,Fu YG,Wang RQ,Wang XY,Sha JW

    更新日期:2006-03-07 00:00:00

  • Study of circumferential resection margin in patients with middle and lower rectal carcinoma.

    abstract:AIM:To clarify the relationship between circumferential resection margin status and local and distant recurrence as well as survival of patients with middle and lower rectal carcinoma. The relationship between circumferential resection margin status and clinicopathologic characteristics of middle and lower rectal carci...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v13.i24.3380

    authors: Wu ZY,Wan J,Li JH,Zhao G,Peng L,Yao Y,Du JL,Liu QF,Wang ZD,Huang ZM,Lin HH

    更新日期:2007-06-28 00:00:00

  • Relationship between antral distension and postprandial symptoms in functional dyspepsia.

    abstract:AIM:To investigate in patients with functional dyspepsia (FD) after an every-day meal whether (1) gastrointestinal (GI) and extra-GI symptoms had any relation with the degree of antral volume, (2) the onset of postprandial symptoms was associated with, and may predict, delayed gastric emptying. METHODS:In 94 symptomat...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i43.6982

    authors: Pallotta N,Pezzotti P,Corazziari E

    更新日期:2006-11-21 00:00:00

  • Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay.

    abstract:AIM:To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS:A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric canc...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i43.7029

    authors: Yang KC,Chu A,Liao CS,Lin YM,Wang GM

    更新日期:2006-11-21 00:00:00

  • Experimental study on mechanism and protection of stress ulcer produced by explosive noise.

    abstract::AIM:To establish an experimental model of stress ulcer produced by explosive noise, and to probe into its mechanism and protection.METHODS:The country standard Wistar white rats were randomly divided into control group(n =8), which were neither stimulated nor protected, and stimulating group (divided into subgroups A,...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v4.i6.519

    authors: Liu GS,Huang YX,Li SW,Pan BR,Wang X,Sun DY,Wang QL

    更新日期:1998-12-01 00:00:00

  • Effect of NDC80 in human hepatocellular carcinoma.

    abstract:AIM:To investigate the role of nuclear division cycle (NDC)80 in human hepatocellular carcinogenesis. METHODS:NDC80 gene expression was analyzed by real-time reverse transcription polymerase chain reaction in 47 paired hepatocellular carcinoma (HCC) and adjacent tissues. The HCC cell line SMMC-7721 was transfected wit...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i20.3675

    authors: Ju LL,Chen L,Li JH,Wang YF,Lu RJ,Bian ZL,Shao JG

    更新日期:2017-05-28 00:00:00

  • Prognosis following transcatheter arterial embolization for 121 patients with unresectable hepatocellular carcinoma with or without a history of treatment.

    abstract:AIM:To retrospectively evaluate the prognosis of patients with hepatocellular carcinoma (HCC) with or without a history of therapy for HCC following transcatheter arterial embolization (TAE). METHODS:One hundred and twenty-one patients with HCC treated with TAE from 1992 to 2004 in our hospital were enrolled in this s...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i13.2075

    authors: Hiraoka A,Kumagi T,Hirooka M,Uehara T,Kurose K,Iuchi H,Hiasa Y,Matsuura B,Michitaka K,Kumano S,Tanaka H,Yamashita Y,Horiike N,Mochizuki T,Onji M

    更新日期:2006-04-07 00:00:00

  • Resection of the uncinate process of the pancreas due to a ganglioneuroma.

    abstract::A 33-year-old woman who presented with epigastric discomfort and diarrhea underwent an abdominal ultrasound (US). This investigation and subsequent contrast-enhanced computed tomography, magnetic resonance imaging and endoscopic US with fine needle aspiration (FNA) revealed a 40 mm well-circumscribed mass in the uncin...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.15.4334

    authors: Poves I,Burdío F,Iglesias M,Martínez-Serrano Mde L,Aguilar G,Grande L

    更新日期:2009-09-14 00:00:00

  • Complete remission of advanced hepatocellular carcinoma by radiofrequency ablation after sorafenib therapy.

    abstract::Sorafenib, a potent multikinase inhibitor, lead to a significant improvement in progression free survival and overall survival in patients with advanced hepatocellular carcinoma (HCC). Though sorafenib has proven its efficacy in advanced stage HCC, there are limited reports on the role of sorafenib allowing for curati...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v21.i8.2568

    authors: Park JG,Park SY,Lee HW

    更新日期:2015-02-28 00:00:00

  • Predictive factors for lymph node metastasis in early gastric cancer.

    abstract:AIM:To analyze the predictive factors for lymph node metastasis (LNM) in early gastric cancer (EGC). METHODS:Data from patients surgically treated for gastric cancers between January 1994 and December 2007 were retrospectively collected. Clinicopathological factors were analyzed to identify predictive factors for LNM....

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v16.i41.5252

    authors: Sung CM,Hsu CM,Hsu JT,Yeh TS,Lin CJ,Chen TC,Su MY,Chiu CT

    更新日期:2010-11-07 00:00:00

  • Differences in characteristics of colorectal neoplasm between young and elderly Thais.

    abstract:AIM:To analyze the differences of clinical characteristics of colorectal neoplasm including polyps between the elderly and young Thai patients. METHODS:Colonoscopy database from December 2000 to October 2004 was retrospectively analyzed. There were 1822 eligible patients who underwent colonoscopy (with a mean age of 5...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i47.7684

    authors: Rerknimitr R,Ratanapanich W,Kongkam P,Kullavanijaya P

    更新日期:2006-12-21 00:00:00

  • Vitamin D improves viral response in hepatitis C genotype 2-3 naïve patients.

    abstract:AIM:To examine whether vitamin D improved viral response and predicted treatment outcome in patients with hepatitis C virus (HCV) genotype 2-3. METHODS:Fifty patients with chronic HCV genotype 2-3 were randomized consecutively into two groups: Treatment group [20 subjects, age 48 ± 14 years, body mass index (BMI) 30 ±...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.3748/wjg.v18.i8.800

    authors: Nimer A,Mouch A

    更新日期:2012-02-28 00:00:00

  • Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis.

    abstract:AIM:To investigate the existence and levels of sH2a, a soluble secreted form of the asialoglycoprotein receptor in human serum. METHODS:Production of recombinant sH2a and development of a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA). This assay was used to determine the presence and concentrati...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v17.i48.5305

    authors: Benyair R,Kondratyev M,Veselkin E,Tolchinsky S,Shenkman M,Lurie Y,Lederkremer GZ

    更新日期:2011-12-28 00:00:00

  • Transcription factor PDX-1 in human colorectal adenocarcinoma: a potential tumor marker?

    abstract:AIM:To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer. METHODS:RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and beni...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.5823

    authors: Ballian N,Liu SH,Brunicardi FC

    更新日期:2008-10-14 00:00:00

  • Risk of postoperative recurrence and postoperative management of Crohn's disease.

    abstract::Crohn's disease (CD) is a chronic inflammatory disease of the digestive tract with systemic manifestations. Etiology is unknown, even if immunological, genetic and environmental factors are involved. The majority of CD patients require surgery during their lifetime due to progressive bowel damage, but, even when all m...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v17.i27.3213

    authors: Spinelli A,Sacchi M,Fiorino G,Danese S,Montorsi M

    更新日期:2011-07-21 00:00:00

  • Idiopathic neonatal pneumoperitoneum with favorable outcome: A case report and review.

    abstract::Neonatal pneumoperitoneum is a surgical emergency indicative of gastrointestinal perforation that requires immediate treatment to prevent death. There have been non-surgical conditions secondary to neonatal pneumoperitoneum (e.g., mechanical ventilation, pulmonary diseases and pneumatosis cystoides intestinalis) that ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v21.i20.6417

    authors: He TZ,Xu C,Ji Y,Sun XY,Liu M

    更新日期:2015-05-28 00:00:00

  • Complementary analysis of microsatellite tumor profile and mismatch repair defects in colorectal carcinomas.

    abstract::Microsatellite instability (MSI) is a prognostic factor and a marker of deficient mismatch repair (MMR) in colorectal adenocarcinomas (CRC). However, a proper application of this marker requires understanding the following: (1) The MSI concept: The PCR approach must amplify the correct locus and accurately identify th...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v12.i37.5932

    authors: Blanes A,Diaz-Cano SJ

    更新日期:2006-10-07 00:00:00

  • Hepatitis B virus and hepatitis C virus dual infection.

    abstract::Hepatitis B virus (HBV) and hepatitis C virus (HCV) share common mode of transmission and both are able to induce a chronic infection. Dual HBV/HCV chronic coinfection is a fairly frequent occurrence, especially in high endemic areas and among individuals at high risk of parenterally transmitted infections. The intrac...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i40.14559

    authors: Caccamo G,Saffioti F,Raimondo G

    更新日期:2014-10-28 00:00:00

  • Primary intestinal non-Hodgkin's lymphoma: a clinicopathologic analysis of 81 patients.

    abstract:AIM:To analyze the clinicopathologic features and the prognosis of primary intestinal lymphoma. METHODS:Patients were included in the study based on standard diagnostic criteria for primary gastrointestinal lymphoma, and were treated at Sun Yat-sen University Cancer Centre between 1993 and 2008. RESULTS:The study com...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v17.i41.4625

    authors: Wang GB,Xu GL,Luo GY,Shan HB,Li Y,Gao XY,Li JJ,Zhang R

    更新日期:2011-11-07 00:00:00

  • Vascular invasion in pancreatic cancer: Imaging modalities, preoperative diagnosis and surgical management.

    abstract::Pancreatic cancer is associated with a poor prognosis, and surgical resection remains the only chance for curative therapy. In the absence of metastatic disease, which would preclude resection, assessment of vascular invasion is an important parameter for determining resectability of pancreatic cancer. A frequent erro...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v16.i7.818

    authors: Buchs NC,Chilcott M,Poletti PA,Buhler LH,Morel P

    更新日期:2010-02-21 00:00:00

  • Acoustic radiation force imaging sonoelastography for noninvasive staging of liver fibrosis.

    abstract:AIM:To investigate the diagnostic accuracy of acoustic radiation force impulse (ARFI) imaging as a noninvasive method for the assessment of liver fibrosis in chronic hepatitis C (CHC) patients. METHODS:We performed a prospective blind comparison of ARFI elastography, APRI index and FibroMax in a consecutive series of ...

    journal_title:World journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3748/wjg.15.5525

    authors: Fierbinteanu-Braticevici C,Andronescu D,Usvat R,Cretoiu D,Baicus C,Marinoschi G

    更新日期:2009-11-28 00:00:00

  • Role of simple biomarkers in predicting fibrosis progression in HCV infection.

    abstract:AIM:To examine the accuracy of the aspartate aminotransferase (AST)/Platelet Ratio Index (APRI) and FIB-4, in predicting longitudinal changes in liver histology in hepatitis C virus (HCV) patients. METHODS:Patients that underwent repeat liver biopsies at least 1 year apart from 1999 to 2007 were identified. Liver fibr...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v16.i45.5710

    authors: Mummadi RR,Petersen JR,Xiao SY,Snyder N

    更新日期:2010-12-07 00:00:00

  • Activation of canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cell line WB-F344 in vitro.

    abstract:AIM:To investigate the effect of activation of canonical Wnt signaling pathway on the proliferation and differentiation of hepatic oval cells in vitro. METHODS:WB-F344 cells were treated with recombinant Wnt3a (20, 40, 80, 160, 200 ng/mL) in serum-free medium for 24 h. Cell proliferation was measured by Brdu incorpora...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.6673

    authors: Zhang Y,Li XM,Zhang FK,Wang BE

    更新日期:2008-11-21 00:00:00