Transcription factor PDX-1 in human colorectal adenocarcinoma: a potential tumor marker?

Abstract:

AIM:To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer. METHODS:RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and benign colon tissue from a single patient. RESULTS:The highest PDX-1 transcription levels were detected in the metastasis material. Lower levels of PDX-1 were found to be present in the primary tumor, while normal colon tissue failed to express detectable levels of PDX-1. Western blot data revealed a PDX-1 expression pattern identical to that of mRNA expression. Immunohistochemistry confirmed high metastasis PDX-1 expression, lower levels in the primary tumor, and the presence of only traces of PDX-1 in normal colon tissue. CONCLUSION:These data argue for further evaluation of PDX-1 as a biomarker for colorectal cancer.

journal_name

World J Gastroenterol

authors

Ballian N,Liu SH,Brunicardi FC

doi

10.3748/wjg.14.5823

subject

Has Abstract

pub_date

2008-10-14 00:00:00

pages

5823-6

issue

38

eissn

1007-9327

issn

2219-2840

journal_volume

14

pub_type

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