Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis.

Abstract:

AIM:To investigate the existence and levels of sH2a, a soluble secreted form of the asialoglycoprotein receptor in human serum. METHODS:Production of recombinant sH2a and development of a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA). This assay was used to determine the presence and concentration of sH2a in human sera of individuals of both sexes and a wide range of ages. RESULTS:The recombinant protein was produced successfully and a specific ELISA assay was developed. The levels of sH2a in sera from 62 healthy individuals varied minimally (147 ± 19 ng/mL). In contrast, 5 hepatitis C patients with cirrhosis showed much decreased sH2a levels (50 ± 9 ng/mL). CONCLUSION:Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals. This constant level and the decrease with cirrhosis suggest a diagnostic potential.

journal_name

World J Gastroenterol

authors

Benyair R,Kondratyev M,Veselkin E,Tolchinsky S,Shenkman M,Lurie Y,Lederkremer GZ

doi

10.3748/wjg.v17.i48.5305

subject

Has Abstract

pub_date

2011-12-28 00:00:00

pages

5305-9

issue

48

eissn

1007-9327

issn

2219-2840

journal_volume

17

pub_type

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