Effect of NDC80 in human hepatocellular carcinoma.

Abstract:

AIM:To investigate the role of nuclear division cycle (NDC)80 in human hepatocellular carcinogenesis. METHODS:NDC80 gene expression was analyzed by real-time reverse transcription polymerase chain reaction in 47 paired hepatocellular carcinoma (HCC) and adjacent tissues. The HCC cell line SMMC-7721 was transfected with lentivirus to silence endogenous NDC80 gene expression, which was confirmed by real-time polymerase chain reaction and western blotting. The effects of NDC80 silencing on SMMC-7721 cell proliferation were evaluated by Cellomics ArrayScan VTI imaging. Cell cycle analysis and apoptosis were detected with flow cytometry. Colony formation was assessed by fluorescence microscopy. RESULTS:NDC80 expression levels in HCC tissues were significantly higher than those in the adjacent tissues. Functional studies demonstrated that NDC80 silencing significantly reduced SMMC-7721 cell proliferation and colony formation. Knockdown of NDC80 resulted in increased apoptosis and cell cycle arrest at S-phase. NDC80 contributed to HCC progression by reducing apoptosis and overcoming cell cycle arrest. CONCLUSION:Elevated expression of NDC80 may play a role in promoting the development of HCC.

journal_name

World J Gastroenterol

authors

Ju LL,Chen L,Li JH,Wang YF,Lu RJ,Bian ZL,Shao JG

doi

10.3748/wjg.v23.i20.3675

subject

Has Abstract

pub_date

2017-05-28 00:00:00

pages

3675-3683

issue

20

eissn

1007-9327

issn

2219-2840

journal_volume

23

pub_type

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