Abstract:
:A current response induced by superfusing adenosine was examined in substantia gelatinosa (SG) neurons of adult rat spinal cord slices by using the whole-cell patch-clamp technique. In 78% of the neurons examined, adenosine induced an outward current at -70 mV [18.8 +/- 1.1 pA (n = 98) at 1mM] in a dose-dependent manner (EC(50) = 177 microM). A similar current was induced by A(1) agonist N(6)-cyclopentyladenosine (1 microM), whereas A(1) antagonist 8-cyclopentyl-1,3-dipropylxanthine (1 microM) reversed the adenosine action. The adenosine current reversed its polarity at a potential being close to the equilibrium potential for K(+), and was attenuated by Ba(2+) (100 microM) and 4-aminopyridine (5mM) but not tetraethylammonium (5mM). The adenosine current was enhanced in duration by equilibrative nucleoside-transport (rENT1) inhibitor S-(4-nitrobenzyl)-6-thioinosine (1 microM) and adenosine deaminase (ADA) inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine (1 microM), and slowed in falling phase by adenosine kinase (AK) inhibitor iodotubercidine (1 microM). We conclude that a Ba(2+)- and 4-aminopyridine-sensitive K(+) channel in SG neurons is opened via the activation of A(1) receptors by adenosine whose level is possibly regulated by rENT1, adenosine deaminase and adenosine kinase. Considering that intrathecally-administered adenosine analogues produce antinociception, the regulatory systems of adenosine may serve as targets for antinociceptive drugs.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Liu T,Fujita T,Kawasaki Y,Kumamoto Edoi
10.1016/j.brainresbull.2004.05.002keywords:
subject
Has Abstractpub_date
2004-07-30 00:00:00pages
75-83issue
1eissn
0361-9230issn
1873-2747pii
S036192300400125Xjournal_volume
64pub_type
杂志文章abstract::Fos and other immediate early gene products are used as markers for neuronal activity. We identified Fos immunocytochemically after KCI-induced depolarization of cultured hypothalamic neurons. Five-day cultures were treated for 1 h with 50 mM KCI or media and fixed at 0, 0.5, 1, 2, and 4 h posttreatment. Sequential im...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(94)00220-u
更新日期:1995-01-01 00:00:00
abstract::Reports from both this laboratory and others indicate that prenatal exposure of rats to cocaine can produce alterations in development, activity and responses to environmental stimuli. In order to determine a biochemical basis for these effects, radioligand receptor-binding assays for different monoaminergic receptors...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(91)90261-h
更新日期:1991-06-01 00:00:00
abstract::The development of the lateral, basomedial and basolateral nuclei of the amygdala in 1-28 day old kittens was studied using Golgi morphometric methods. The major finding of this study is the growth of neurons within each of the nuclei of the amygdala examined occurs during the same time period, between 5-14 and 16-18 ...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(85)90075-9
更新日期:1985-02-01 00:00:00
abstract::IN anesthetized and paralyzed rabbits, action potentials were elicited antidromically following electrical stimulation of optic tract terminals at the geniculate level (LGN). The conduction velocity spectrum extended from 7 m.s-1 to 34 m.s-1. The distribution of conduction velocities indicated four major modes at 10, ...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(80)90207-5
更新日期:1980-11-01 00:00:00
abstract::Progesterone receptor (PR) isoforms expression was determined in several regions of the prepuberal and adult male rat brain by using reverse transcription coupled to polymerase chain reaction. Rats under a 14:10-h light-dark cycle, with lights on at 0600 h were used. We found that in the hypothalamus of prepuberal ani...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(00)00410-x
更新日期:2001-01-01 00:00:00
abstract::The involvement of alpha-amino-3-hydroxy-5-methylizoxazole-4-propionic acid (AMPA) receptors in induction of long-term potentiation (LTP) was examined in rat hippocampal slice preparation. Using conventional extracellular recording, excitatory postsynaptic potentials (EPSPs) and population action potentials (PSs), evo...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(00)00288-4
更新日期:2000-08-01 00:00:00
abstract::The drug delivery across the brain has always been problematic due to the tight junction present namely the blood brain barrier (BBB). BBB only allows selected pharmaceutical drugs to get across it and reach the brain via lipid free diffusion process and exert their therapeutic effect. This leads to the decrease in dr...
journal_title:Brain research bulletin
pub_type: 杂志文章,评审
doi:10.1016/j.brainresbull.2018.08.003
更新日期:2018-09-01 00:00:00
abstract::The function of the phasic dopamine signal, seen in response to salient and rewarding stimuli, has been heavily debated. The reward prediction error hypothesis has been criticised for the suggestion that such a complex signal could be derived at short latencies, relying only on subcortical inputs. However, as more has...
journal_title:Brain research bulletin
pub_type: 杂志文章,评审
doi:10.1016/j.brainresbull.2006.08.010
更新日期:2006-12-11 00:00:00
abstract::The vestibulo-ocular reflex (VOR) has historically been considered a computationally simple reflex: to stabilize images on the retina against imposed head rotation, the eyes must be counterrotated by an equal amount in the opposite direction. During almost any head rotation, however, the eyes are also translated. We s...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(96)00118-9
更新日期:1996-01-01 00:00:00
abstract::To examine synaptic input and association of terminals containing dopamine and other transmitters to rat striatal nitric oxide synthase-expressing neurons, an electron microscopic study using tyrosine hydroxylase (TH) immunohistochemistry combined with histochemistry for NADPH-diaphorase (NADPHd) was performed. NADPHd...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(96)00035-4
更新日期:1996-01-01 00:00:00
abstract::Behavioral adaptations exhibited by two African fossorial mammals for the reception of vibrational signals are discussed. The Namib Desert golden mole (Eremitalpa granti namibensis) is a functionally blind, nocturnal insectivore in the family Chrysochloridae that surface forages nightly in the Namib desert. Both geoph...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(97)00286-4
更新日期:1997-01-01 00:00:00
abstract::Normal human subjects made rapid extensions of the elbow from an initial position to visual targets at different angular distances (9 degrees-54 degrees). We recorded joint angle, acceleration, and surface electromyograms (EMGs) with a monopolar electrode from the triceps and the biceps brachii muscles. Both the agoni...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(93)90034-9
更新日期:1993-01-01 00:00:00
abstract::Fourteen patients with high- (n = 7) and low-dose (n = 7) benzodiazepine (BDZ) dependency presented predominantly with anxious and depressive neurotic symptoms which caused long-term BDZ medication. Their BDZ dependency was characterized by giving preference to the abuse of benzodiazepines with long elimination half-l...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(87)90109-2
更新日期:1987-09-01 00:00:00
abstract::Concomitant behavioral and dorsal raphe (DR) neuronal activity were recorded following acute and chronic dose response of methylphenidate (MPD) in freely moving rats previously implanted with permanent semi-microelectrodes using telemetric (wireless) technology. On experimental day (ED) 1, the neuronal and locomotor a...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2013.06.004
更新日期:2013-09-01 00:00:00
abstract::The thiol reagent cysteamine was administered to adult male rats with the aim of investigating its effect on different neural and pineal components. As expected, immunoreactive somatostatin decreased in the median eminence (ME) (p less than 0.05) and gastric antrum (p less than 0.05) after cysteamine; however, no sign...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(86)90051-1
更新日期:1986-03-01 00:00:00
abstract::Rats received bilateral intracerebroventricular (ICV) infusions of either AF64A (1.5 nmol/ventricle; n = 9) or vehicle (3.0 microliters/ventricle; n = 7). Four weeks later, the animals were anesthetized and their brains processed to visualize and quantify choline acetyltransferase (ChAT) immunoreactive (IR) and parval...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(94)90125-2
更新日期:1994-01-01 00:00:00
abstract::Subcutaneous administration of the alpha 2-adrenoreceptor antagonists, yohimbine and tolazoline, at doses up to 1000 micrograms/kg, had no effect on water intake of female rats. However, when these compounds were administered SC in combination with either the beta-adrenoreceptor agonist, isoproterenol (10 to 25 microg...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(83)90083-7
更新日期:1983-01-01 00:00:00
abstract::We have previously demonstrated that chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) infusion to the substantia nigra (SN) and the locus coeruleus (LC) both produce a long-lasting neurotoxicity on dopamine (DA) and norepinephrine (NE) neurons in these two areas, respectively. In the present study, we furth...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(93)90215-w
更新日期:1993-01-01 00:00:00
abstract::Several studies have demonstrated alterations in the dopamine (DA) system after traumatic brain injury (TBI). Additionally, the existence of significant sex-related differences in the dopaminergic system has long been recognized. Accordingly, the purpose of the present study was to investigate whether TBI would differ...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2016.05.010
更新日期:2016-06-01 00:00:00
abstract::Neutral, magnesium-stimulated sphingomyelinase and acid sphingomyelinase activities in the neurohypophysis, adenohypophysis and cerebrum of rats given 2.25% NaCl drinking water for 7 or 14 days were compared with the same enzyme activities in the tissues of control animals allowed free access to distilled drinking wat...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(83)90028-x
更新日期:1983-05-01 00:00:00
abstract::We have examined the effects of the presence of the mutated human presenilin 1 gene (M146L; hps1*) on lesion-induced sprouting in the hippocampus of the mouse (C57/CBA). The entorhinal cortex was unilaterally lesioned with ibotenic acid in adult, male mice. Four weeks later the subsequent axonal sprouting in the denta...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(01)00751-1
更新日期:2002-03-15 00:00:00
abstract::The role of alpha-2 adrenoceptors in the milk-ejection reflex was investigated by making electrophysiological recordings from oxytocin neurones in the supraoptic nucleus of urethane-anaesthetised rats. Systemic administration of the alpha-2 adrenoceptor antagonist. Idazoxan (0.5 mg/kg, i.v.), temporarily suppressed OT...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(97)00115-9
更新日期:1997-01-01 00:00:00
abstract::Interleukin-33 (IL-33) is closely related to the regulation of immunological cells, and its receptor ST2 is a member of the interleukin-1 (IL-1) receptor family. Inflammatory responses play critical roles in neuronal damage and white matter injury (WMI) post intracerebral hemorrhage (ICH). In this study, we tried to e...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2019.05.016
更新日期:2019-08-01 00:00:00
abstract::Pax6 is an important transcription factor expressed in several discrete domains of the developing central nervous system. It has been reported that Pax6 is involved in the specification of subtypes of hindbrain motor neurons. Pax6 homozygous mutant (rSey2/rSey2) rats die soon after birth, probably due to impaired resp...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2019.07.007
更新日期:2019-10-01 00:00:00
abstract::The effect of serotonin synthesis inhibitor, p-chlorophenylalanine (PCPA), on induction and maintenance of pseudopregnancy as indicated by deciduoma formation was examined in female rats. Animals were injected with 1 mg/kg b.wt. of reserpine on the day of metestrus, and silk thread was passed through and placed in the...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/0361-9230(95)02117-5
更新日期:1996-01-01 00:00:00
abstract::Calcitonin, a peptide hormone expressed in C-cells of the thyreoid gland, as well as capsaicin, isolated from chili, both, modify intracellular signalling in nociceptive neurones. The pathways triggered by the activation of either of these receptors results in a modulation of the intracellular calcium ([Ca(2+)](i)) co...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2011.02.006
更新日期:2011-04-25 00:00:00
abstract::The entopeduncular nucleus (EPN), as the medial part of the globus pallidum, has been considered the efferent structure of the striatum. Based on this, we decided to study its possible somatosensory projections and the electrophysiological relationship with the caudate nucleus (CN). Radial nerve stimulation produced e...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/s0361-9230(81)80050-0
更新日期:1981-03-01 00:00:00
abstract::This work is the second of a series of papers in which we investigated the neurophysiological basis of deep brain stimulation (DBS) clinical efficacy using post-operative local field potential (LFP) recordings from DBS electrodes implanted in the subthalamic nucleus (STN) in patients with Parkinson's disease. We found...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2006.08.015
更新日期:2006-12-11 00:00:00
abstract::Changes in dietary habits, including the increased consumption of processed foods, rich in trans fatty acids (TFA), have profound effects on offspring health in later life. Thus, this study aimed to assess the influence of maternal trans fat intake during pregnancy or lactation on anxiety behavior, as well as markers ...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2020.11.016
更新日期:2021-01-01 00:00:00
abstract::In a previous study we showed that healthy highly hypnotizable subjects, during the suggestion of a moderately unpleasant situation administered in awake conditions, exhibited a sympathetic response greatly attenuated with respect to non-hypnotizable individuals. This was interpreted as a natural protection of hypnoti...
journal_title:Brain research bulletin
pub_type: 杂志文章
doi:10.1016/j.brainresbull.2005.12.003
更新日期:2006-03-31 00:00:00