Abstract：
:Fos and other immediate early gene products are used as markers for neuronal activity. We identified Fos immunocytochemically after KCI-induced depolarization of cultured hypothalamic neurons. Five-day cultures were treated for 1 h with 50 mM KCI or media and fixed at 0, 0.5, 1, 2, and 4 h posttreatment. Sequential immunocytochemistry was performed to identify Fos immunoreactivity in tyrosine hydroxylase (TH)-immunoreactive (-ir) or oxytocin (OT)-ir neurons. Activated neurons [brown cells (TH-ir or OT-ir) with purple nuclei (Fos-ir)] were counted microscopically. KCI treatment resulted in an increased percentage of Fos-ir TH-ir and OT-ir neurons over control levels over the time course examined. Fos-ir peaked in TH-ir neurons at 0.5 to 1 h posttreatment, with levels returning to baseline at 4 h. Fos-ir in OT-ir neurons peaked at 2 h, and remained elevated at 4 h, showing prolonged activation. These results demonstrate that KCI-induced depolarization of cultured hypothalamic neurons increases Fos with a different time course in TH-ir vs. OT-ir neurons.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Sim LJ,Morris Mdoi
10.1016/0361-9230(94)00220-usubject
Has Abstractpub_date
1995-01-01 00:00:00pages
399-404issue
4eissn
0361-9230issn
1873-2747pii
0361-9230(94)00220-Ujournal_volume
36pub_type
杂志文章abstract：:Neurochemical analysis of norepinephrine and serotonin with high performance liquid chromatography with electrochemical detection (LCEC) in several microdissected central autonomic nuclei of 4 week old and 16 week old spontaneously hypertensive rats (SHR), normotensive Wistar-Kyoto rats (WKY), and control normotensive...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(84)90095-9
更新日期：1984-09-01 00:00:00
abstract：:The olfactory bulbectomized (OBX) rat model of depression has been widely used in studies on the behavioral and neurochemical aspects of human depression. The objective of the present investigation was to assess open field (OF) activity and the brain regional 5-HT(1A) receptor densities of the sham operated (SHX) and ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2007.09.005
更新日期：2008-03-28 00:00:00
abstract：:Recent findings in epigenetics shed new light on the regulation of gene expression in the central nervous system (CNS) during stress. The most frequently studied epigenetic mechanisms are DNA methylation, histone modifications and microRNA activity. These mechanisms stably determine cell phenotype but can also be resp...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2013.07.003
更新日期：2013-09-01 00:00:00
abstract：:The possible existence of two separate RSA generators in the dorsal hippocampus raises questions as to the degree of coupling or independence of both. In order to answer these questions bipolar EEG recording were made in the CA1 and dentate gyrus of freely moving rats during conditions of RSA generation by different m...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(81)80063-9
更新日期：1981-01-01 00:00:00
abstract：:In the present study we investigated the effect of electrolytic lesion of the medial septal area (MSA) on the pressor and dipsogenic response to cholinergic activation and angiotensin II (ANGII) injection into the subfornical organ (SFO) in rats. In addition the effect of MSA lesion on the natriuresis, kaliuresis and ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90191-0
更新日期：1994-01-01 00:00:00
abstract：:Astrocytes become activated and proliferate in response to autoimmune diseases involving the CNS. In many cases, elevated numbers of astrocytes may compound inflammatory responses and exacerbate neuronal degeneration. The regulation of astrocytes in disease states has been shown as an important corollary to disease pr...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2012.09.001
更新日期：2013-01-01 00:00:00
abstract：:The antinociceptive response of mice to the amino acid L-arginine (L-ARG) has been attributed to either an opioid mechanism or a non-opioid but nitric oxide (NO)-dependent mechanism. Earlier it was reported that the mechanism of nitrous oxide-induced antinociception involved opioid components and was also dependent on...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.05.008
更新日期：2006-07-31 00:00:00
abstract：:Levels of serotonin were measureed in microdissected, individual brain nuclei in ovariectomized rats after treatment with ovarian hormones. Regions sampled included nuclei in the forebrain, rostral and medial hypothalamus, and midbrain tegmentum. Estradiol benzoate decreased levels of luteinizing hormone in plasma but...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(79)90044-3
更新日期：1979-07-01 00:00:00
abstract：:The effect of lithium chloride on the activity of the afferent nerve fibers from the abdominal organs was studied in anesthetized rats. The administration of the lithium chloride solution (0.15 M) over the mesentery increased afferent discharge rate of the splanchnic and vagal gastric nerve. Injection of the same solu...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90094-9
更新日期：1994-01-01 00:00:00
abstract：:Glutamate afferents reaching the ventral tegmental area (VTA) affect dopamine (DA) cells in this structure probably mainly via NMDA receptors. VTA appears to be one of the structures involved in regulation of hippocampal theta rhythm, and this work aimed at assessing the role of glutamatergic activation of the VTA in ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2014.06.001
更新日期：2014-08-01 00:00:00
abstract：:Fenfluramine, an anorexigenic drug, lowers serotonin (5-hydroxytryptamine) and 5-hydroxyindoleacetic acid levels in brain, spinal fluid, and blood, and has been used as a treatment for autism. Fenfluramine's mode of action is unknown. We present evidence from chromatography and nuclear magnetic resonance spectroscopy ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(84)90209-0
更新日期：1984-01-01 00:00:00
abstract：:In post-natal kittens which were given injections of HRP in the suprasylvian gyrus (polysensory cortex), the cortico-cortical connections were studied qualitatively and quantitatively. These connections originate essentially from cortical cells localized in cortices of the upper part of the hemisphere. They are formed...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(85)90040-1
更新日期：1985-11-01 00:00:00
abstract：:This paper reviews and provides new data on the relationship of the peptide content in rat dorsal root ganglion (DRG) neurons to a) the neurofilament content of the soma and b) the conduction velocities of the fibres. The latter involved intracellular recordings made in vitro followed by dye injection and immunocytoch...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90250-f
更新日期：1993-01-01 00:00:00
abstract：:Injection of colchicine into the lateral ventricles (ICV) of rats results in a selective loss of neurons immunoreactive for choline acetyltransferase (ChAT) in the medial septum (MS) and a concomitant loss of acetylcholinesterase (AChE) positive fibers in the hippocampus. To determine if this loss of cholinergic cells...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(92)90132-h
更新日期：1992-11-01 00:00:00
abstract：:Secondary brain injuries following intracerebral hemorrhage (ICH) are mediated by inflammatory pathway activation. The present study aimed to characterize long noncoding RNAs (lncRNAs) that are differentially expressed in cerebral tissues during ICH pathogenesis and to investigate their pathogenic functions. An ICH mo...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.04.017
更新日期：2020-09-01 00:00:00
abstract：:Postmortem caudate and substantia nigra tissue samples from human parkinsonian patients (PD) and age-matched controls (NC) were analyzed for uric acid (UA), dopamine (DA), and ascorbic acid (AA) by HPLC/UV/ED. Uric acid and DA levels were significantly lower in the substantia nigra of PD by 54% and 85%, respectively. ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90285-2
更新日期：1994-01-01 00:00:00
abstract：:Cerebral ischemia is one of the leading causes of death and long-term disability worldwide. N-myc downstream-regulated gene 4 (NDRG4) is predominantly expressed in the brain as well as in the heart and has been reported to be involved in resistance to neuronal cell death caused by ischemic injury. However, the underly...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.12.010
更新日期：2019-03-01 00:00:00
abstract：:We studied levels of mRNA for the alpha- and beta-subunits of calmodulin (CaM) kinase II using the amygdaloid kindling model of epilepsy. There were significant increases in mRNA for the beta-subunit of CaM kinase II in the hippocampus 4-24 h after stage 5-kindled seizures. Moreover, this mRNA was significantly increa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(97)00022-1
更新日期：1997-01-01 00:00:00
abstract：:It has been demonstrated that the posteromedial cortical amygdaloid nucleus (PMCo), is sexually dimorphic. It is shown (Experiment 1) that male orchidectomy on the day of birth (D1) decreases the volume and number of neurons of the PMCo, while a single injection of propionate testosterone to the female on D1 masculini...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(00)00332-4
更新日期：2000-10-01 00:00:00
abstract：:Normal human subjects made rapid extensions of the elbow from an initial position to visual targets at different angular distances (9 degrees-54 degrees). We recorded joint angle, acceleration, and surface electromyograms (EMGs) with a monopolar electrode from the triceps and the biceps brachii muscles. Both the agoni...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90034-9
更新日期：1993-01-01 00:00:00
abstract：:Hypothalamic-pituitary-adrenal (HPA) responses to single-dose nicotine (NIC) are sexually diergic: Female rats have higher adrenocorticotropic hormone (ACTH) and corticosterone (CORT) responses than do males. In the present study we determined HPA responses in male and female rats following single doses of NIC, a sing...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2011.03.001
更新日期：2011-05-30 00:00:00
abstract：:Chronic brain implants are accompanied by a tissue response that causes the loss of neurons in the vicinity of the implant and the formation of a glial scar that is also referred to as foreign body response. Despite immense progress in the field of brain-computer interface (BCI) research the biocompatibility of chroni...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2015.08.001
更新日期：2015-08-01 00:00:00
abstract：:Chronic cerebral hypofusion (CCH) after bilateral carotid artery occlusion (2VO) causes cognitive damage and neuronal degeneration in the cortex and hippocampal CA1 area, and influences the oxygen and glucose supply in the brain which often results in metabolic alterations and oxidative stress. AMP-activated protein k...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.03.019
更新日期：2018-06-01 00:00:00
abstract：:Most of the lateral hypothalamic neurons responding to moderate changes in blood glucose fail to be affected by direct glucose applications. Therefore their sensitivity to glycemic level must be mediated by an indirect mechanism. In order to test whether adrenergic afferents might be involved, the activity of lateral ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(91)90086-y
更新日期：1991-04-01 00:00:00
abstract：:Microglial and astroglial cells undergo prompt responses to peripheral motor and sensory axon injury. These responses include proliferation of microglial cells as well as hypertrophy and increased levels of glial fibrillary acidic protein around the axotomized motoneurons and in the central projection territories of p...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/0361-9230(93)90284-i
更新日期：1993-01-01 00:00:00
abstract：:The volume and neuron number of the sexually dimorphic accessory olfactory bulb and locus coeruleus are altered by early postnatal exposure (from the day of birth to postnatal day 16) to diazepam. After diazepam treatment, both volume and neuron number were decreased in the male accessory olfactory bulb and in the fem...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(91)90255-i
更新日期：1991-06-01 00:00:00
abstract：:Nagase genetically analbuminemic rats (NAR) were run to fatigue. Administration of branched chain amino acids (BCAA) before exhaustive exercise, resulted in a post-fatigue decreased tryptophan uptake (-22%, p < 0.05) and 5-hydroxytryptophan (5-HTP) uptake (-29%, p < 0.01) into the synaptosomes isolated from the striat...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(99)00276-2
更新日期：2000-05-01 00:00:00
abstract：:We studied the effects of estradiol (E2) and progesterone (P) on progesterone receptor (PR) isoforms gene expression in the brain of ovariectomised female and gonadectomised male rats by RT-PCR analysis. In female rats, the expression of both PR isoforms was induced by E2 and down-regulated by P in the hypothalamus, w...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(02)00845-6
更新日期：2002-10-30 00:00:00
abstract：:The brain serotoninergic (5HTergic) system of epileptic subjects can influence their vulnerability to stress. We studied the putative dependency of 5HT neurotransmission parameters on emotional stress, and the presence, types and severity of seizures using rats with genetic generalized (absence and/or audiogenic) epil...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.03.008
更新日期：2006-05-31 00:00:00
abstract：:The effects of intraseptally microinjected muscarinic (atropine sulfate, pirenzepine and gallamine) and nicotinic (hexamethonium) antagonists on spontaneous, sensory and electrically-induced hippocampal (HPC) theta EEG activity were investigated in the freely behaving cat. Administration of hexamethonium failed to eli...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(02)00797-9
更新日期：2002-07-01 00:00:00
