A protocol for shoot multiplication from foliar meristem of Vanda spathulata (L.) Spreng.

Abstract:

:Leaf explants collected from flowering plants of Vanda spathulata were cultured in Mitra medium with combinations of 6-benzyladenine (BA; 13.2-88.8 microM) and indole-3-acetic acid (IAA; 0.0 -85.6 microM). Combination of BA (66.6 microM) and IAA (28.5 microM) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf explants. For optimized multiplication, BA:IAA (2:1 microM) was essential at 22.2- 88.8 microM of BA. Re-cultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 microM of BA and 5.7-28.5 microM of IAA. Rooting of shoots (> 95%) occurred in medium containing banana pulp (75 gl(-1)) and IAA (5.7 microM) within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.

journal_name

Indian J Exp Biol

authors

Decruse SW,Gangaprasad A,Seeni S,Menon VS

keywords:

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

924-7

issue

8

eissn

0019-5189

issn

0975-1009

journal_volume

41

pub_type

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