Abstract:
:Previous studies point to protein kinase C (PKC) isozyme eta as a resistance factor in cancer cells. Therefore, we investigated whether down-regulation of PKCeta with second generation antisense oligonucleotides (ODNs) would sensitise A549 human lung carcinoma cells to cytostatics. The effects were compared to the outcome of Bcl-xL down-regulation. Upon treatment with antisense ODNs, PKCeta and Bcl-xL were both significantly reduced on mRNA and protein level. Down-regulation of either PKCeta or Bcl-xL in combination with vincristine or paclitaxel resulted in a significant increase in caspase-3 activity compared to that in the control oligonucleotide treated cells. In addition, PKCeta down-regulation augmented vincristine-induced dissipation of mitochondrial transmembrane potential. In conclusion, these results confirm that PKCeta might represent a considerable resistance factor and an interesting target to improve anticancer chemotherapy.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Sonnemann J,Gekeler V,Ahlbrecht K,Brischwein K,Liu C,Bader P,Müller C,Niethammer D,Beck JFdoi
10.1016/j.canlet.2004.02.001keywords:
subject
Has Abstractpub_date
2004-06-25 00:00:00pages
177-85issue
2eissn
0304-3835issn
1872-7980pii
S0304383504001399journal_volume
209pub_type
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