Synthesis of ribozyme against vascular endothelial growth factor165 and its biological activity in vitro.


AIM:To investigate the designation, synthesis and biological activity of against vascular endothelial growth factor165 (VEGF165) ribozyme. METHODS:The ribozyme against VEGF165 was designed with computer. The transcriptional vector was constructed which included the anti-VEGF165 ribozyme and 5', 3' self-splicing ribozymes. The hammerhead ribozyme and substrate VEGF165 mRNA were synthesized through transcription in vitro. The cleavage activity of the ribozyme on target RNA was observed in a cell-free system. RESULTS:The anti-VEGF165 ribozyme was released properly from the transcription of pGEMRz212 cleaved by 5' and 3' self-splicing ribozymes which retained its catalytic activity, and the cleavage efficiency of ribozyme reached 90.7%. CONCLUSION:The anti-VEGF165 ribozyme designed with computer can cleave VEGF165 mRNA effectively.


World J Gastroenterol


Gu ZP,Wang YJ,Wu Y,Li JG,Chen NA





Has Abstract


2004-05-15 00:00:00












  • Effect of early preoperative 5-fluorouracil on the integrity of colonic anastomoses in rats.

    abstract:AIM:To determine the effect of chemotherapy on wound healing by giving early preoperative 5-fluorouracil (5-FU) to rats with colonic anastomoses. METHODS:Sixty Albino-Wistar male rats (median weight, 235 g) were used in this study. The rats were fed with standard laboratory food and given tap water ad libitum. The ani...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Ozel L,Ozel MS,Toros AB,Kara M,Ozkan KS,Tellioglu G,Krand O,Koyuturk M,Berber I

    更新日期:2009-09-07 00:00:00

  • Endoscopic papillectomy of minor papillar adenoma associated with pancreas divisum.

    abstract::Tumors of the minor papilla of the duodenum are quite rare. We successfully and safely treated an 18-mm adenoma of the minor papilla associated with pancreas divisum using endoscopic papillectomy. A 64-year-old man was admitted to our hospital for treatment of an asymptomatic mass in the minor papilla detected by uppe...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Kanamori A,Kumada T,Kiriyama S,Sone Y,Tanikawa M,Hisanaga Y,Toyoda H,Kawashima H,Itoh A,Hirooka Y,Goto H

    更新日期:2009-03-07 00:00:00

  • Ulcerative colitis patients in clinical remission demonstrate correlations between fecal immunochemical test results, mucosal healing, and risk of relapse.

    abstract:AIM:To assess the risk of relapse in ulcerative colitis (UC) patients in clinical remission using mucosal status and fecal immunochemical test (FIT) results. METHODS:The clinical outcomes of 194 UC patients in clinical remission who underwent colonoscopy were based on evaluations of Mayo endoscopic subscores (MESs) an...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Nakarai A,Kato J,Hiraoka S,Takashima S,Takei D,Inokuchi T,Sugihara Y,Takahara M,Harada K,Okada H

    更新日期:2016-06-07 00:00:00

  • Expression of somatostatin mRNA in various differentiated types of gastric carcinoma.

    abstract::AIM:To investigate the expression of somatostatin mRNA in various differentiated types of gastric carcinoma.METHODS: By using in situ hybridization and immunohistochemical techniques, the expression of somatostatin mRNA and somatostatin immunoreactivity in the normal gastric mucosa, the poorly, moderately and well-dif...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Zhang QX,Dou YL,Shi XY,Ding Y

    更新日期:1998-02-01 00:00:00

  • Pediatric non-alcoholic fatty liver disease: Recent solutions, unresolved issues, and future research directions.

    abstract::Non-alcoholic fatty liver disease (NAFLD) in children is becoming a major health concern. A "multiple-hit" pathogenetic model has been suggested to explain the progressive liver damage that occurs among children with NAFLD. In addition to the accumulation of fat in the liver, insulin resistance (IR) and oxidative stre...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Clemente MG,Mandato C,Poeta M,Vajro P

    更新日期:2016-09-28 00:00:00

  • Current clinical approach to achalasia.

    abstract::Idiopathic achalasia is a rare primary motility disorder of the esophagus. The classical features are incomplete relaxation of a frequently hypertensive lower esophageal sphincter (LES) and a lack of peristalsis in the tubular esophagus. These motor abnormalities lead to dysphagia, stasis, regurgitation, weight loss, ...

    journal_title:World journal of gastroenterology

    pub_type: 社论


    authors: Eckardt AJ,Eckardt VF

    更新日期:2009-08-28 00:00:00

  • Second-line bismuth-containing quadruple therapy for Helicobacter pylori eradication and impact of diabetes.

    abstract:AIM:To investigate Helicobacter pylori (H. pylori) eradication rates using second-line bismuth-containing quadruple therapy and to identify predictors of eradication failure. METHODS:This study included 636 patients who failed first-line triple therapy and received 7 d of bismuth-containing quadruple therapy between J...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Kim SE,Park MI,Park SJ,Moon W,Kim JH,Jung K,Kim HK,Lee YD

    更新日期:2017-02-14 00:00:00

  • Dilemma of first line regimens in metastatic pancreatic adenocarcinoma.

    abstract::Pancreatic cancer is one of the deadliest cancers, ranking fourth among cancer-related deaths. Despite all the major molecular advances and treatment breakthroughs, mainly targeted therapies, the cornerstone treatment of metastatic pancreatic cancer (mPC) remains cytotoxic chemotherapy. In 2016, more than 40 years aft...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Ghosn M,Ibrahim T,Assi T,El Rassy E,Kourie HR,Kattan J

    更新日期:2016-12-14 00:00:00

  • Incidence and mortality of primary liver cancer in England and Wales: changing patterns and ethnic variations.

    abstract:AIM:To explore recent trends, modes of diagnosis, ethnic distribution and the mortality to incidence ratio of primary liver cancer by subtypes in England and Wales. METHODS:We obtained incidence (1979-2008) and mortality (1968-2008) data for primary liver cancer for England and Wales and calculated age-standardised in...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Ladep NG,Khan SA,Crossey MM,Thillainayagam AV,Taylor-Robinson SD,Toledano MB

    更新日期:2014-02-14 00:00:00

  • Overview and comparison of guidelines for management of pancreatic cystic neoplasms.

    abstract::Pancreatic cysts are identified at an increasing frequency. Although mucinous cystic neoplasms represent a pre-malignant condition, the majority of these lesions do not progress to cancer. Over the last 10 years several societies have established guidelines for the diagnosis, initial evaluation and surveillance of the...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Hasan A,Visrodia K,Farrell JJ,Gonda TA

    更新日期:2019-08-21 00:00:00

  • Vascular tumors and malformations of the colon.

    abstract::The term "hemangioma" refers to the common tumor of infancy that exhibits rapid postnatal growth and slow regression during childhood. It may cause confusion with venous malformations that are often incorrectly called "cavernous hemangioma". Venous malformations comprise abnormally formed channels that are lined by qu...

    journal_title:World journal of gastroenterology

    pub_type: 评论,信件


    authors: Fernandez-Pineda I

    更新日期:2009-11-07 00:00:00

  • Mutations in components of the Wnt signaling pathway in gastric cancer.

    abstract:AIM:To explore the contribution of AXIN1, AXIN2 and beta-catenin, components of Wnt signaling pathway, to the carcinogenesis of gastric cancer (GC), we examined AXIN1, AXIN2 exon7 and CTNNB1 (encoding beta-catenin) exon3 mutations in 70 GCs. METHODS:The presence of mutations was identified by polymerase chain reaction...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Pan KF,Liu WG,Zhang L,You WC,Lu YY

    更新日期:2008-03-14 00:00:00

  • Advanced imaging techniques in the therapeutic response of transarterial chemoembolization for hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is one of the major causes of morbidity and mortality in patients with chronic liver disease. Transarterial chemoembolization (TACE) can significantly improve the survival rate of patients with HCC and is the first treatment choice for patients who are not suitable for surgical resection...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Yang K,Zhang XM,Yang L,Xu H,Peng J

    更新日期:2016-05-28 00:00:00

  • Epidemiology and natural history of hepatitis C virus infection.

    abstract::Hepatitis C virus (HCV) affects 130-210 million people worldwide and is one of the major risk factors for hepatocellular carcinoma. Globally, at least one third of hepatocellular carcinoma cases are attributed to HCV infection, and 350000 people died from HCV related diseases per year. There is a great geographical va...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Lee MH,Yang HI,Yuan Y,L'Italien G,Chen CJ

    更新日期:2014-07-28 00:00:00

  • Resveratrol and fenofibrate ameliorate fructose-induced nonalcoholic steatohepatitis by modulation of genes expression.

    abstract:AIM:To evaluate the effect of resveratrol, alone and in combination with fenofibrate, on fructose-induced metabolic genes abnormalities in rats. METHODS:Giving a fructose-enriched diet (FED) to rats for 12 wk was used as a model for inducing hepatic dyslipidemia and insulin resistance. Adult male albino rats (150-200 ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Abd El-Haleim EA,Bahgat AK,Saleh S

    更新日期:2016-03-14 00:00:00

  • Incidence and clinical features of endoscopic ulcers developing after gastrectomy.

    abstract:AIM:To determine the precise incidence and clinical features of endoscopic ulcers following gastrectomy. METHODS:A consecutive series of patients who underwent endoscopic examination following gastrectomy between 2005 and 2010 was retrospectively analyzed. A total of 78 patients with endoscopic ulcers and 759 without ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Chung WC,Jeon EJ,Lee KM,Paik CN,Jung SH,Oh JH,Kim JH,Jun KH,Chin HM

    更新日期:2012-07-07 00:00:00

  • Effects of herbal compound 861 on human hepatic stellate cell proliferation and activation.

    abstract:AIM:To investigate the effects of herbal compound 861 (Cpd 861) on cell proliferation in human hepatic stellate cells (LX-2) and human hepatocellular liver carcinoma cells (HepG2), and expression of alpha-smooth muscle actin (alpha-SMA) in LX-2 cells. METHODS:LX-2 and HepG2 cells were incubated with various concentrat...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Wang L,Wang J,Wang BE,Xiao PG,Qiao YJ,Tan XH

    更新日期:2004-10-01 00:00:00

  • Does antioxidant therapy influence every aspect of quality of life?

    abstract::To present problems that might severely impact the conclusions drawn by the authors of an article on antioxidant treatment in chronic pancreatitis (World J Gastroenterol 2010; 16: 4066-4071). We analyzed and discussed this paper by Shah et al, and found that promising as it is, this study has some methodological short...

    journal_title:World journal of gastroenterology

    pub_type: 评论,信件


    authors: Stefaniak T,Vingerhoets A,Sledzinski Z

    更新日期:2012-05-21 00:00:00

  • Endoscopic ultrasound-guided biliary drainage: Bilateral systems drainage via left duct approach.

    abstract::Endoscopic ultrasound (EUS)-guided biliary drainage is accepted as a less invasive, alternative treatment for patients in whom endoscopic retrograde cholangiopancreatography has failed. Most patients with malignant hilar obstruction undergo EUS-guided hepaticogastrostomy. The authors present the case of a 77-year-old ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Prachayakul V,Aswakul P

    更新日期:2015-09-14 00:00:00

  • Association between MDM2-SNP309 and hepatocellular carcinoma in Taiwanese population.

    abstract:AIM:To investigate the risk association and compare the onset age of hepatocellular carcinoma (HCC) patients in Taiwan with different genotypes of MDM2-SNP309. METHODS:We analyzed MDM2-SNP309 genotypes from 58 patients with HCC and 138 cancer-free healthy controls consecutively. Genotyping of MDM2-SNP309 was conducted...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Leu JD,Lin IF,Sun YF,Chen SM,Liu CC,Lee YJ

    更新日期:2009-11-28 00:00:00

  • Seven synchronous early gastric cancer with 28 lymph nodes metastasis.

    abstract::An 85 year male patient complaining epigastric discomfort was admitted. From the esophagogastroduodenoscopy, three early gastric cancer (EGCa) lesions had been identified and these were diagnosed as adenocarcinoma with poorly differentiated cell type. The patient underwent operation. From the post-operative mapping, h...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Seong H,Kim JI,Lee HJ,Kim HJ,Cho HJ,Kim HK,Cheung DY,Kim DJ,Kim W,Kim TJ

    更新日期:2013-11-28 00:00:00

  • Confocal laser endomicroscopy and immunoendoscopy for real-time assessment of vascularization in gastrointestinal malignancies.

    abstract::Gastrointestinal cancers represent a major cause of morbidity and mortality, with incomplete response to chemotherapy in the advanced stages and poor prognosis. Angiogenesis plays a crucial part in tumor growth and metastasis, with most gastrointestinal cancers depending strictly on the development of a new and devote...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Gheonea DI,Cârţână T,Ciurea T,Popescu C,Bădărău A,Săftoiu A

    更新日期:2011-01-07 00:00:00

  • MicroRNAs potential utility in colon cancer: Early detection, prognosis, and chemosensitivity.

    abstract::Over the past decade, research has shown that aberrant expression of microRNA (miRNA) is involved in colorectal cancer development and progression. MicroRNAs are small sequences of non-coding RNA that regulate expression of genes involved in important cellular functions, such as cell differentiation, multiplication, a...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Hollis M,Nair K,Vyas A,Chaturvedi LS,Gambhir S,Vyas D

    更新日期:2015-07-21 00:00:00

  • Recombinant Helicobacter pylori catalase.

    abstract:AIM:To construct a recombinant strain which highly expresses catalase of Helicobacter pylori (H. pylori) and assay the activity of H. pylori catalase. METHODS:The catalase DNA was amplified from H. pylori chromosomal DNA with PCR techniques and inserted into the prokaryotie expression vector pET-22b (+), and then was ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Bai Y,Zhang YL,Jin JF,Wang JD,Zhang ZS,Zhou DY

    更新日期:2003-05-01 00:00:00

  • Quality of life: A critical outcome for all surgical treatments of gastric cancer.

    abstract::Surgery represents the main curative therapeutic modality for gastric cancer, and it is occasionally considered for palliation as well as prophylaxis. Most frequently, surgical outcomes are conveyed in terms of oncological outcomes such as recurrence and survival. However, quality of life (QoL) is also important and s...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: McCall MD,Graham PJ,Bathe OF

    更新日期:2016-01-21 00:00:00

  • Protective effect and mechanism of stronger neo-minophagen C against fulminant hepatic failure.

    abstract:AIM:To investigate the protective effect of stronger neo-minophafen C (SNMC) on fulminant hepatic failure (FHF) and its underlying mechanism. METHODS:A mouse model of FHF was established by intraperitoneal injection of galactosamine (D-Gal N) and lipopolysaccharide (LPS). The survival rate, liver function, inflammator...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Yang BS,Ma YJ,Wang Y,Chen LY,Bi MR,Yan BZ,Bai L,Zhou H,Wang FX

    更新日期:2007-01-21 00:00:00

  • Increased susceptibility of aging gastric mucosa to injury: the mechanisms and clinical implications.

    abstract::This review updates the current views on aging gastric mucosa and the mechanisms of its increased susceptibility to injury. Experimental and clinical studies indicate that gastric mucosa of aging individuals-"aging gastropathy"-has prominent structural and functional abnormalities vs young gastric mucosa. Some of thes...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Tarnawski AS,Ahluwalia A,Jones MK

    更新日期:2014-04-28 00:00:00

  • Metabolic inflammation as an instigator of fibrosis during non-alcoholic fatty liver disease.

    abstract::Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive storage of fatty acids in the form of triglycerides in hepatocytes. It is most prevalent in western countries and includes a wide range of clinical and histopathological findings, namely from simple steatosis to steatohepatitis and fibrosis, which...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Katsarou A,Moustakas II,Pyrina I,Lembessis P,Koutsilieris M,Chatzigeorgiou A

    更新日期:2020-05-07 00:00:00

  • Locally advanced undifferentiated carcinoma with osteoclast-like giant cells of the pancreas.

    abstract::Undifferentiated carcinoma with osteoclast-like giant cells of the pancreas (UCOGCP) is an unusual pancreatic neoplasm that represents < 1% of all pancreatic malignancies. Moreover, the giant cells of UCOGCP morphologically resemble the benign giant cells of bone tumors. Due to the rarity of this tumor type, the histo...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Gao HQ,Yang YM,Zhuang Y,Liu P

    更新日期:2015-01-14 00:00:00

  • Hypoxia inducible factor-1alpha mediates protective effects of ischemic preconditioning on ECV-304 endothelial cells.

    abstract:AIM:To investigate whether hypoxia inducible factor-1alpha (HIF-1alpha) is linked to the protective effects of ischemic preconditioning (IP) on sinusoidal endothelial cells against ischemia/reperfusion injury. METHODS:Sinusoidal endothelial cell lines ECV-304 were cultured and divided into four groups: control group, ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Shi LB,Huang JH,Han BS

    更新日期:2007-04-28 00:00:00