Abstract:
AIM:To establish a quantitative method to measure the amount of lipids. METHODS:The livers of 53 male Wistar rats (225 g) with different degrees of hepatic steatosis were studied. This model of hepatic steatosis was based on a high carbohydrate, fat-free modified diet. Biopsies were classified into four grades depending on fat accumulation, using the Kleiner and Brunt classification. Total fat was studied by the Soxtec method (Soxtec 2050 Auto Fat Extraction System), and agreement between both assays was assessed by calculating the kappa coefficient. RESULTS:According to the histological classification, 38% of rats presented grade 0, 21% grade 1, 22% grade 2 and 20% grade 3. The amount of fat per 100 g tissue was 2.60 +/- 0.64 g for grade 0, 3.87 +/- 1.59 g for grade 1, 5.82 +/- 1.37 g for grade 2 and 8.68 +/- 2.30 g for grade 3. Statistically significant differences were found between the mean values for each of the histological grades (P < 0.05). The correlation for the quantification of fat in the liver between both assays was moderate (kappa = 0.60). CONCLUSION:The biochemical quantification of fat in liver tissue by the Soxtec method was correlated with the histological classification, although the agreement between the two tests was only moderate.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Hijona E,Hijona L,Larzabal M,Sarasqueta C,Aldazabal P,Arenas J,Bujanda Ldoi
10.3748/wjg.v16.i12.1495subject
Has Abstractpub_date
2010-03-28 00:00:00pages
1495-9issue
12eissn
1007-9327issn
2219-2840journal_volume
16pub_type
杂志文章abstract::We report a rare case of hypereosinophilic syndrome (HES) presenting with intractable gastric ulcers. A 71-year-old man was admitted with epigastric pain. Initial endoscopic findings revealed multiple, active gastric ulcers in the gastric antrum. He underwent Helicobacter pylori (H pylori) eradication therapy followed...
journal_title:World journal of gastroenterology
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,多中心研究
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journal_title:World journal of gastroenterology
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pub_type: 杂志文章,评审
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pub_type: 社论,评审
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pub_type: 杂志文章,meta分析,评审
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更新日期:2013-12-28 00:00:00