Abstract:
:Lipopolysaccharide (LPS [endotoxin]), a structural component of gram-negative bacteria, is implicated in the pathogenesis of septic shock. Lipid A is an evolutionarily conserved region of LPS that has been identified as the toxic component of LPS. Therapeutic strategies for the treatment of septic shock in humans are currently focused on neutralization of LPS. Here, the anti-endotoxin activity of BNEP, a synthetic peptide derived from the human bactericidal/permeability-increasing protein (BPI; aa 148-161) was investigated in vitro and in experimental animal endotoxemia models in vivo. The ability of BNEP to bind LPS from Escherichia coli O55:B5 and lipid A from Salmonella Re 595 was tested using an affinity sensor assay, and its ability to neutralize LPS was tested using a sensitive Limulus amebocyte lysate (LAL) assay. Polymyxin B (PMB) was used as the positive control in the in vitro experiments and in mouse experiments. We found that BNEP and PMB bound LPS with a similar affinity (Kd values of 25.4 and 25.8 nM, respectively). In contrast, BNEP bound lipid A with a slightly lower affinity than that of PMB (Kd values of 8 and 5.6 nM, respectively). The exact capacity of BNEP binding to LPS was approximately 0.53 microg peptide per 1 ng of LPS, as shown by affinity sensor assay. The LAL test showed that 256 microg of BNEP almost completely neutralized 2 ng LPS. In vivo, mice were randomized, intravenously injected with BNEP (0.5-10 mg/kg) or 1 mg/kg PMB, and then lethally challenged with 20 mg/kg LPS. We found that 5 mg/kg BNEP significantly protected mice from LPS challenge. In an endotoxemia rat model, animals were co-treated with 5 or 10 mg/kg BNEP and 10 mg/kg LPS via cardiac catheter. BNEP treatment resulted in significant reduction of tumor necrosis factor alpha (TNF-alpha) and IL-6, compared with LPS-only control animals. In addition, 10 mg/kg BNEP-treated animals showed a significant decrease in plasma endotoxin levels in comparison to animals treated with LPS alone. These results provide evidence that BNEP effectively neutralizes LPS in vitro and in vivo, and could protect animals from the lethal effects of LPS via decreasing plasma endotoxin and proinflammatory cytokines. Our work suggests that this peptide is worthy of further investigation as a possible novel treatment for septic shock.
journal_name
Int Immunopharmacoljournal_title
International immunopharmacologyauthors
Jiang Z,Hong Z,Guo W,Xiaoyun G,Gengfa L,Yongning L,Guangxia Xdoi
10.1016/j.intimp.2004.02.004keywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
527-37issue
4eissn
1567-5769issn
1878-1705pii
S1567-5769(04)00054-2journal_volume
4pub_type
杂志文章abstract::There is currently no specific drug for the treatment of sepsis and antibiotic administration is considered the best option, despite numerous issues. Therefore, the development of drugs to control the pathogen-induced inflammatory responses associated with sepsis is essential. To address this, our study examined the t...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.05.051
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:Immune thrombocytopenia (ITP) is an immune-mediated acquired autoimmune hemorrhagic disease. About one-third of patients are unresponsive to first-line therapies. Thalidomide (THD) as an immunomodulatory agent is now used to treat several autoimmune disorders. Therefore, we assessed the safety and efficacy o...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.04.041
更新日期:2019-07-01 00:00:00
abstract::NC/Nga mice are atopic prone mice that can be an animal model for human atopic dermatitis (AD). Dendritic cells (DC) as professional antigen-presenting cells (APC) are the most capable inducers of immune responses. The present study using BALB/c, C57BL/6J, and NC/Nga male mice investigated whether differentiation and ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2008.08.006
更新日期:2008-12-20 00:00:00
abstract::Salmonella typhimurium (ST), as an aggressive bacterium, mainly causes intestinal inflammation and diarrhea. Sodium houttuyfonate (SH) is a derivative of houttuynin in the active oil of Houttuynia cordata, which is stable in nature and has anti-inflammatory activity. In this study, we used BALB/c mice infected with ST...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.107058
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:This study investigated some T helper cytokines' association with different ANA positive sera. METHODS:Sera of 53 ANA-positive subjects (11 were stained of homogeneous pattern, 12 of speckled particle pattern, 9 of nucleolar pattern, 15 of centromere pattern, and 6 of the peripheral nuclear type) were collec...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2014.12.017
更新日期:2015-02-01 00:00:00
abstract::1,25-dihydroxyvitaminD3 [1,25(OH)2D3] modulates both the innate and adaptive immunity in tuberculosis. We explored the effect of 1,25(OH)2D3 on cytolytic molecules like perforin, granulysin, and granzyme-B in T-cells and natural killer cells during M. tuberculosis (Mtb) infection. Peripheral blood mononuclear cells (P...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.07.018
更新日期:2018-09-01 00:00:00
abstract::A novel method of large-scale chromatography has been developed to improve recovery and purity of immunoglobulin G (IgG) from pooled plasma. The current study compares safety, toxicity and efficacy of two intravenous immunoglobulin products: a novel formulation, IGIV caprylate/chromatography (IGIV-C; Gamunex, 10%) and...
journal_title:International immunopharmacology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s1567-5769(03)00134-6
更新日期:2003-09-01 00:00:00
abstract::Dihydrofisetin is a flavanonol derived from some edible wild herbs and traditional Chinese medicines. It has been found to possess many biological activities. However, the anti-inflammatory potential of Dihydrofisetin remains uncharacterized. The aim of the present study was to investigate the anti-inflammatory activi...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2017.11.034
更新日期:2018-01-01 00:00:00
abstract::Influenza viruses cause significant morbidity and mortality and pose a substantial threat to public health. Vaccination represents the principle means of preventing influenza virus infection. Current vaccine approaches are hindered by the need to routinely reformulate vaccine compositions in an effort to account for t...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.07.011
更新日期:2018-10-01 00:00:00
abstract::Acute lung injury (ALI) is one of the most serious complications in critically ill patients which often leads to morbidity and mortality. ALI characterized by severe inflammation of lungs occurs due to uncontrolled inflammatory immune response. However, the immunological mechanism(s) are far from being understood. The...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.12.062
更新日期:2019-03-01 00:00:00
abstract::A focused library of novel mannosylated glycophospholipids was synthesized employing imidate coupling and H-phosphate phosphorylation methods. All novel glycophospholipids were evaluated for their receptor interactions by molecular docking studies. Docking studies revealed dendritic cell-specific intercellular adhesio...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105684
更新日期:2019-09-01 00:00:00
abstract::Tuberculosis (TB) remains as a global health problem. The prevalence of this infection is related to the association with other diseases, such as HIV, neglect treatment and misuse of antibiotics. Hence, the identification of new drugs is required to eradicate TB. Possible alternatives to existing antibiotics include p...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2015.12.027
更新日期:2016-02-01 00:00:00
abstract::We assessed the effects of Zizyphus lotus L. (Desf.) polyphenols (ZLP) on T-cell signaling and proliferation. Our results showed that ZLP exerted no effect on the increases in intracellular free calcium concentrations, [Ca(2+)]i, in human Jurkat T-cells. However, ZLP modulated the thapsigargin-induced increases in [Ca...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2012.11.013
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:The precise role of innate immune responses in the early stage of traumatic spinal cord injury (SCI), especially those mediated by natural killer (NK) cells, is poorly understood. METHODS:The frequency and phenotype of NK cells from traumatic SCI patients and healthy controls were assessed by flow cytometry...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105722
更新日期:2019-09-01 00:00:00
abstract::β-Caryophyllene (BCP) is a bicyclic sesquiterpene compound that has anti-diabetic activity. However, the effect of BCP on diabetic nephropathy (DN) remains unclear. Here, we aimed to evaluate the potential role of BCP in high glucose (HG)-induced glomerular mesangial cells (MCs). MCs were maintained under HG condition...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106556
更新日期:2020-07-01 00:00:00
abstract::Delvestidine (DLTD) is a monomeric compound isolated from Aconitum leucostomum Worosch, a widely used medicine for local treatment of rheumatoid arthritis (RA). Studies have shown that Aconitum leucostomum Worosch. can inhibit maturation of bone marrow-derived dendritic cells (BMDCs). Further, microRNAs (miRNAs) have ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106791
更新日期:2020-11-01 00:00:00
abstract::Glabridin has multiple pharmacological activities including anti-microbial, anti-atherosclerotic, anti-nephritic, anti-inflammatory and cardiovascular protective activities. In this study, we investigated the effect of glabridin on dendritic cells, which play an essential role in innate and adaptive immune responses. ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2010.06.025
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) is attracting considerable attention due to disease acceleration and substantial mortality. Macrophages are known to regulate the fibrotic process in idiopathic pulmonary fibrosis. OBJECTIVE:We investigated if two new macrophage-specific serum bio...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106732
更新日期:2020-09-01 00:00:00
abstract::The therapeutic effects of intravenous immunoglobulin (IVIG) products were recently studied in Alzheimer's disease (AD) patients. Pilot studies produced encouraging results but phase II and III trials gave disappointing results; a further study is in progress. IVIG products contain antibodies to tau protein, the main ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2015.08.022
更新日期:2015-10-01 00:00:00
abstract::Water-soluble acidic polysaccharides from the cell walls of Ulva rigida are mainly composed of disaccharides that contain glucuronic acid and sulphated rhamnose. The structure of disaccharides resembles that of glycosaminoglycans (GAGs) as they both contain glucuronic acid and sulphated sugars. Glycosaminoglycans occu...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2007.02.007
更新日期:2007-07-01 00:00:00
abstract:OBJECTIVES:The purpose of this study is to evaluate the therapeutic efficacy and the role of PD-1/PD-L1 pathway in tongue squamous cell carcinoma (TSCC) patients treated with radical operation combined with chemotherapy and improving cytokine induced killer cells (iCIKs) transfer. METHODS:Thirteen patients who receive...
journal_title:International immunopharmacology
pub_type: 临床试验,杂志文章
doi:10.1016/j.intimp.2018.04.011
更新日期:2018-06-01 00:00:00
abstract::Interleukin (IL)-35 plays an important role in the pathogenesis of rheumatoid arthritis (RA), which is characterized by tumor necrosis factor (TNF)-α activated bone loss beginning early and persisting over time. The aim of this study was to explore the effects and signaling pathway of IL-35 on osteoblasts differentiat...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105810
更新日期:2019-10-01 00:00:00
abstract::Cancers of the lung and pleura remain a major cause of cancer deaths, both in men and women, with strong causal relationships between cigarette smoking and asbestos fibres, and deaths from lung cancer and mesothelioma, respectively. The poor survival rates for small cell lung cancer and mesotheliomas argue powerfully ...
journal_title:International immunopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.intimp.2007.07.013
更新日期:2007-12-20 00:00:00
abstract::This study was designed to determine the level of survivin expression and its clinical significance as a prognostic factor in gastrointestinal stromal sarcoma (GIST). Twenty patients (12 males and 8 females) ranging in age from 25 to 72, with a median age of 53 were evaluated. Failure of TKI treatment was higher in th...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2011.10.005
更新日期:2011-12-01 00:00:00
abstract::Dihydroberberine (DHB), a hydrogenated derivative of berberine (BBR), has been firstly identified in Phellodendri Chinese Cortex (PC) by HPLC-ESI-MS/MS. Nowadays most researches on PC focus on its main components like BBR, however, the role of its naturally-occurring derivatives remains poorly defined heretofore. The ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105802
更新日期:2019-10-01 00:00:00
abstract::Lipopolysaccharide stimulates the intestinal microbiome to activate phosphoinositide 3 kinase (PI3K) signaling via several pathways; however, the direct effect that PI3K has on the intestinal bacterial community remains unclear. Herein, we investigate changes in the colonic microbiome of colitis PI3Kγ-knockout (PI3Kγ-...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105901
更新日期:2020-02-01 00:00:00
abstract::Berberine (BBR) possesses many pharmacological characteristics including anti-inflammation, anti-allergy, anti-angiogenesis and anti-tumor. However, the effects and mechanisms of BBR on IL-33-induced mast cell inflammatory responses are kept unknown. To investigate these, rat peritoneal mast cells (RPMCs) were isolate...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.11.009
更新日期:2019-01-01 00:00:00
abstract::The activities of dectin-1 and mannose receptors on phagocytosis of Candida albicans and the production of TNF-α by macrophages from mice pretreated for 3 days with extract of Artocarpus intergrifolia seeds (jack extract), Artin M or jacalin were studied. Macrophages from these mice were coincubated with C. albicans C...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2011.12.010
更新日期:2012-02-01 00:00:00
abstract::This study was aimed to introduce a novel algorithm for determining linear B- and T-cell epitopes from Crimean-Congo haemorrhagic fever virus (CCHFV) antigens. To this end, 387 approved B- and T-cell epitopes, as well as 331 non-epitope peptides from different serotypes of the virus were collected from IEDB database f...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.106020
更新日期:2020-01-01 00:00:00
abstract::Cartilage degradation is one of the pathological changes of osteoarthritis (OA), and accumulating evidence suggests an excess of matrix metalloproteinases (MMPs) plays a role in this cartilage breakdown. Here, we investigated the effects of chlorogenic acid (CGA) on the mRNA and protein expression of MMPs in interleuk...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2010.09.021
更新日期:2011-01-01 00:00:00