Abstract:
:The safety and efficacy of imiquimod 5% cream is being evaluated for the treatment of dysplastic lesions of the epidermis (actinic keratoses, AK). The objective of this clinical study was to describe the pharmacokinetics and safety of topical imiquimod during multiple dosing of AK subjects. A total of 58 adult subjects with 5 to 20 AK lesions at the treatment site applied imiquimod cream three times per week for up to 16 weeks as follows: 12 males and 11 females applied 12.5 mg imiquimod to the face; 11 males applied 25 mg to the entire balding area of the scalp; and 12 males and 12 females applied 75 mg to both hands and forearms. Pharmacokinetics and safety were assessed after the first and last doses, as well as biweekly. Imiquimod and its metabolites were measured in the serum and urine using sensitive liquid chromatography/mass spectrometry methods. Less than 0.6% of the applied doses was recovered in the urine of all subjects. Serum imiquimod levels were low, reflecting minimal dermal absorption, and increased with dose, although not proportionally. Peak levels at the end of dosing were 0.1, 0.2, and 1.6 ng/ml for the face, scalp, and hands/arms groups, respectively. A two- to fourfold accumulation was seen at the end of dosing. Local application site reactions were the most common adverse event, reported by approximately 50% of the subjects in each treatment group. The small number of systemic adverse events, including 'flu-like symptoms, were mostly mild and did not show a dose response. Thus, minimal systemic absorption and good safety margins for topical imiquimod were seen in AK subjects with doses as high as 75 mg three times per week for 16 weeks.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Harrison LI,Skinner SL,Marbury TC,Owens ML,Kurup S,McKane S,Greene RJdoi
10.1007/s00403-004-0465-4keywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
6-11issue
1eissn
0340-3696issn
1432-069Xjournal_volume
296pub_type
临床试验,杂志文章,多中心研究abstract::The composition of the aerobic and anaerobic bacterial skin flora was investigated quantitatively and qualitatively in 25 patients with seborrheic eczema (SE) and in 35 healthy persons. In SE patients, the geometric mean count for propionibacteria in the pilosebaceous ducts of the forehead was reduced to a statistical...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404292
更新日期:1980-01-01 00:00:00
abstract::Metalloproteinase-mediated proteolysis plays an important role during all stages of wound repair. In acute murine wounds, murine collagenase, matrix metalloproteinase (MMP)-9, stromelysin-1, stromelysin-2 and tissue inhibitor of metalloproteinase (TIMP)-1 are strongly induced within 24 h after wounding. At the time of...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/pl00007450
更新日期:1998-07-01 00:00:00
abstract::This study was designed in order to prove on a large-scale basis the efficacy of oral photochemotherapy (PUVA) in the prevention of polymorphous light eruption (PLE), to work out indication criteria for PUVA treatment of this disease, and to establish a simple method based on anamnestic data to differentiate UVA from ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417337
更新日期:1983-01-01 00:00:00
abstract::Koebner response was studied in 35 psoriatic patients. Two punch biopsies per patient were taken from non-lesional psoriatic skin before, and 6 h, 2 days, 7 days, 14 days and 21 days after, tape stripping. Alterations in the numbers of CD1+ Langerhans cells, CD4+ and CD8+ T cells and CD11c+ macrophages were mapped mor...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372065
更新日期:1992-01-01 00:00:00
abstract::Acrolein and 4-hydroxy-2-nonenal (HNE) are both byproducts of a lipid peroxidation reaction. Actinic elastosis in photodamaged skin of aged individuals is characterized by the accumulation of fragmented elastic fibers in the sun-exposed areas. To study whether a lipid peroxidation reaction is involved in the accumulat...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030100239
更新日期:2001-07-01 00:00:00
abstract::Flushing in rosacea has been investigated by means of (a) pharmacological inhibition of some possible chemical mediators and (b) titration of bradykinin as a possible effector directly in the blood. Clonidine-inhibited flushing was seen in all patients (mean 45%), other drugs had poorer results. Bradykinin increased i...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509061
更新日期:1982-01-01 00:00:00
abstract::In this study, a new procedure for the cultivation of dermal vascular endothelial cells from guinea pig ear lobe was established, and specific binding of [3H]mepyramine to the cultured cell membranes was demonstrated. The vascular endothelial cells were successfully isolated from the dermis of guinea pig ear lobes by ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00493469
更新日期:1990-01-01 00:00:00
abstract::In this study we investigated the influence of keratinocytes on the phenotype of fibroblasts in an in vitro human skin equivalent. Keratinocytes were seeded at the surface of fibroblast-populated mechanically restrained type I collagen gels (lattices). Lattices without keratinocytes were handled in parallel as control...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371739
更新日期:1995-01-01 00:00:00
abstract::Six patients with delayed pressure urticaria (DPU) applied clobetasol propionate (0.05%) ointment or its base to predetermined test sites on the right and left thigh as part of a randomized, double-blind study. A pressure challenge was administered to each test site at the initial visit and repeated after 3 days and 6...
journal_title:Archives of dermatological research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF01105080
更新日期:1995-01-01 00:00:00
abstract::Atopic dermatitis (AD) is a frequent and burdensome disease. The objectives of this study were (1) to assess the willingness to pay (WTP) and quality of life (Qol) in AD patients and (2) to compare the results with data on other chronic skin diseases. To collect data, a non-interventional, cross-sectional nationwide p...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-013-1402-1
更新日期:2014-04-01 00:00:00
abstract::The mechanical properties of the in vivo dermis were measured by means of a torque applied to the skin. The resulting deformation of 2-6 degrees, including the immediate and delayed visco-elastic components, as well as the relaxation were measured, and the raw values corrected for a constant skin thickness. The experi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00406415
更新日期:1980-01-01 00:00:00
abstract::The phospholipids of the skin are difficult to quantify because they represent only a small fraction of the skin tissue. In this study, 31P nuclear magnetic resonance, which permits precise profiling of these phospholipids, was used to compare the phospholipids of upper eyelid epidermal and dermal lipid extracts (n = ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050308
更新日期:1998-06-01 00:00:00
abstract::Typical confluent lesions of lichen planus were transplanted onto nude mice and cultured in organ culture. The characteristic histologic appearance of lichen planus disappeared after grafting and became similar to normal skin within 6 weeks on nude mice; the dense lymphocyte infiltrate in dermis disappeared, the basal...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412684
更新日期:1988-01-01 00:00:00
abstract::No previous studies have convincingly linked sweating disturbance with the subsequent development of lichen planus (LP). Therefore, we investigated whether sweating disturbance could be specifically detected in LP lesions and how it could trigger lesion development. We utilized the impression mold technique (IMT), whi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-018-1882-0
更新日期:2019-01-01 00:00:00
abstract::In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore e...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-0979-x
更新日期:2010-04-01 00:00:00
abstract::The C1-inactivator plays an important role not only in the initial phases of the complement system, but also in those of the coagulation, fibrinolysis and kinin systems. The present study was concerned with the reciprocal influence of decreased C1-inactivator levels in patients with hereditary angioneurotic edema (HAN...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00413358
更新日期:1984-01-01 00:00:00
abstract::Mice homozygous for three different recessive mutations known to cause pleiotropic defects in the immune system and in the skin were used to evaluate the relationship between the classical immune system and dendritic epidermal cell populations. Numbers of Langerhans cells (LCs) and Thy-1+ dendritic cells (Thy-1+DEC) w...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372621
更新日期:1990-01-01 00:00:00
abstract::Epidermolytic hyperkeratosis (EH) is a genetic disorder of keratins associated with epidermal differentiation. Affected individuals carry gene mutations for conserved sequences of keratins K1 or K10. The structural alterations of tonofilaments in EH seem to be a direct consequence of the keratin gene mutations. EH epi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF01105793
更新日期:1995-01-01 00:00:00
abstract::To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers an...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00375800
更新日期:1992-01-01 00:00:00
abstract::Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1606-7
更新日期:2016-01-01 00:00:00
abstract::Heparin-binding EGF-like growth factor (HB-EGF) and amphiregulin (AREG) are the members of EGF family that bind to common EGF receptor (EGFR) in the epidermis. However, the role of these two growth factors in epidermal hyperplasia of psoriasis has not been established. On the other hand, CD4+ T cells are responsible f...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-007-0809-y
更新日期:2008-01-01 00:00:00
abstract::As the main nitrogen source in Malassezia furfur, tryptophan induces the formation of fluorochromes and pigments, which make the yeast less sensitive to UV light. To detect a chemical UV filter, M. furfur (CBS 1878) was incubated at 30 degrees C for 14 days on a pigment-inducing medium and agar extracts were purified ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-002-0294-2
更新日期:2002-05-01 00:00:00
abstract::Investigations on the nutrient requirements of Malassezia yeasts led to the preparation of a new minimal medium consisting of an amino nitrogen and a lipid source, which only allowed the growth of the species M. furfur. Carbohydrates, electrolytes, vitamins and trace elements were not required. Using the basal medium,...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050304
更新日期:1998-05-01 00:00:00
abstract::To elucidate the interaction between melanoma and its matrix, we cultured B16 murine melanoma cells on and in type I collagen gel and evaluated specified functions of melanoma cells; tyrosinase activity and melanin-synthesizing capacity. Proliferation of cells cultured in these environments was markedly suppressed com...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371647
更新日期:1990-01-01 00:00:00
abstract::Patients with certain inflammatory skin diseases have multiple risk factors for venous thromboembolism (VTE). The objective of the study was to determine whether atopic dermatitis (AD), psoriasis, pemphigus, pemphigoid and/or hidradenitis is associated with VTE in US adults. Data were analyzed from the 2002-2012 Natio...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-020-02099-6
更新日期:2020-07-08 00:00:00
abstract::Melanoma in situ (MIS) is a form of radial growth phase melanoma in which the proliferation of malignant cells is confined to the epidermis. Histologic features are invaluable in recognition of MIS. Regression occurs when the host's immune system attacks the primary melanocytic tumor cells via tumor infiltrate lymphoc...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-020-02106-w
更新日期:2020-07-06 00:00:00
abstract::Psoriasis was initially considered to represent a disease of abnormal epidermal keratinocyte proliferation. Proliferation of keratinocytes is restricted by apoptotic cell death to maintain a constant thickness of epidermis. Nucleosomes are mainly released by apoptotic cells. Tumor necrosis factor-α (TNF-α) is an impor...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-013-1347-4
更新日期:2013-08-01 00:00:00
abstract::Skin topography and microvasculature undergo characteristic changes with age. Although several non-invasive bioengineering methods are currently available to measure them quantitatively, few publications have referred to their relationship with age in different anatomical sites. This study was carried out to observe t...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0628-y
更新日期:2006-03-01 00:00:00
abstract::We investigated the effects of glucocorticoids on IL-3-dependent proliferation and c-kit expression of cells of the mouse mast cell line, MC/9. Glucocorticoids (dexamethasone, prednisolone, and hydrocortisone) inhibited IL-3-dependent MC/9 cell proliferation, whereas sex steroids (progesterone, beta-estradiol, and tes...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050398
更新日期:1999-04-01 00:00:00
abstract::Serotonin is a monoamine acting as a neuromediator in the central and peripheral nervous system. Recently, serotonin has also been shown to influence T- and B-cell function. The serotonin transporter is central in the regulation of the serotonergic system and widely expressed on cells of the immune system. A functiona...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-008-0909-3
更新日期:2009-07-01 00:00:00